SRB2/YHR041C Protein Information

Standard Name	Srb2p 1
Systematic Name	Yhr041cp
Alias	Hrs2p , Med20p 2
ORF Classification	Verified
Description	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance (1, 3, 4, 5, 6, 7)
Name Description	Suppressor of RNA polymerase B 1

Experimental Data
Molecules/cell	1720 8

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	210
Molecular Weight (Da)	22,894
Isoelectric Point (pI)	5.15

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Srb2p (InterPro)
Physical Interactions	There are 123 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000005315B \| P34162 MIPS: YHR041C NCBI: 116667901 \| 116667903 \| 116667905 \| 116667907 \| 116667909 \| 116667911 \| 116667913 \| 116667915 \| 249687 \| 345100889 \| 345100896 \| 345100903 \| 410563054 \| 410563061 \| 459358311 \| 464805 \| 488185 \| 6321831 \| NP_011907.1 GenBank/EMBL/DDBJ: DAA06733.1 \| S36754 \| U00062

Amino Acid Sequence (or in FASTA format)
1 MGKSAVIFVE RATPATLTEL KDALSNSILS VRDPWSIDFR TYRCSIKNLP 51 ADVSKLMYSI TFHHHGRQTV LIKDNSAMVT TAAAADIPPA LVFNGSSTGV 101 PESIDTILSS KLSNIWMQRQ LIKGDAGETL ILDGLTVRLV NLFSSTGFKG 151 LLIELQADEA GEFETKIAGI EGHLAEIRAK EYKTSSDSLG PDTSNEICDL 201 AYQYVRALEL *

Amino Acid Sequence (or in FASTA format)

       1  MGKSAVIFVE RATPATLTEL KDALSNSILS VRDPWSIDFR TYRCSIKNLP
      51  ADVSKLMYSI TFHHHGRQTV LIKDNSAMVT TAAAADIPPA LVFNGSSTGV
     101  PESIDTILSS KLSNIWMQRQ LIKGDAGETL ILDGLTVRLV NLFSSTGFKG
     151  LLIELQADEA GEFETKIAGI EGHLAEIRAK EYKTSSDSLG PDTSNEICDL
     201  AYQYVRALEL *

external links for Srb2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Srb2p

1)	Nonet ML and Young RA (1989) Intragenic and extragenic suppressors of mutations in the heptapeptide repeat domain of Saccharomyces cerevisiae RNA polymerase II. Genetics 123(4):715-24
2)	Bourbon HM, et al. (2004) A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. Mol Cell 14(5):553-7
3)	Thompson CM, et al. (1993) A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast. Cell 73(7):1361-75
4)	Myers LC, et al. (1998) The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain. Genes Dev 12(1):45-54
5)	Kim YJ, et al. (1994) A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II. Cell 77(4):599-608
6)	Kornberg RD (2005) Mediator and the mechanism of transcriptional activation. Trends Biochem Sci 30(5):235-9
7)	Askree SH, et al. (2004) A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length. Proc Natl Acad Sci U S A 101(23):8658-63
8)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41