NEM1/YHR004C Protein Information Help

Standard Name Nem1p 1
Systematic Name Yhr004cp
ORF Classification Verified
Description Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard (1, 2, 3)
Name Description Nuclear Envelope Morphology 1
Experimental Data
Molecules/cell 377 4
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 446
Molecular Weight (Da) 50,641
Isoelectric Point (pI) 9.62

Click on image for expanded interactive view
pbrowse

Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Nem1p (InterPro)
Transmembrane Domains There are 1 total predicted transmembrane domains (TMHMM)
Physical Interactions There are 5 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052E73 | P38757
MIPS: YHR004C
NCBI: 500822 | 51013351 | 6321791 | 731630 | NP_011867.1 | NM_001179134.1
GenBank/EMBL/DDBJ: AAB68431.1 | AAT92969.1 | DAA06691.1 | AY692950 | U10555
External Classifications EC: 3.1.3.16 [Protein-serine/threonine phosphatase]
Amino Acid Sequence (or in FASTA format)
       1  MNALKYFSNH LITTKKQKKI NVEVTKNQDL LGPSKEVSNK YTSHSENDCV
      51  SEVDQQYDHS SSHLKESDQN QERKNSVPKK PKALRSILIE KIASILWALL
     101  LFLPYYLIIK PLMSLWFVFT FPLSVIERRV KHTDKRNRGS NASENELPVS
     151  SSNINDSSEK TNPKNCNLNT IPEAVEDDLN ASDEIILQRD NVKGSLLRAQ
     201  SVKSRPRSYS KSELSLSNHS SSNTVFGTKR MGRFLFPKKL IPKSVLNTQK
     251  KKKLVIDLDE TLIHSASRST THSNSSQGHL VEVKFGLSGI RTLYFIHKRP
     301  YCDLFLTKVS KWYDLIIFTA SMKEYADPVI DWLESSFPSS FSKRYYRSDC
     351  VLRDGVGYIK DLSIVKDSEE NGKGSSSSLD DVIIIDNSPV SYAMNVDNAI
     401  QVEGWISDPT DTDLLNLLPF LEAMRYSTDV RNILALKHGE KAFNIN*   

external links for Nem1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) CYC2008 (complexes) MIPS YeastGFP
YGOB Complexome Pfam domains YeastRC Public Image Repository
YOGY DIP YeastRC Structure Prediction (Seattle)

GeneMANIA


IMP


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Nem1p
1) Siniossoglou S, et al.  (1998) A novel complex of membrane proteins required for formation of a spherical nucleus. EMBO J 17(22):6449-64
2) Santos-Rosa H, et al.  (2005) The yeast lipin Smp2 couples phospholipid biosynthesis to nuclear membrane growth. EMBO J 24(11):1931-41
3) Kim Y, et al.  (2007) A conserved phosphatase cascade that regulates nuclear membrane biogenesis. Proc Natl Acad Sci U S A 104(16):6596-601
4) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41