TMT1/YER175C Protein Information Help

Standard Name Tmt1p 1
Systematic Name Yer175cp
Alias Tam1p 2
ORF Classification Verified
Description Trans-aconitate methyltransferase; cytosolic enzyme that catalyzes the methyl esterification of 3-isopropylmalate, an intermediate of the leucine biosynthetic pathway, and trans-aconitate, which inhibits the citric acid cycle (1, 3)
Name Description Trans-aconitate MethylTransferase 1
Experimental Data
Molecules/cell 937 4
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 299
Molecular Weight (Da) 34,768
Isoelectric Point (pI) 6.2

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Tmt1p (InterPro)
Physical Interactions There are 5 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000013AA64 | P32643
MIPS: YER175C
NCBI: 224510902 | 398365095 | 418431 | 603416 | NP_011102.3 | NM_001179065.3
GenBank/EMBL/DDBJ: AAB64702.1 | DAA07837.1 | U18922
External Classifications EC: 2.1.1.145 [Trans-aconitate 3-methyltransferase]
Amino Acid Sequence (or in FASTA format)
       1  MSTFSASDFN SERYSSSRPS YPSDFYKMID EYHDGERKLL VDVGCGPGTA
      51  TLQMAQELKP FEQIIGSDLS ATMIKTAEVI KEGSPDTYKN VSFKISSSDD
     101  FKFLGADSVD KQKIDMITAV ECAHWFDFEK FQRSAYANLR KDGTIAIWGY
     151  ADPIFPDYPE FDDLMIEVPY GKQGLGPYWE QPGRSRLRNM LKDSHLDPEL
     201  FHDIQVSYFC AEDVRDKVKL HQHTKKPLLI RKQVTLVEFA DYVRTWSAYH
     251  QWKQDPKNKD KEDVADWFIK ESLRRRPELS TNTKIEVVWN TFYKLGKRV*

external links for Tmt1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) CYC2008 (complexes) MIPS YeastGFP
Candida (CGD) Complexome Pfam domains YeastRC Public Image Repository
YGOB DIP YeastRC Structure Prediction (Seattle)
YOGY GeneMANIA


IMP

References cited on this page View Complete Literature Guide for Tmt1p
1) Cai H, et al.  (2001) Identification of the gene and characterization of the activity of the trans-aconitate methyltransferase from Saccharomyces cerevisiae. Biochemistry 40(45):13699-709
2) Cai H, et al.  (2001) Distinct reactions catalyzed by bacterial and yeast trans-aconitate methyltransferases. Biochemistry 40(7):2210-9
3) Katz JE, et al.  (2004) 3-Isopropylmalate is the major endogenous substrate of the Saccharomyces cerevisiae trans-aconitate methyltransferase. Biochemistry 43(20):5976-86
4) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41