ECM32/YER176W Protein Information

Standard Name	Ecm32p 1
Systematic Name	Yer176wp
Alias	Hel1p , Mtt1p
ORF Classification	Verified
Description	DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes (2, 3, 4)
Name Description	ExtraCellular Mutant

Experimental Data
Molecules/cell	736 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,121
Molecular Weight (Da)	126,969
Isoelectric Point (pI)	10.17

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ecm32p (InterPro)
Physical Interactions	There are 1 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013AA67 \| P32644 MIPS: YER176W NCBI: 418429 \| 603417 \| 6321024 \| NP_011103.1 GenBank/EMBL/DDBJ: DAA07839.1 \| U18922
External Classifications	EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides] EC: 3.6.4.13 [RNA helicase]

Amino Acid Sequence (or in FASTA format)
1 MDFQCRTCSQ AYDAEQMMKH LSSTRHKTVF DTSNDEDICC EECQDKNIHQ 51 LQIIRFGGED MVLLCNSCFR KEYSETERPS TSYSLQNGSI LKFWEKYVKV 101 RECCCDECGE ESNLNANRNG EVLCDKCLPK SNRAKDFVSE KSGRFLYIYL 151 GLNETQNSTR KPRKKGGRRV GRGKKGRKGA KIKKEKKETF EAKISRIAYE 201 VKKENSTIQS SSSSNLRNFK GFKAVESDPV VAAKVSKSET SRSNPGPSNR 251 NKGKGNKANH KKNSGNGIGK EKERKTNIRN NVRNSQPIPE DRKNTNSHVT 301 TNSGGKGKNE SVDKHQLPQP KALNGNGSGS TNTTGLKKGK KDHAGQKTKG 351 NDKTGNKNPR EAKLNSAGRK NALGKKSNNQ PNKGTSRWTI GSDTESSREP 401 SISPNENTTS ITKSRNRNKK ASKPTLNEKS KTTTMPKKLE TKNQEKNNGK 451 TKDGKLIYEE GEPLTRYNTF KSTLSYPDLN TYLNDYSFAL FLEQKLENEF 501 VQNFNILWPR NEKDTAFIIN VEKNNNSELE KLLPANLLAL GRPAFNERQP 551 FFFCTQDEQK VWYIFIKELS IQRGKYVLLV ELFSWNNLSL PTKNGSSQFK 601 LLPTSAQTSR ILFAMTRITN PKFIDLLLGQ KPIKEIYFDN RLKFSSDKLN 651 RSQKTAVEHV LNNSITILQG PPGTGKTSTI EEIIIQVIER FHAFPILCVA 701 ASNIAIDNIA EKIMENRPQI KILRILSKKK EQQYSDDHPL GEICLHNIVY 751 KNLSPDMQVV ANKTRRGEMI SKSEDTKFYK EKNRVTNKVV SQSQIIFTTN 801 IAAGGRELKV IKECPVVIMD EATQSSEAST LVPLSLPGIR NFVFVGDEKQ 851 LSSFSNIPQL ETSLFERVLS NGTYKNPLML DTQYRMHPKI SEFPIKKIYN 901 GELKDGVTDE QKAWPGVQHP LFFYQCDLGP ESRVRSTQRD IVGFTYENKH 951 ECVEIVKIIQ ILMLDKKVPL EEIGVITPYS AQRDLLSDIL TKNVVINPKQ 1001 ISMQQEYDEI ELFNAAGSQG TAGSLQNNVI NIINGLHVAT VDSFQGHEKS 1051 FIIFSCVRNN TENKIGFLRD KRRLNVALTR AKHGLIVVGN KNVLRKGDPL 1101 WKDYITYLEE QEVIFTDLTA Y*

Amino Acid Sequence (or in FASTA format)

       1  MDFQCRTCSQ AYDAEQMMKH LSSTRHKTVF DTSNDEDICC EECQDKNIHQ
      51  LQIIRFGGED MVLLCNSCFR KEYSETERPS TSYSLQNGSI LKFWEKYVKV
     101  RECCCDECGE ESNLNANRNG EVLCDKCLPK SNRAKDFVSE KSGRFLYIYL
     151  GLNETQNSTR KPRKKGGRRV GRGKKGRKGA KIKKEKKETF EAKISRIAYE
     201  VKKENSTIQS SSSSNLRNFK GFKAVESDPV VAAKVSKSET SRSNPGPSNR
     251  NKGKGNKANH KKNSGNGIGK EKERKTNIRN NVRNSQPIPE DRKNTNSHVT
     301  TNSGGKGKNE SVDKHQLPQP KALNGNGSGS TNTTGLKKGK KDHAGQKTKG
     351  NDKTGNKNPR EAKLNSAGRK NALGKKSNNQ PNKGTSRWTI GSDTESSREP
     401  SISPNENTTS ITKSRNRNKK ASKPTLNEKS KTTTMPKKLE TKNQEKNNGK
     451  TKDGKLIYEE GEPLTRYNTF KSTLSYPDLN TYLNDYSFAL FLEQKLENEF
     501  VQNFNILWPR NEKDTAFIIN VEKNNNSELE KLLPANLLAL GRPAFNERQP
     551  FFFCTQDEQK VWYIFIKELS IQRGKYVLLV ELFSWNNLSL PTKNGSSQFK
     601  LLPTSAQTSR ILFAMTRITN PKFIDLLLGQ KPIKEIYFDN RLKFSSDKLN
     651  RSQKTAVEHV LNNSITILQG PPGTGKTSTI EEIIIQVIER FHAFPILCVA
     701  ASNIAIDNIA EKIMENRPQI KILRILSKKK EQQYSDDHPL GEICLHNIVY
     751  KNLSPDMQVV ANKTRRGEMI SKSEDTKFYK EKNRVTNKVV SQSQIIFTTN
     801  IAAGGRELKV IKECPVVIMD EATQSSEAST LVPLSLPGIR NFVFVGDEKQ
     851  LSSFSNIPQL ETSLFERVLS NGTYKNPLML DTQYRMHPKI SEFPIKKIYN
     901  GELKDGVTDE QKAWPGVQHP LFFYQCDLGP ESRVRSTQRD IVGFTYENKH
     951  ECVEIVKIIQ ILMLDKKVPL EEIGVITPYS AQRDLLSDIL TKNVVINPKQ
    1001  ISMQQEYDEI ELFNAAGSQG TAGSLQNNVI NIINGLHVAT VDSFQGHEKS
    1051  FIIFSCVRNN TENKIGFLRD KRRLNVALTR AKHGLIVVGN KNVLRKGDPL
    1101  WKDYITYLEE QEVIFTDLTA Y*

external links for Ecm32p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Ecm32p

1)	Lussier M, et al. (1997) Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae. Genetics 147(2):435-50
2)	Bean DW and Matson SW (1997) Identification of the gene encoding scHelI, a DNA helicase from Saccharomyces cerevisiae. Yeast 13(15):1465-75
3)	Biswas EE, et al. (1995) Biochemical and genetic characterization of a replication protein A dependent DNA helicase from the yeast, Saccharomyces cerevisiae. Biochem Biophys Res Commun 206(3):850-6
4)	Czaplinski K, et al. (2000) Mtt1 is a Upf1-like helicase that interacts with the translation termination factors and whose overexpression can modulate termination efficiency. RNA 6(5):730-43
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41