NEM1/YHR004C Protein Information

Standard Name	Nem1p 1
Systematic Name	Yhr004cp
ORF Classification	Verified
Description	Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard (1, 2, 3)
Name Description	Nuclear Envelope Morphology 1

Experimental Data
Molecules/cell	377 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	446
Molecular Weight (Da)	50,641
Isoelectric Point (pI)	9.62

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Nem1p (InterPro)
Transmembrane Domains	There are 1 total predicted transmembrane domains (TMHMM)
Physical Interactions	There are 4 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052E73 \| P38757 MIPS: YHR004C NCBI: 500822 \| 51013351 \| 6321791 \| 731630 \| NP_011867.1 GenBank/EMBL/DDBJ: DAA06691.1 \| AY692950 \| U10555
External Classifications	EC: 3.1.3.16 [Phosphoprotein phosphatase]

Amino Acid Sequence (or in FASTA format)
1 MNALKYFSNH LITTKKQKKI NVEVTKNQDL LGPSKEVSNK YTSHSENDCV 51 SEVDQQYDHS SSHLKESDQN QERKNSVPKK PKALRSILIE KIASILWALL 101 LFLPYYLIIK PLMSLWFVFT FPLSVIERRV KHTDKRNRGS NASENELPVS 151 SSNINDSSEK TNPKNCNLNT IPEAVEDDLN ASDEIILQRD NVKGSLLRAQ 201 SVKSRPRSYS KSELSLSNHS SSNTVFGTKR MGRFLFPKKL IPKSVLNTQK 251 KKKLVIDLDE TLIHSASRST THSNSSQGHL VEVKFGLSGI RTLYFIHKRP 301 YCDLFLTKVS KWYDLIIFTA SMKEYADPVI DWLESSFPSS FSKRYYRSDC 351 VLRDGVGYIK DLSIVKDSEE NGKGSSSSLD DVIIIDNSPV SYAMNVDNAI 401 QVEGWISDPT DTDLLNLLPF LEAMRYSTDV RNILALKHGE KAFNIN*

Amino Acid Sequence (or in FASTA format)

       1  MNALKYFSNH LITTKKQKKI NVEVTKNQDL LGPSKEVSNK YTSHSENDCV
      51  SEVDQQYDHS SSHLKESDQN QERKNSVPKK PKALRSILIE KIASILWALL
     101  LFLPYYLIIK PLMSLWFVFT FPLSVIERRV KHTDKRNRGS NASENELPVS
     151  SSNINDSSEK TNPKNCNLNT IPEAVEDDLN ASDEIILQRD NVKGSLLRAQ
     201  SVKSRPRSYS KSELSLSNHS SSNTVFGTKR MGRFLFPKKL IPKSVLNTQK
     251  KKKLVIDLDE TLIHSASRST THSNSSQGHL VEVKFGLSGI RTLYFIHKRP
     301  YCDLFLTKVS KWYDLIIFTA SMKEYADPVI DWLESSFPSS FSKRYYRSDC
     351  VLRDGVGYIK DLSIVKDSEE NGKGSSSSLD DVIIIDNSPV SYAMNVDNAI
     401  QVEGWISDPT DTDLLNLLPF LEAMRYSTDV RNILALKHGE KAFNIN*

external links for Nem1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Nem1p

1)	Siniossoglou S, et al. (1998) A novel complex of membrane proteins required for formation of a spherical nucleus. EMBO J 17(22):6449-64
2)	Santos-Rosa H, et al. (2005) The yeast lipin Smp2 couples phospholipid biosynthesis to nuclear membrane growth. EMBO J 24(11):1931-41
3)	Kim Y, et al. (2007) A conserved phosphatase cascade that regulates nuclear membrane biogenesis. Proc Natl Acad Sci U S A 104(16):6596-601
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41