RPL8A/YHL033C Protein Information

Standard Name	Rpl8ap
Systematic Name	Yhl033cp
Alias	Mak7p
ORF Classification	Verified
Description	Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7)
Name Description	Ribosomal Protein of the Large subunit
Gene Product	L4A 4 , L8A 4 , L8e 7 , YL5 4 , rp6 4

Experimental Data
Molecules/cell	238000 8

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	256
Molecular Weight (Da)	28,125
Isoelectric Point (pI)	10.85

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rpl8ap (InterPro)
Physical Interactions	There are 132 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000016833C \| P17076 MIPS: YHL033C NCBI: 133017 \| 2289862 \| 315113307 \| 315113533 \| 315113578 \| 365767258 \| 365767299 \| 3871 \| 410562485 \| 4396 \| 49258845 \| 6321754 \| 747613 \| NP_011830.1 GenBank/EMBL/DDBJ: DAA06652.1 \| U11583 \| U17361 \| X17204 \| X56836

Amino Acid Sequence (or in FASTA format)
1 MAPGKKVAPA PFGAKSTKSN KTRNPLTHST PKNFGIGQAV QPKRNLSRYV 51 KWPEYVRVQR QKKILSIRLK VPPTIAQFQY TLDRNTAAET FKLFNKYRPE 101 TAAEKKERLT KEAAAVAEGK SKQDASPKPY AVKYGLNHVV ALIENKKAKL 151 VLIANDVDPI ELVVFLPALC KKMGVPYAIV KGKARLGTLV NQKTSAVAAL 201 TEVRAEDEAA LAKLVSTIDA NFADKYDEVK KHWGGGILGN KAQAKMDKRA 251 KNSDSA*

Amino Acid Sequence (or in FASTA format)

       1  MAPGKKVAPA PFGAKSTKSN KTRNPLTHST PKNFGIGQAV QPKRNLSRYV
      51  KWPEYVRVQR QKKILSIRLK VPPTIAQFQY TLDRNTAAET FKLFNKYRPE
     101  TAAEKKERLT KEAAAVAEGK SKQDASPKPY AVKYGLNHVV ALIENKKAKL
     151  VLIANDVDPI ELVVFLPALC KKMGVPYAIV KGKARLGTLV NQKTSAVAAL
     201  TEVRAEDEAA LAKLVSTIDA NFADKYDEVK KHWGGGILGN KAQAKMDKRA
     251  KNSDSA*

external links for Rpl8ap

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Rpl8ap

1)	Ohtake Y and Wickner RB (1995) Yeast virus propagation depends critically on free 60S ribosomal subunit concentration. Mol Cell Biol 15(5):2772-81
2)	Yon J, et al. (1991) The organization and expression of the Saccharomyces cerevisiae L4 ribosomal protein genes and their identification as the homologues of the mammalian ribosomal protein gene L7a. Mol Gen Genet 227(1):72-80
3)	Arevalo SG and Warner JR (1990) Ribosomal protein L4 of Saccharomyces cerevisiae: the gene and its protein. Nucleic Acids Res 18(6):1447-9
4)	Planta RJ and Mager WH (1998) The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae. Yeast 14(5):471-7
5)	Lecompte O, et al. (2002) Comparative analysis of ribosomal proteins in complete genomes: an example of reductive evolution at the domain scale. Nucleic Acids Res 30(24):5382-90
6)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7)	Jenner L, et al. (2012) Crystal structure of the 80S yeast ribosome. Curr Opin Struct Biol 22(6):759-67
8)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41