YRF1-2/YER190W Protein Information

Standard Name	Yrf1-2p
Systematic Name	Yer190wp
Alias	Yrf1p
ORF Classification	Verified
Description	Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p; induced by treatment with 8-methoxypsoralen and UVA irradiation (1, 2, 3)

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,681
Molecular Weight (Da)	190,501
Isoelectric Point (pI)	7.3

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Yrf1-2p (InterPro)
Physical Interactions	There are 2 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013BFCE \| P40105 MIPS: YER190W NCBI: 2506763 \| 398365215 \| 603431 \| 971261 \| NP_011117.3 GenBank/EMBL/DDBJ: DAA07853.1 \| U18922 \| U34775
External Classifications	EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides] EC: 3.6.4.12 [DNA helicase]

Amino Acid Sequence (or in FASTA format)
1 MVYTLFQVHT LKFNRKDYDT LSLFYLNRGY YNELSFRVLE RCHEKASARP 51 NDSSTMRTFT DFVSGAPIVR SLQKSTIRKY GYNLAPYMFL LLHVDELSIF 101 SAYQASLPGE KKVDTERLKR DLCPRKPTEI KYFSQICNDM MNKKDRLGDI 151 LHIILRACAL NFGAGPRGGA GDEEDRSITN EEPIIPSVDE HGLKVCKLRS 201 PNTPRRLRKT LDAVKALLVS SCACTARDLD IFDDNNGVAM WKWIKILYHE 251 VAQETALKDS YRITLVPSSD GVSVCGKLFN REYVRGFYFA CKAQFDNLWE 301 ELNDCFYMPT VVDIASLILR NREVLFREPK RGIDEYLEND SFLQMIPVKY 351 REIVLPKLRR DTNKMTAALK NKVTVAIDEL TVPLMWMIHF AVGYPYRYPE 401 LQLLAFAGPQ RNVYVDDTTR RIQLYTDYNK NGSSEPRLKT LDGLTSDYVF 451 YFVTVLRQMQ ICALGNSYDA FNHDPWMDVV GFEDPDQVTN RDISRIVLYS 501 YMFLNTAKGC LVEYATFRQY MRELPKNAPQ KLNFREMRQG LIALGRHCVG 551 SRFETDLYES ATSELMANHS VQTGRNIYGV DSFSLTSVSG TTATLLQERA 601 SERWIQWLGL ESDYHCSFSS TRNAEDVVAG EAASSDHHQK ISRVTRKRPR 651 EPKSTNDILV AGQKLFGSSF EFRDLHQLRL CHEIYMADTP SVAVQAPPGY 701 GKTELFHLPL IALASKGDVK YVSFLFVPYT VLLANCMIRL SRCGCLNVAP 751 VRNFIEEGCD GVTDLYVGIY DDLASTNFTD RIAAWENIVE CTFRTNNVKL 801 GYLIVDEFHN FETEVYRQSQ FGGITNLDFD AFEKAIFLSG TAPEAVADAA 851 LQRIGLTGLA KKSMDINELK RSEDLSRGLS SYPTRMFNLI KEKSEVPLGH 901 VHKIWKKVES QPEEALKLLL ALFEIEPESK AIVVASTTNE VEELACSWRK 951 YFRVVWIHGK LGAAEKVSRT KEFVTDGSMR VLIGTKLVTE GIDIKQLMMV 1001 IMLDNRLNII ELIQGVGRLR DGGLCYLLSR KNSWAARNRK GELPPIKEGC 1051 ITEQVREFYG LESKKGKKGQ HVGCCGSRTD LSADTVELIE RMDRLAEKQA 1101 TASMSIVALP SSFQESNSSD RCRKYCSSDE DSDTCIHGSA NASTNATTNS 1151 STNATTTAST NVRTSATTTA SINVRTSATT TESTNSSTNA TTTASTNVRT 1201 SATTTASINV RTSATTTEST NSNTSATTTE STDSNTSATT TESTDSNTSA 1251 TTTASTNSST NATTTASTNS STNATTTEST NASAKEDANK DGNAEDNRFH 1301 PVTDINKESY KRKGSQMVLL ERKKLKAQFP NTSENMNVLQ FLGFRSDEIK 1351 HLFLYGIDVY FCPEGVFTQY GLCKGCQKMF ELCVCWAGQK VSYRRMAWEA 1401 LAVERMLRND EEYKEYLEDI EPYHGDPVGY LKYFSVKRGE IYSQIQRNYA 1451 WYLAITRRRE TISVLDSTRG KQGSQVFRMS GRQIKELYYK VWSNLRESKT 1501 EVLQYFLNWD EKKCREEWEA KDDTVFVEAL EKVGVFQRLR SMTSAGLQGP 1551 QYVKLQFSRH HRQLRSRYEL SLGMHLRDQL ALGVTPSKVP HWTAFLSMLI 1601 GLFCNKTFRQ KLEYLLEQIS EVWLLPHWLD LANVEVLAAD NTRVPLYMLM 1651 VAVHKELDSD DVPDGRFDIL LCRDSSREVG E*

Amino Acid Sequence (or in FASTA format)

       1  MVYTLFQVHT LKFNRKDYDT LSLFYLNRGY YNELSFRVLE RCHEKASARP
      51  NDSSTMRTFT DFVSGAPIVR SLQKSTIRKY GYNLAPYMFL LLHVDELSIF
     101  SAYQASLPGE KKVDTERLKR DLCPRKPTEI KYFSQICNDM MNKKDRLGDI
     151  LHIILRACAL NFGAGPRGGA GDEEDRSITN EEPIIPSVDE HGLKVCKLRS
     201  PNTPRRLRKT LDAVKALLVS SCACTARDLD IFDDNNGVAM WKWIKILYHE
     251  VAQETALKDS YRITLVPSSD GVSVCGKLFN REYVRGFYFA CKAQFDNLWE
     301  ELNDCFYMPT VVDIASLILR NREVLFREPK RGIDEYLEND SFLQMIPVKY
     351  REIVLPKLRR DTNKMTAALK NKVTVAIDEL TVPLMWMIHF AVGYPYRYPE
     401  LQLLAFAGPQ RNVYVDDTTR RIQLYTDYNK NGSSEPRLKT LDGLTSDYVF
     451  YFVTVLRQMQ ICALGNSYDA FNHDPWMDVV GFEDPDQVTN RDISRIVLYS
     501  YMFLNTAKGC LVEYATFRQY MRELPKNAPQ KLNFREMRQG LIALGRHCVG
     551  SRFETDLYES ATSELMANHS VQTGRNIYGV DSFSLTSVSG TTATLLQERA
     601  SERWIQWLGL ESDYHCSFSS TRNAEDVVAG EAASSDHHQK ISRVTRKRPR
     651  EPKSTNDILV AGQKLFGSSF EFRDLHQLRL CHEIYMADTP SVAVQAPPGY
     701  GKTELFHLPL IALASKGDVK YVSFLFVPYT VLLANCMIRL SRCGCLNVAP
     751  VRNFIEEGCD GVTDLYVGIY DDLASTNFTD RIAAWENIVE CTFRTNNVKL
     801  GYLIVDEFHN FETEVYRQSQ FGGITNLDFD AFEKAIFLSG TAPEAVADAA
     851  LQRIGLTGLA KKSMDINELK RSEDLSRGLS SYPTRMFNLI KEKSEVPLGH
     901  VHKIWKKVES QPEEALKLLL ALFEIEPESK AIVVASTTNE VEELACSWRK
     951  YFRVVWIHGK LGAAEKVSRT KEFVTDGSMR VLIGTKLVTE GIDIKQLMMV
    1001  IMLDNRLNII ELIQGVGRLR DGGLCYLLSR KNSWAARNRK GELPPIKEGC
    1051  ITEQVREFYG LESKKGKKGQ HVGCCGSRTD LSADTVELIE RMDRLAEKQA
    1101  TASMSIVALP SSFQESNSSD RCRKYCSSDE DSDTCIHGSA NASTNATTNS
    1151  STNATTTAST NVRTSATTTA SINVRTSATT TESTNSSTNA TTTASTNVRT
    1201  SATTTASINV RTSATTTEST NSNTSATTTE STDSNTSATT TESTDSNTSA
    1251  TTTASTNSST NATTTASTNS STNATTTEST NASAKEDANK DGNAEDNRFH
    1301  PVTDINKESY KRKGSQMVLL ERKKLKAQFP NTSENMNVLQ FLGFRSDEIK
    1351  HLFLYGIDVY FCPEGVFTQY GLCKGCQKMF ELCVCWAGQK VSYRRMAWEA
    1401  LAVERMLRND EEYKEYLEDI EPYHGDPVGY LKYFSVKRGE IYSQIQRNYA
    1451  WYLAITRRRE TISVLDSTRG KQGSQVFRMS GRQIKELYYK VWSNLRESKT
    1501  EVLQYFLNWD EKKCREEWEA KDDTVFVEAL EKVGVFQRLR SMTSAGLQGP
    1551  QYVKLQFSRH HRQLRSRYEL SLGMHLRDQL ALGVTPSKVP HWTAFLSMLI
    1601  GLFCNKTFRQ KLEYLLEQIS EVWLLPHWLD LANVEVLAAD NTRVPLYMLM
    1651  VAVHKELDSD DVPDGRFDIL LCRDSSREVG E*

external links for Yrf1-2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	GeneMANIA

References cited on this page View Complete Literature Guide for Yrf1-2p

1)	Yamada M, et al. (1998) Y'-Help1, a DNA helicase encoded by the yeast subtelomeric Y' element, is induced in survivors defective for telomerase. J Biol Chem 273(50):33360-6
2)	Ubersax JA, et al. (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
3)	Dardalhon M, et al. (2007) Specific transcriptional responses induced by 8-methoxypsoralen and UVA in yeast. FEMS Yeast Res 7(6):866-878