TCD1/YHR003C Protein Information

Standard Name	Tcd1p 1
Systematic Name	Yhr003cp
ORF Classification	Verified
Description	tRNA threonylcarbamoyladenosine dehydratase; required for the ct6A tRNA base modification, where an adenosine at position 37 is modified to form a cyclized active ester with an oxazolone ring; localized to the mitochondrial outer membrane; TCD1 has a paralog, TCD2, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description	tRNA ThreonylCarbamoyladenosine Dehydratase 1

Experimental Data
Molecules/cell	8430 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	429
Molecular Weight (Da)	48,883
Isoelectric Point (pI)	6.77

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Tcd1p (InterPro)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions	There are 6 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013B235 \| P38756 MIPS: YHR003C NCBI: 500821 \| 6321790 \| 731629 \| NP_011866.1 GenBank/EMBL/DDBJ: DAA06690.1 \| U10555

Amino Acid Sequence (or in FASTA format)
1 MANNTWKLIA TTALISVFST QLAKSVWKEY KLSCAANKNK TVSRPRQYDD 51 HLFREQLARN YAFLGEEGMR KIKEQYIVIV GAGEVGSWVC TMLIRSGCQK 101 IMIIDPENIS IDSLNTHCCA VLSDIGKPKV QCLKEHLSKI APWSEIKARA 151 KAWTKENSHD LIFADGESPT FIVDCLDNLE SKVDLLEYAH HNKIDVISSM 201 GVATKSDPTR VSINDISMTE FDPISRCVRR KLRKRGIATG ISVVFSNEML 251 DPRRDDILSP IDCEHRAINA VRDEALRHLP ELGTMPGIFG LSIATWILTK 301 VSGYPMKENE VKNRLKFYDS ILETFQKQMA RLNENKERSS LLGLEEVGYI 351 VEEMFRGKSP ISGYSTKLAL TKWEANKEIS LTNVVLMTKE EQEIHEKRIL 401 LDGEKLTAVY SEEVLDFIER LFKEEEYYS*

Amino Acid Sequence (or in FASTA format)

       1  MANNTWKLIA TTALISVFST QLAKSVWKEY KLSCAANKNK TVSRPRQYDD
      51  HLFREQLARN YAFLGEEGMR KIKEQYIVIV GAGEVGSWVC TMLIRSGCQK
     101  IMIIDPENIS IDSLNTHCCA VLSDIGKPKV QCLKEHLSKI APWSEIKARA
     151  KAWTKENSHD LIFADGESPT FIVDCLDNLE SKVDLLEYAH HNKIDVISSM
     201  GVATKSDPTR VSINDISMTE FDPISRCVRR KLRKRGIATG ISVVFSNEML
     251  DPRRDDILSP IDCEHRAINA VRDEALRHLP ELGTMPGIFG LSIATWILTK
     301  VSGYPMKENE VKNRLKFYDS ILETFQKQMA RLNENKERSS LLGLEEVGYI
     351  VEEMFRGKSP ISGYSTKLAL TKWEANKEIS LTNVVLMTKE EQEIHEKRIL
     401  LDGEKLTAVY SEEVLDFIER LFKEEEYYS*

external links for Tcd1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Tcd1p

1)	Miyauchi K, et al. (2013) A cyclic form of N6-threonylcarbamoyladenosine as a widely distributed tRNA hypermodification. Nat Chem Biol 9(2):105-11
2)	Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
3)	Sickmann A, et al. (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12
4)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5)	Zahedi RP, et al. (2006) Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins. Mol Biol Cell 17(3):1436-50
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41