STE20/YHL007C Protein Information

Standard Name	Ste20p 1
Systematic Name	Yhl007cp
ORF Classification	Verified
Description	Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p (2, 3, 4, 5)
Name Description	STErile

Experimental Data
Molecules/cell	259 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	939
Molecular Weight (Da)	102,361
Isoelectric Point (pI)	7.33

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ste20p (InterPro)
Physical Interactions	There are 193 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013606C \| Q03497 MIPS: YHL007C NCBI: 172586 \| 172587 \| 172747 \| 294979302 \| 299688703 \| 374414440 \| 417818 \| 508679 \| 6321780 \| NP_011856.1 GenBank/EMBL/DDBJ: DAA06681.1 \| L04655 \| M94719 \| U11581
External Classifications	EC: 2.7.11.1 [Non-specific serine/threonine protein kinase] EC: 2.7.11.25 [Mitogen-activated protein kinase kinase kinase]

Amino Acid Sequence (or in FASTA format)
1 MSNDPSAVSE LPDKDSLDNG ISNDNERAMG GNGDGGDGLR LPRTTGTLNV 51 NALQKGTNAA HEAGGYKSMD PAKNAETTND DDNNVVSLDD PIQFTRVSSS 101 SVISGMSSSM SPHSNIDETK SLEAVTPNIN TSNITPDHSA DNTFSTINAS 151 ESDHQFNDTL LSKLSLTDST ETIENNATVK HQQPVASSTV NSNKSSTDIR 201 RATPVSTPVI SKPSMTTTPR QINSASHSLS NPKHKQHKPK VKPSKPEAKS 251 KPVSVKKSFP SKNPLKNSSP PKKQTEKSYY SSSSKKRKSG SNSGTLRMKD 301 VFTSFVQNIK RNSQDDKRAS SSSNNSSSSS ITTALRISTP YNAKHIHHVG 351 VDSKTGEYTG LPEEWEKLLT SSGISKREQQ QNMQAVMDIV KFYQDVTETN 401 GEDKMFKTFN TTTGLPGSPQ VSTPPANSFN KFPPSTSDSH NYGSRTGTPM 451 SNHVMSPTLN TDSSSANGKF IPSRPAPKPP SSASASAPII KSPVMNSAAN 501 VSPLKQTHAP TTPNRTSPNR SSISRNATLK KEEQPLPPIP PTKSKTSPII 551 STAHTPQQVA QSPKAPAQET VTTPTSKPAQ ARSLSKELNE KKREERERRK 601 KQLYAKLNEI CSDGDPSTKY ANLVKIGQGA SGGVYTAYEI GTNVSVAIKQ 651 MNLEKQPKKE LIINEILVMK GSKHPNIVNF IDSYVLKGDL WVIMEYMEGG 701 SLTDVVTHCI LTEGQIGAVC RETLSGLEFL HSKGVLHRDI KSDNILLSME 751 GDIKLTDFGF CAQINELNLK RTTMVGTPYW MAPEVVSRKE YGPKVDIWSL 801 GIMIIEMIEG EPPYLNETPL RALYLIATNG TPKLKEPENL SSSLKKFLDW 851 CLCVEPEDRA SATELLHDEY ITEIAEANSS LAPLVKLARL KKVAENMDAD 901 EDNDDDNDNE HINKTNNCDD NNDSKETVNL DVTEDDKQK*

Amino Acid Sequence (or in FASTA format)

       1  MSNDPSAVSE LPDKDSLDNG ISNDNERAMG GNGDGGDGLR LPRTTGTLNV
      51  NALQKGTNAA HEAGGYKSMD PAKNAETTND DDNNVVSLDD PIQFTRVSSS
     101  SVISGMSSSM SPHSNIDETK SLEAVTPNIN TSNITPDHSA DNTFSTINAS
     151  ESDHQFNDTL LSKLSLTDST ETIENNATVK HQQPVASSTV NSNKSSTDIR
     201  RATPVSTPVI SKPSMTTTPR QINSASHSLS NPKHKQHKPK VKPSKPEAKS
     251  KPVSVKKSFP SKNPLKNSSP PKKQTEKSYY SSSSKKRKSG SNSGTLRMKD
     301  VFTSFVQNIK RNSQDDKRAS SSSNNSSSSS ITTALRISTP YNAKHIHHVG
     351  VDSKTGEYTG LPEEWEKLLT SSGISKREQQ QNMQAVMDIV KFYQDVTETN
     401  GEDKMFKTFN TTTGLPGSPQ VSTPPANSFN KFPPSTSDSH NYGSRTGTPM
     451  SNHVMSPTLN TDSSSANGKF IPSRPAPKPP SSASASAPII KSPVMNSAAN
     501  VSPLKQTHAP TTPNRTSPNR SSISRNATLK KEEQPLPPIP PTKSKTSPII
     551  STAHTPQQVA QSPKAPAQET VTTPTSKPAQ ARSLSKELNE KKREERERRK
     601  KQLYAKLNEI CSDGDPSTKY ANLVKIGQGA SGGVYTAYEI GTNVSVAIKQ
     651  MNLEKQPKKE LIINEILVMK GSKHPNIVNF IDSYVLKGDL WVIMEYMEGG
     701  SLTDVVTHCI LTEGQIGAVC RETLSGLEFL HSKGVLHRDI KSDNILLSME
     751  GDIKLTDFGF CAQINELNLK RTTMVGTPYW MAPEVVSRKE YGPKVDIWSL
     801  GIMIIEMIEG EPPYLNETPL RALYLIATNG TPKLKEPENL SSSLKKFLDW
     851  CLCVEPEDRA SATELLHDEY ITEIAEANSS LAPLVKLARL KKVAENMDAD
     901  EDNDDDNDNE HINKTNNCDD NNDSKETVNL DVTEDDKQK*

external links for Ste20p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
YGOB	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YOGY	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Ste20p

1)	Leberer E, et al. (1992) The protein kinase homologue Ste20p is required to link the yeast pheromone response G-protein beta gamma subunits to downstream signalling components. EMBO J 11(13):4815-24
2)	Peter M, et al. (1996) Functional analysis of the interaction between the small GTP binding protein Cdc42 and the Ste20 protein kinase in yeast. EMBO J 15(24):7046-59
3)	Song J, et al. (2001) Molecular interactions of the Gbeta binding domain of the Ste20p/PAK family of protein kinases. An isolated but fully functional Gbeta binding domain from Ste20p is only partially folded as shown by heteronuclear NMR spectroscopy. J Biol Chem 276(44):41205-12
4)	Bartholomew CR and Hardy CF (2009) p21-activated kinases Cla4 and Ste20 regulate vacuole inheritance in Saccharomyces cerevisiae. Eukaryot Cell 8(4):560-72
5)	Lin M, et al. (2009) The Cdc42 effectors Ste20, Cla4, and Skm1 down-regulate the expression of genes involved in sterol uptake by a mitogen-activated protein kinase-independent pathway. Mol Biol Cell 20(22):4826-37
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41