PUT2/YHR037W Protein Information

Standard Name	Put2p 1
Systematic Name	Yhr037wp
ORF Classification	Verified
Description	Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism (2, 3)
Name Description	Proline UTilization 1

Experimental Data
Molecules/cell	17200 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	575
Molecular Weight (Da)	64,435
Isoelectric Point (pI)	6.99

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Put2p (InterPro)
Physical Interactions	There are 5 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000132B7E \| P07275 MIPS: YHR037W NCBI: 172303 \| 488172 \| 6321826 \| 730430 \| NP_011902.1 GenBank/EMBL/DDBJ: DAA06728.1 \| M10029 \| M22785 \| U00062
External Classifications	EC: 1.5.1.12 [1-pyrroline-5-carboxylate dehydrogenase]

Amino Acid Sequence (or in FASTA format)
1 MLSARCLKSI YFKRSFSQLG HIKPPKHIRN EPVKPFRNID LKDWDLLRAS 51 LMKFKSSSLE VPLVINGERI YDNNERALFP QTNPANHQQV LANVTQATEK 101 DVMNAVKAAK DAKKDWYNLP FYDRSAIFLK AADLISTKYR YDMLAATMLG 151 QGKNVYQAEI DCITELSDFF RYYVKYASDL YAQQPVESAD GTWNKAEYRP 201 LEGFVYAVSP FNFTAIAANL IGAPALMGNT VVWKPSQTAA LSNYLLMTVL 251 EEAGLPKGVI NFIPGDPVQV TDQVLADKDF GALHFTGSTN VFKSLYGKIQ 301 SGVVEGKYRD YPRIIGETGG KNFHLVHPSA NISHAVLSTI RGTFEFQGQK 351 CSAASRLYLP ESKSEEFLSD MFGILQSQNV VPMNTSASPI SGGNLRGFMG 401 PVIHEQSFDK LVKVIEDAKK DPELEILYGG QYDKSQGWFV GPTVIKAKRP 451 DHPYMSTEFF GPILTVYEYP DTEFNEICDI IDNTSQYALT GAIFAKDRKA 501 IEYADEKLKF SAGNFYINDK CTGAVVSQQW FGGARMSGTD DKAGGPNILS 551 RFVSIRNTKE NFYELTDFKY PSNYE*

Amino Acid Sequence (or in FASTA format)

       1  MLSARCLKSI YFKRSFSQLG HIKPPKHIRN EPVKPFRNID LKDWDLLRAS
      51  LMKFKSSSLE VPLVINGERI YDNNERALFP QTNPANHQQV LANVTQATEK
     101  DVMNAVKAAK DAKKDWYNLP FYDRSAIFLK AADLISTKYR YDMLAATMLG
     151  QGKNVYQAEI DCITELSDFF RYYVKYASDL YAQQPVESAD GTWNKAEYRP
     201  LEGFVYAVSP FNFTAIAANL IGAPALMGNT VVWKPSQTAA LSNYLLMTVL
     251  EEAGLPKGVI NFIPGDPVQV TDQVLADKDF GALHFTGSTN VFKSLYGKIQ
     301  SGVVEGKYRD YPRIIGETGG KNFHLVHPSA NISHAVLSTI RGTFEFQGQK
     351  CSAASRLYLP ESKSEEFLSD MFGILQSQNV VPMNTSASPI SGGNLRGFMG
     401  PVIHEQSFDK LVKVIEDAKK DPELEILYGG QYDKSQGWFV GPTVIKAKRP
     451  DHPYMSTEFF GPILTVYEYP DTEFNEICDI IDNTSQYALT GAIFAKDRKA
     501  IEYADEKLKF SAGNFYINDK CTGAVVSQQW FGGARMSGTD DKAGGPNILS
     551  RFVSIRNTKE NFYELTDFKY PSNYE*

external links for Put2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	GeneMANIA
YOGY

References cited on this page View Complete Literature Guide for Put2p

1)	Brandriss MC and Magasanik B (1979) Genetics and physiology of proline utilization in Saccharomyces cerevisiae: enzyme induction by proline. J Bacteriol 140(2):498-503
2)	Brandriss MC (1983) Proline utilization in Saccharomyces cerevisiae: analysis of the cloned PUT2 gene. Mol Cell Biol 3(10):1846-56
3)	Hu CA, et al. (1996) Cloning, characterization, and expression of cDNAs encoding human delta 1-pyrroline-5-carboxylate dehydrogenase. J Biol Chem 271(16):9795-800
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41