ECM29/YHL030W Protein Information

Standard Name	Ecm29p 1
Systematic Name	Yhl030wp
ORF Classification	Verified
Description	Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress (2, 3, 4)
Name Description	ExtraCellular Mutant 1

Experimental Data
Molecules/cell	2950 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,868
Molecular Weight (Da)	210,429
Isoelectric Point (pI)	6.5

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ecm29p (InterPro)
Physical Interactions	There are 240 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052F28 \| P38737 MIPS: YHL030W NCBI: 2289859 \| 6321757 \| 731610 \| NP_011833.1 GenBank/EMBL/DDBJ: DAA06655.1 \| U11583

Amino Acid Sequence (or in FASTA format)
1 MSISSDEAKE KQLVEKAELR LAIADSPQKF ETNLQTFLPP LLLKLASPHA 51 SVRTAVFSAL KNLISRINTL PQVQLPVRAL IVQAKEPNLA AQQDSTNVRL 101 YSLLLASKGI DRLSLQDRQQ LLPLVVSNIS CLTGTVAARM FHILLKLILE 151 WVAPQESSHE QEEFVQFLQL DNDGFSFLMR QFTRFFLLVP SKQVQVSQQP 201 LSRGYTCPGL SLTDVAFFTY DAGVTFNKEQ LNKFKKAIFQ FVCRGMAATQ 251 TIEQSPRMIE LMEFLCVVST DSTNLSDDAA QFMKRFPMPY ENEEFITFLQ 301 TLYIGNTANG RPPVKAILQE KILSILNRSH FATTKAECIS LICSIGLHSS 351 EYKLRSLTLS FIRHVAKLNY KNLNPASSSP SSTDFSTCIV SLIRNNLHAE 401 GWPKLQLGPQ TPAFNTAILQ RQLQYETLGD ILKRDFELVS DLSYIEFLFE 451 SLKNDLPQFR SSIQESLLSL VGHLSILPQQ SKLKLKNLLR KNLSIDEQQR 501 EDNNDAVNSI MALKFVSIKF TNAAFPFHDP EARLFNIWGT VRTNRFDIIE 551 ESFKGLQPFW FRVNNASINT SATVKTSDLL GSHLSETEFP PFREFLQVLI 601 DQLDSEAASI TRKSLNNAVR FSKQCLISNA IYGKKTMVIQ DEDWSVRIDK 651 ALELDDTVVS RVNEMVQGMN DDIFIRYLTL LSNEFTATNS KGEQIAIFPY 701 QDPIFGSVLL TLLNFVSNNV LRRLEILVPD LYHLVIMKFQ SLSDNDLAVC 751 ATIIGIISTA IADSTHVKRI TKIAQSQTMA ETYVASYVVP RLYLKDQTNH 801 IESDSILNLL NILTTHLSHP GTNKDMILKL VCQVTKFGLL LQVSAQERKD 851 FLKKVMDTIQ DKLINDVTAI QTWSYLSLYS TDLENSSLFQ EKLLETNVSK 901 QNDFLFSVGE SLSVVAGKWS SKYLIKQIDI PNFNVEIMQQ KFPATNVTTI 951 LDEIFSGCDS TKPSLRKASC IWLLSYIQYL GHLPEVSSKC NDIHLRFMRF 1001 LADRDEFIQD SAARGLSLVY EIGGSDLKES MVKGLLKSFT ESTAGSASTS 1051 ATGISGSVSE ETELFEPGVL NTGDGSISTY KDILNLASEV GDPALVYKFM 1101 SLAKSSALWS SRKGIAFGLG AIMSKSSLEE LLLKDQQTAK KLIPKLYRYR 1151 FDPFQAVSRS MTDIWNTLIP ESSLTISLYF NDILDELLCG MANKEWRVRE 1201 ASTSALLQLI QSQPQEKFSE KMLKIWTMAF RTMDDIKDSV REVGTKFTTV 1251 LAKILARSID VEKGVNPTKS KEILDNILPF LWGPHGLNSD AEEVRNFALT 1301 TLIDLVKHSP GAIKPFTPKL IYDFITLFSS IEPQVINYLA LNAANYNIDA 1351 NVIDTQRKNG VTNSPLFQTI EKLINNSDDC MMEEIINVVI KASRKSVGLP 1401 SKVASSLVII ILVKRYSIEM KPYSGKLLKV CLTMFEDRNE SVNIAFAISM 1451 GYLFKVSALD KCIKYSEKLI TKYFEPTSTE NNKKVVGTAI DSILNYAKSE 1501 FDNVASVFMP LIFIACNDED KDLETLYNKI WTEASSSGAG TVKLYLPEIL 1551 NVLCVNIKSN DFSIRKTCAK SVIQLCGGIN DSIPYPQIVK LFDISREALS 1601 GRSWDGKEHI VAALVSLTEK FSQTVADNND LQESINHVMY TEVSRKSMKY 1651 VKKILPLYAR YINVNPQEET ITFLIEKAKE MIRLLGSESD DSEGSIKQTS 1701 DESTIKRIKP NTEITQKSSK ENIENEEYVI NLLKVSVDIC NNSKSRYPMN 1751 LLEFIIDEIA YLFHNDRIIH TWRTQLAASE IGISIVGRFS TISSADFIQN 1801 VGRLWDQTFP INCNKETIEN VKLQMIKFGG LIIQKIPSLQ NNIEENLRLL 1851 NSIDSTSRIE LELKNIGL*

Amino Acid Sequence (or in FASTA format)

       1  MSISSDEAKE KQLVEKAELR LAIADSPQKF ETNLQTFLPP LLLKLASPHA
      51  SVRTAVFSAL KNLISRINTL PQVQLPVRAL IVQAKEPNLA AQQDSTNVRL
     101  YSLLLASKGI DRLSLQDRQQ LLPLVVSNIS CLTGTVAARM FHILLKLILE
     151  WVAPQESSHE QEEFVQFLQL DNDGFSFLMR QFTRFFLLVP SKQVQVSQQP
     201  LSRGYTCPGL SLTDVAFFTY DAGVTFNKEQ LNKFKKAIFQ FVCRGMAATQ
     251  TIEQSPRMIE LMEFLCVVST DSTNLSDDAA QFMKRFPMPY ENEEFITFLQ
     301  TLYIGNTANG RPPVKAILQE KILSILNRSH FATTKAECIS LICSIGLHSS
     351  EYKLRSLTLS FIRHVAKLNY KNLNPASSSP SSTDFSTCIV SLIRNNLHAE
     401  GWPKLQLGPQ TPAFNTAILQ RQLQYETLGD ILKRDFELVS DLSYIEFLFE
     451  SLKNDLPQFR SSIQESLLSL VGHLSILPQQ SKLKLKNLLR KNLSIDEQQR
     501  EDNNDAVNSI MALKFVSIKF TNAAFPFHDP EARLFNIWGT VRTNRFDIIE
     551  ESFKGLQPFW FRVNNASINT SATVKTSDLL GSHLSETEFP PFREFLQVLI
     601  DQLDSEAASI TRKSLNNAVR FSKQCLISNA IYGKKTMVIQ DEDWSVRIDK
     651  ALELDDTVVS RVNEMVQGMN DDIFIRYLTL LSNEFTATNS KGEQIAIFPY
     701  QDPIFGSVLL TLLNFVSNNV LRRLEILVPD LYHLVIMKFQ SLSDNDLAVC
     751  ATIIGIISTA IADSTHVKRI TKIAQSQTMA ETYVASYVVP RLYLKDQTNH
     801  IESDSILNLL NILTTHLSHP GTNKDMILKL VCQVTKFGLL LQVSAQERKD
     851  FLKKVMDTIQ DKLINDVTAI QTWSYLSLYS TDLENSSLFQ EKLLETNVSK
     901  QNDFLFSVGE SLSVVAGKWS SKYLIKQIDI PNFNVEIMQQ KFPATNVTTI
     951  LDEIFSGCDS TKPSLRKASC IWLLSYIQYL GHLPEVSSKC NDIHLRFMRF
    1001  LADRDEFIQD SAARGLSLVY EIGGSDLKES MVKGLLKSFT ESTAGSASTS
    1051  ATGISGSVSE ETELFEPGVL NTGDGSISTY KDILNLASEV GDPALVYKFM
    1101  SLAKSSALWS SRKGIAFGLG AIMSKSSLEE LLLKDQQTAK KLIPKLYRYR
    1151  FDPFQAVSRS MTDIWNTLIP ESSLTISLYF NDILDELLCG MANKEWRVRE
    1201  ASTSALLQLI QSQPQEKFSE KMLKIWTMAF RTMDDIKDSV REVGTKFTTV
    1251  LAKILARSID VEKGVNPTKS KEILDNILPF LWGPHGLNSD AEEVRNFALT
    1301  TLIDLVKHSP GAIKPFTPKL IYDFITLFSS IEPQVINYLA LNAANYNIDA
    1351  NVIDTQRKNG VTNSPLFQTI EKLINNSDDC MMEEIINVVI KASRKSVGLP
    1401  SKVASSLVII ILVKRYSIEM KPYSGKLLKV CLTMFEDRNE SVNIAFAISM
    1451  GYLFKVSALD KCIKYSEKLI TKYFEPTSTE NNKKVVGTAI DSILNYAKSE
    1501  FDNVASVFMP LIFIACNDED KDLETLYNKI WTEASSSGAG TVKLYLPEIL
    1551  NVLCVNIKSN DFSIRKTCAK SVIQLCGGIN DSIPYPQIVK LFDISREALS
    1601  GRSWDGKEHI VAALVSLTEK FSQTVADNND LQESINHVMY TEVSRKSMKY
    1651  VKKILPLYAR YINVNPQEET ITFLIEKAKE MIRLLGSESD DSEGSIKQTS
    1701  DESTIKRIKP NTEITQKSSK ENIENEEYVI NLLKVSVDIC NNSKSRYPMN
    1751  LLEFIIDEIA YLFHNDRIIH TWRTQLAASE IGISIVGRFS TISSADFIQN
    1801  VGRLWDQTFP INCNKETIEN VKLQMIKFGG LIIQKIPSLQ NNIEENLRLL
    1851  NSIDSTSRIE LELKNIGL*

external links for Ecm29p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Ecm29p

1)	Lussier M, et al. (1997) Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae. Genetics 147(2):435-50
2)	Leggett DS, et al. (2002) Multiple associated proteins regulate proteasome structure and function. Mol Cell 10(3):495-507
3)	Lehmann A, et al. (2010) Ecm29 fulfils quality control functions in proteasome assembly. Mol Cell 38(6):879-88
4)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41