YHR020W Protein Information Help

Systematic Name Yhr020wp
ORF Classification Verified
Description Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments (1, 2, 3, 4)
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 688
Molecular Weight (Da) 77,386
Isoelectric Point (pI) 6.36

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Yhr020wp (InterPro)
Physical Interactions There are 39 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052E8D | P38708
MIPS: YHR020W
NCBI: 500692 | 6321808 | 731640 | NP_011884.1
GenBank/EMBL/DDBJ: DAA06709.1 | U10399
External Classifications EC: 6.1.1.15 [Proline--tRNA ligase]
Amino Acid Sequence (or in FASTA format)
 
       1  MPVSEAFAKL CVNEKPPAES AVAVKSLVFK PKTPKSATPV PIVVVALQST
      51  TTPSALIANA TSSKDPRLAR DDLVKQAFQS ESARAFILGD LANATSNFHL
     101  LIDHELGTVD GDTILQLNDS TYMKKSDMMK FLNNFEDSQK VVDFSQEVSK
     151  ETATEGKKQQ KKQQPSKAGT AAAAAAAALE DAKLIGITVD KALDFPGWYQ
     201  QILTKGEMLD YYDVSGCYIL RPPSYAIWEN IQKWFDDKIK AIGVQNAYFP
     251  MFVSSRVLEK EKDHVEGFAP EVAWVTRAGS SELEEPIAIR PTSETVMYPY
     301  YAKWVQSYRD LPLKLNQWNS VVRWEFKHPQ PFLRTREFLW QEGHTAHLTA
     351  KDAEEEVLQI LDFYAGVYEE LLAVPVVKGR KTEKEKFAGG DFTTTCEGYI
     401  PQTGRGIQGA TSHHLGQNFS KMFNLSVENP LGSDHPKIFA YQNSWGLSTR
     451  VIGVMVMIHS DNKGLVIPPR VSQFQSVVIP VGITKKTSEE QRKHIHETAR
     501  SVESRLKKVG IRAFGDYNDN YTPGWKFSQY ELKGIPIRIE LGPKDIEKNQ
     551  VVVVRRNDSK KYVVSFDELE ARIPEILEEM QGDLFKKAKE LFDTHRVIVN
     601  EWSGFVPALN KKNVILAPWC GVMECEEDIK ESSAKKDDGE EFEEDDKAPS
     651  MGAKSLCIPF DQPVLNEGQK CIKCERIAVN YCMFGRSY*

external links for Yhr020wp
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA


YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Yhr020wp
1) Tatusov RL, et al.  (2000) The COG database: a tool for genome-scale analysis of protein functions and evolution. Nucleic Acids Res 28(1):33-6
2) Fleischer TC, et al.  (2006) Systematic identification and functional screens of uncharacterized proteins associated with eukaryotic ribosomal complexes. Genes Dev 20(10):1294-307
3) Sternjohn J, et al.  (2007) Restoring species-specific posttransfer editing activity to a synthetase with a defunct editing domain. Proc Natl Acad Sci U S A 104(7):2127-32
4) Hati S, et al.  (2006) Pre-transfer editing by class II prolyl-tRNA synthetase: role of aminoacylation active site in "selective release" of noncognate amino acids. J Biol Chem 281(38):27862-72