SPO11/YHL022C Protein Information

Standard Name	Spo11p 1
Systematic Name	Yhl022cp
ORF Classification	Verified
Description	Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation (2, 3)
Name Description	SPOrulation 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	398
Molecular Weight (Da)	45,412
Isoelectric Point (pI)	10

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Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Spo11p (InterPro)
Physical Interactions	There are 17 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000135D37 \| P23179 MIPS: YHL022C NCBI: 134746 \| 172660 \| 2289892 \| 45269563 \| 6321765 \| NP_011841.1 \| NM_001179102.1 GenBank/EMBL/DDBJ: DAA06663.1 \| AY557836 \| J02987 \| U11582

Amino Acid Sequence (or in FASTA format)
1 MALEGLRKKY KTRQELVKAL TPKRRSIHLN SNGHSNGTPC SNADVLAHIK 51 HFLSLAANSL EQHQQPISIV FQNKKKKGDT SSPDIHTTLD FPLNGPHLCT 101 HQFKLKRCAI LLNLLKVVME KLPLGKNTTV RDIFYSNVEL FQRQANVVQW 151 LDVIRFNFKL SPRKSLNIIP AQKGLVYSPF PIDIYDNILT CENEPKMQKQ 201 TIFPGKPCLI PFFQDDAVIK LGTTSMCNIV IVEKEAVFTK LVNNYHKLST 251 NTMLITGKGF PDFLTRLFLK KLEQYCSKLI SDCSIFTDAD PYGISIALNY 301 THSNERNAYI CTMANYKGIR ITQVLAQNNE VHNKSIQLLS LNQRDYSLAK 351 NLIASLTANS WDIATSPLKN VIIECQREIF FQKKAEMNEI DARIFEYK*

Amino Acid Sequence (or in FASTA format)

       1  MALEGLRKKY KTRQELVKAL TPKRRSIHLN SNGHSNGTPC SNADVLAHIK
      51  HFLSLAANSL EQHQQPISIV FQNKKKKGDT SSPDIHTTLD FPLNGPHLCT
     101  HQFKLKRCAI LLNLLKVVME KLPLGKNTTV RDIFYSNVEL FQRQANVVQW
     151  LDVIRFNFKL SPRKSLNIIP AQKGLVYSPF PIDIYDNILT CENEPKMQKQ
     201  TIFPGKPCLI PFFQDDAVIK LGTTSMCNIV IVEKEAVFTK LVNNYHKLST
     251  NTMLITGKGF PDFLTRLFLK KLEQYCSKLI SDCSIFTDAD PYGISIALNY
     301  THSNERNAYI CTMANYKGIR ITQVLAQNNE VHNKSIQLLS LNQRDYSLAK
     351  NLIASLTANS WDIATSPLKN VIIECQREIF FQKKAEMNEI DARIFEYK*

external links for Spo11p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Spo11p

1)	Klapholz, S. and Easton-Esposito, R. (1985) Personal Communication, Mortimer Map Edition 9
2)	Keeney S, et al. (1997) Meiosis-specific DNA double-strand breaks are catalyzed by Spo11, a member of a widely conserved protein family. Cell 88(3):375-84
3)	Lichten M (2001) Meiotic recombination: breaking the genome to save it. Curr Biol 11(7):R253-6
4)	Esposito MS, et al. (1970) Conditional mutants of meiosis in yeast. J Bacteriol 104(1):202-10