GPA1/YHR005C Protein Information Help

Standard Name Gpa1p 1
Systematic Name Yhr005cp
Alias Cdc70p 2 , Dac1p 3 , Scg1p 4
ORF Classification Verified
Description Subunit of the G protein involved in pheromone response; GTP-binding alpha subunit of the heterotrimeric G protein; negatively regulates the mating pathway by sequestering G(beta)gamma and by triggering an adaptive response; activates Vps34p at the endosome; protein abundance increases in response to DNA replication stress (5, 6, 7, 8)
Name Description G Protein Alpha subunit 1
Experimental Data
Molecules/cell 9920 9
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 472
Molecular Weight (Da) 54,075
Isoelectric Point (pI) 7.58

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Gpa1p (InterPro)
Physical Interactions There are 68 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000016833F | P08539
MIPS: YHR005C
NCBI: 120991 | 171613 | 173560 | 500823 | 51013377 | 6321792 | NP_011868.1
GenBank/EMBL/DDBJ: DAA06692.1 | AY692963 | M15867 | M17414 | U10555
Amino Acid Sequence (or in FASTA format)
 
       1  MGCTVSTQTI GDESDPFLQN KRANDVIEQS LQLEKQRDKN EIKLLLLGAG
      51  ESGKSTVLKQ LKLLHQGGFS HQERLQYAQV IWADAIQSMK ILIIQARKLG
     101  IQLDCDDPIN NKDLFACKRI LLKAKALDYI NASVAGGSDF LNDYVLKYSE
     151  RYETRRRVQS TGRAKAAFDE DGNISNVKSD TDRDAETVTQ NEDADRNNSS
     201  RINLQDICKD LNQEGDDQMF VRKTSREIQG QNRRNLIHED IAKAIKQLWN
     251  NDKGIKQCFA RSNEFQLEGS AAYYFDNIEK FASPNYVCTD EDILKGRIKT
     301  TGITETEFNI GSSKFKVLDA GGQRSERKKW IHCFEGITAV LFVLAMSEYD
     351  QMLFEDERVN RMHESIMLFD TLLNSKWFKD TPFILFLNKI DLFEEKVKSM
     401  PIRKYFPDYQ GRVGDAEAGL KYFEKIFLSL NKTNKPIYVK RTCATDTQTM
     451  KFVLSAVTDL IIQQNLKKIG II*

external links for Gpa1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Gpa1p
1) Nakafuku M, et al.  (1987) Occurrence in Saccharomyces cerevisiae of a gene homologous to the cDNA coding for the alpha subunit of mammalian G proteins. Proc Natl Acad Sci U S A 84(8):2140-4
2) Jahng KY, et al.  (1988) Mutations in a gene encoding the alpha subunit of a Saccharomyces cerevisiae G protein indicate a role in mating pheromone signaling. Mol Cell Biol 8(6):2484-93
3) Fujimura HA  (1989) The yeast G-protein homolog is involved in the mating pheromone signal transduction system. Mol Cell Biol 9(1):152-8
4) Dietzel C and Kurjan J  (1987) The yeast SCG1 gene: a G alpha-like protein implicated in the a- and alpha-factor response pathway. Cell 50(7):1001-10
5) Dohlman HG and Thorner JW  (2001) Regulation of G protein-initiated signal transduction in yeast: paradigms and principles. Annu Rev Biochem 70():703-54
6) Guo M, et al.  (2003) The yeast G protein alpha subunit Gpa1 transmits a signal through an RNA binding effector protein Scp160. Mol Cell 12(2):517-24
7) Slessareva JE, et al.  (2006) Activation of the phosphatidylinositol 3-kinase Vps34 by a G protein alpha subunit at the endosome. Cell 126(1):191-203
8) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
9) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41