MYO1/YHR023W Protein Information

Standard Name	Myo1p
Systematic Name	Yhr023wp
ORF Classification	Verified
Description	Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively (1, 2, 3, 4)
Name Description	MYOsin

Experimental Data
Molecules/cell	2140 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,928
Molecular Weight (Da)	223,634
Isoelectric Point (pI)	6.35

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Myo1p (InterPro)
Physical Interactions	There are 107 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052F6E \| P08964 MIPS: YHR023W NCBI: 4016 \| 4018 \| 500691 \| 6321812 \| 730092 \| NP_011888.1 \| NM_001179153.1 GenBank/EMBL/DDBJ: DAA06714.1 \| U10399 \| X06187 \| X53947

Amino Acid Sequence (or in FASTA format)
1 MTGGQSCSSN MIVWIPDEKE VFVKGELMST DINKNKFTGQ EEQIGIVHPL 51 DSTEVSNLVQ VRISDVFPVN PSTFDKVENM SELTHLNEPS VLYNLEKRYD 101 CDLIYTYSGL FLVAINPYHN LNLYSEDHIN LYHNKHNRLS KSRLDENSHE 151 KLPPHIFAIA EEAYENLLSE GKDQSILVTG ESGAGKTENT KKILQYLASI 201 TSGSPSNIAP VSGSSIVESF EMKILQSNPI LESFGNAQTV RNNNSSRFGK 251 FIKIEFNEHG MINGAHIEWY LLEKSRIVHQ NSKERNYHIF YQLLSGLDDS 301 ELKNLRLKSR NVKDYKILSN SNQDIIPGIN DVENFKELLS ALNIIGFSKD 351 QIRWIFQVVA IILLIGNIEF VSDRAEQASF KNDVSAICSN LGVDEKDFQT 401 AILRPRSKAG KEWVSQSKNS QQAKFILNAL SRNLYERLFG YIVDMINKNL 451 DHGSATLNYI GLLDIAGFEI FENNSFEQLC INYTNEKLQQ FFNNHMFVLE 501 QSEYLKENIQ WDYIDYGKDL QLTIDLIESK GPPTGVLPLL DEEAVLPKST 551 DESFYSKLIS TWDQNSSKFK RSRLKNGFIL KHYAGDVEYT VEGWLSKNKD 601 PLNDNLLSLL SSSQNDIISK LFQPEGEKSS SAGVEANISN QEVKKSARTS 651 TFKTTSSRHR EQQITLLNQL ASTHPHFVRC IIPNNVKKVK TFNRRLILDQ 701 LRCNGVLEGI RLAREGYPNR IAFQEFFQRY RILYPENSTT TTFSSKLKAS 751 TKQNCEFLLT SLQLDTKVYK IGNTKLFFKA GVLADLEKQK DVKLNNIMIK 801 LTATIRGYTV RKEITYHLQK LKKTRVIGNT FRLYNRLVKE DPWFNLFIRI 851 KPLLTSSNDM TRTKKFNEQI NKLKNDLQEM ESKKKFLEEK NQKTVNELEN 901 TQDLLNQEKE NLRKNESLLN RVKTSSETLQ KQFDDLVSEK DEISREKLEV 951 AQNLEEAHQK IQGLQETIRE REATLEKLHS KNNELIKQIS DLNCDISKEQ 1001 SSQSLIKESK LKLENEIKRL KDVINSKEEE IKSFNDKLSS SEEDLDIKLV 1051 TLEKNCNIAM SRLQSLVTEN SDLRSKNENF KKEKAALNNQ LKNKESELLK 1101 MKEKIDNHKK ELATFSKQRD DAVSEHGKIT AELKETRIQL TEYKSNYQKI 1151 KEEYSNFQRE TKEQEQKKRN SLVESLNDSK IKELEARLSQ EISLNQYLNK 1201 RISGNSVETN ISSTRRSTSY SDDPLDKEDI IKKYYDLQLA FTEITRNLEN 1251 EIEEKKNLIS RLRFTETRLA SSSFEDQKIK AQMKKLKKLI QDMDPSIPLD 1301 SILNEPLDNC PDKESDINKL MLEVDYLKRQ LDIETRAHYD AENAISALHS 1351 KFRKIQGESS LSSSDIYKLK FEASEERVKS LEDKLKTMPL RDRTNLPVGD 1401 IIKNRDSISK YEEEIRYYKL ENYKLQEILN ESNGKLSQLT LDLRQSKSKE 1451 ALLSEQLDRL QKDLESTERQ KELLSSTIKQ QKQQFENCMD DLQGNELRLR 1501 EHIHALKQAE EDVKNMASII EKLKTQNKQK EKLIWEREME RNDSDMQLQE 1551 TLLELKRVQD VKKILSDDLA HLKERLSAVE DRSQYTDEIN RLKEELNCSL 1601 KAETNLKKEF ATLKYKLETS TNDSEAKISD LLKQLDHYTK VVEMLNNEKD 1651 AISLAEKELY QKYEALNTEC ESLKGKIVSL TKIKQELESD LNQKTDALQI 1701 SNAALSSSTQ KNKEITEKIK YLEETLQLQM EQNSRNGELV KTLQASCNGY 1751 KDKFDDEKQK NIDLYEENQT LQKLNTDLQL QLKNLHERLS DTTEKNAWLS 1801 KIHELENMVS LETDLKYEEM KKNKSLERAV EELQTKNSQQ TDVIELANKN 1851 RSEFEEATLK YEAQISDLEK YISQQELEMK KSIRDNSSYR DKVQEMAQEI 1901 EFWKSRYEST MIGSKNIDSN NAQSKIFS*

Amino Acid Sequence (or in FASTA format)

       1  MTGGQSCSSN MIVWIPDEKE VFVKGELMST DINKNKFTGQ EEQIGIVHPL
      51  DSTEVSNLVQ VRISDVFPVN PSTFDKVENM SELTHLNEPS VLYNLEKRYD
     101  CDLIYTYSGL FLVAINPYHN LNLYSEDHIN LYHNKHNRLS KSRLDENSHE
     151  KLPPHIFAIA EEAYENLLSE GKDQSILVTG ESGAGKTENT KKILQYLASI
     201  TSGSPSNIAP VSGSSIVESF EMKILQSNPI LESFGNAQTV RNNNSSRFGK
     251  FIKIEFNEHG MINGAHIEWY LLEKSRIVHQ NSKERNYHIF YQLLSGLDDS
     301  ELKNLRLKSR NVKDYKILSN SNQDIIPGIN DVENFKELLS ALNIIGFSKD
     351  QIRWIFQVVA IILLIGNIEF VSDRAEQASF KNDVSAICSN LGVDEKDFQT
     401  AILRPRSKAG KEWVSQSKNS QQAKFILNAL SRNLYERLFG YIVDMINKNL
     451  DHGSATLNYI GLLDIAGFEI FENNSFEQLC INYTNEKLQQ FFNNHMFVLE
     501  QSEYLKENIQ WDYIDYGKDL QLTIDLIESK GPPTGVLPLL DEEAVLPKST
     551  DESFYSKLIS TWDQNSSKFK RSRLKNGFIL KHYAGDVEYT VEGWLSKNKD
     601  PLNDNLLSLL SSSQNDIISK LFQPEGEKSS SAGVEANISN QEVKKSARTS
     651  TFKTTSSRHR EQQITLLNQL ASTHPHFVRC IIPNNVKKVK TFNRRLILDQ
     701  LRCNGVLEGI RLAREGYPNR IAFQEFFQRY RILYPENSTT TTFSSKLKAS
     751  TKQNCEFLLT SLQLDTKVYK IGNTKLFFKA GVLADLEKQK DVKLNNIMIK
     801  LTATIRGYTV RKEITYHLQK LKKTRVIGNT FRLYNRLVKE DPWFNLFIRI
     851  KPLLTSSNDM TRTKKFNEQI NKLKNDLQEM ESKKKFLEEK NQKTVNELEN
     901  TQDLLNQEKE NLRKNESLLN RVKTSSETLQ KQFDDLVSEK DEISREKLEV
     951  AQNLEEAHQK IQGLQETIRE REATLEKLHS KNNELIKQIS DLNCDISKEQ
    1001  SSQSLIKESK LKLENEIKRL KDVINSKEEE IKSFNDKLSS SEEDLDIKLV
    1051  TLEKNCNIAM SRLQSLVTEN SDLRSKNENF KKEKAALNNQ LKNKESELLK
    1101  MKEKIDNHKK ELATFSKQRD DAVSEHGKIT AELKETRIQL TEYKSNYQKI
    1151  KEEYSNFQRE TKEQEQKKRN SLVESLNDSK IKELEARLSQ EISLNQYLNK
    1201  RISGNSVETN ISSTRRSTSY SDDPLDKEDI IKKYYDLQLA FTEITRNLEN
    1251  EIEEKKNLIS RLRFTETRLA SSSFEDQKIK AQMKKLKKLI QDMDPSIPLD
    1301  SILNEPLDNC PDKESDINKL MLEVDYLKRQ LDIETRAHYD AENAISALHS
    1351  KFRKIQGESS LSSSDIYKLK FEASEERVKS LEDKLKTMPL RDRTNLPVGD
    1401  IIKNRDSISK YEEEIRYYKL ENYKLQEILN ESNGKLSQLT LDLRQSKSKE
    1451  ALLSEQLDRL QKDLESTERQ KELLSSTIKQ QKQQFENCMD DLQGNELRLR
    1501  EHIHALKQAE EDVKNMASII EKLKTQNKQK EKLIWEREME RNDSDMQLQE
    1551  TLLELKRVQD VKKILSDDLA HLKERLSAVE DRSQYTDEIN RLKEELNCSL
    1601  KAETNLKKEF ATLKYKLETS TNDSEAKISD LLKQLDHYTK VVEMLNNEKD
    1651  AISLAEKELY QKYEALNTEC ESLKGKIVSL TKIKQELESD LNQKTDALQI
    1701  SNAALSSSTQ KNKEITEKIK YLEETLQLQM EQNSRNGELV KTLQASCNGY
    1751  KDKFDDEKQK NIDLYEENQT LQKLNTDLQL QLKNLHERLS DTTEKNAWLS
    1801  KIHELENMVS LETDLKYEEM KKNKSLERAV EELQTKNSQQ TDVIELANKN
    1851  RSEFEEATLK YEAQISDLEK YISQQELEMK KSIRDNSSYR DKVQEMAQEI
    1901  EFWKSRYEST MIGSKNIDSN NAQSKIFS*

external links for Myo1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Myo1p

1)	Bi E, et al. (1998) Involvement of an actomyosin contractile ring in Saccharomyces cerevisiae cytokinesis. J Cell Biol 142(5):1301-12
2)	Brown SS (1997) Myosins in yeast. Curr Opin Cell Biol 9(1):44-8
3)	Tolliday N, et al. (2003) Direct evidence for a critical role of myosin II in budding yeast cytokinesis and the evolvability of new cytokinetic mechanisms in the absence of myosin II. Mol Biol Cell 14(2):798-809
4)	Luo J, et al. (2004) Identification and functional analysis of the essential and regulatory light chains of the only type II myosin Myo1p in Saccharomyces cerevisiae. J Cell Biol 165(6):843-55
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41