BRR2/YER172C Protein Information Help

Standard Name Brr2p 1
Systematic Name Yer172cp
Alias Rss1p 2 , Slt22p 3, 4 , Snu246p 5 , Prp44p
ORF Classification Verified
Description RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD (1, 4, 5, 6)
Name Description Bad Response to Refrigeration 1
Experimental Data
Molecules/cell 1440 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 2,163
Molecular Weight (Da) 246,183
Isoelectric Point (pI) 5.28

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Brr2p (InterPro)
Physical Interactions There are 313 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000126AE4 | P32639
MIPS: YER172C
NCBI: 240104615 | 256599941 | 256599942 | 603413 | 6321020 | 731380 | NP_011099.1
GenBank/EMBL/DDBJ: DAA07834.1 | U18922
External Classifications EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides]
Amino Acid Sequence (or in FASTA format)
 
       1  MTEHETKDKA KKIREIYRYD EMSNKVLKVD KRFMNTSQNP QRDAEISQPK
      51  SMSGRISAKD MGQGLCNNIN KGLKENDVAV EKTGKSASLK KIQQHNTILN
     101  SSSDFRLHYY PKDPSNVETY EQILQWVTEV LGNDIPHDLI IGTADIFIRQ
     151  LKENEENEDG NIEERKEKIQ HELGINIDSL KFNELVKLMK NITDYETHPD
     201  NSNKQAVAIL ADDEKSDEEE VTEMSNNANV LGGEINDNED DDEEYDYNDV
     251  EVNSKKKNKR ALPNIENDII KLSDSKTSNI ESVPIYSIDE FFLQRKLRSE
     301  LGYKDTSVIQ DLSEKILNDI ETLEHNPVAL EQKLVDLLKF ENISLAEFIL
     351  KNRSTIFWGI RLAKSTENEI PNLIEKMVAK GLNDLVEQYK FRETTHSKRE
     401  LDSGDDQPQS SEAKRTKFSN PAIPPVIDLE KIKFDESSKL MTVTKVSLPE
     451  GSFKRVKPQY DEIHIPAPSK PVIDYELKEI TSLPDWCQEA FPSSETTSLN
     501  PIQSKVFHAA FEGDSNMLIC APTGSGKTNI ALLTVLKALS HHYNPKTKKL
     551  NLSAFKIVYI APLKALVQEQ VREFQRRLAF LGIKVAELTG DSRLSRKQID
     601  ETQVLVSTPE KWDITTRNSN NLAIVELVRL LIIDEIHLLH DDRGPVLESI
     651  VARTFWASKY GQEYPRIIGL SATLPNYEDV GRFLRVPKEG LFYFDSSFRP
     701  CPLSQQFCGI KERNSLKKLK AMNDACYEKV LESINEGNQI IVFVHSRKET
     751  SRTATWLKNK FAEENITHKL TKNDAGSKQI LKTEAANVLD PSLRKLIESG
     801  IGTHHAGLTR SDRSLSEDLF ADGLLQVLVC TATLAWGVNL PAHTVIIKGT
     851  DVYSPEKGSW EQLSPQDVLQ MLGRAGRPRY DTFGEGIIIT DQSNVQYYLS
     901  VLNQQLPIES QFVSKLVDNL NAEVVAGNIK CRNDAVNWLA YTYLYVRMLA
     951  SPMLYKVPDI SSDGQLKKFR ESLVHSALCI LKEQELVLYD AENDVIEATD
    1001  LGNIASSFYI NHASMDVYNR ELDEHTTQID LFRIFSMSEE FKYVSVRYEE
    1051  KRELKQLLEK APIPIREDID DPLAKVNVLL QSYFSQLKFE GFALNSDIVF
    1101  IHQNAGRLLR AMFEICLKRG WGHPTRMLLN LCKSATTKMW PTNCPLRQFK
    1151  TCPVEVIKRL EASTVPWGDY LQLETPAEVG RAIRSEKYGK QVYDLLKRFP
    1201  KMSVTCNAQP ITRSVMRFNI EIIADWIWDM NVHGSLEPFL LMLEDTDGDS
    1251  ILYYDVLFIT PDIVGHEFTL SFTYELKQHN QNNLPPNFFL TLISENWWHS
    1301  EFEIPVSFNG FKLPKKFPPP TPLLENISIS TSELGNDDFS EVFEFKTFNK
    1351  IQSQVFESLY NSNDSVFVGS GKGTGKTAMA ELALLNHWRQ NKGRAVYINP
    1401  SGEKIDFLLS DWNKRFSHLA GGKIINKLGN DPSLNLKLLA KSHVLLATPV
    1451  QFELLSRRWR QRKNIQSLEL MIYDDAHEIS QGVYGAVYET LISRMIFIAT
    1501  QLEKKIRFVC LSNCLANARD FGEWAGMTKS NIYNFSPSER IEPLEINIQS
    1551  FKDVEHISFN FSMLQMAFEA SAAAAGNRNS SSVFLPSRKD CMEVASAFMK
    1601  FSKAIEWDML NVEEEQIVPY IEKLTDGHLR APLKHGVGIL YKGMASNDER
    1651  IVKRLYEYGA VSVLLISKDC SAFACKTDEV IILGTNLYDG AEHKYMPYTI
    1701  NELLEMVGLA SGNDSMAGKV LILTSHNMKA YYKKFLIEPL PTESYLQYII
    1751  HDTLNNEIAN SIIQSKQDCV DWFTYSYFYR RIHVNPSYYG VRDTSPHGIS
    1801  VFLSNLVETC LNDLVESSFI EIDDTEAEVT AEVNGGDDEA TEIISTLSNG
    1851  LIASHYGVSF FTIQSFVSSL SNTSTLKNML YVLSTAVEFE SVPLRKGDRA
    1901  LLVKLSKRLP LRFPEHTSSG SVSFKVFLLL QAYFSRLELP VDFQNDLKDI
    1951  LEKVVPLINV VVDILSANGY LNATTAMDLA QMLIQGVWDV DNPLRQIPHF
    2001  NNKILEKCKE INVETVYDIM ALEDEERDEI LTLTDSQLAQ VAAFVNNYPN
    2051  VELTYSLNNS DSLISGVKQK ITIQLTRDVE PENLQVTSEK YPFDKLESWW
    2101  LVLGEVSKKE LYAIKKVTLN KETQQYELEF DTPTSGKHNL TIWCVCDSYL
    2151  DADKELSFEI NVK*

external links for Brr2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
YGOB BioPIXIE MIPS YeastGFP
YOGY CYC2008 (complexes) Pfam domains YeastRC Public Image Repository

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA


YeastRC Mass Spec (Seattle)


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Brr2p
1) Noble SM and Guthrie C  (1996) Identification of novel genes required for yeast pre-mRNA splicing by means of cold-sensitive mutations. Genetics 143(1):67-80
2) Lin J and Rossi JJ  (1996) Identification and characterization of yeast mutants that overcome an experimentally introduced block to splicing at the 3' splice site. RNA 2(8):835-48
3) Xu D, et al.  (1998) Synthetic lethality of yeast slt mutations with U2 small nuclear RNA mutations suggests functional interactions between U2 and U5 snRNPs that are important for both steps of pre-mRNA splicing. Mol Cell Biol 18(4):2055-66
4) Xu D, et al.  (1996) An RNA-dependent ATPase associated with U2/U6 snRNAs in pre-mRNA splicing. Nature 381(6584):709-13
5) Lauber J, et al.  (1996) The HeLa 200 kDa U5 snRNP-specific protein and its homologue in Saccharomyces cerevisiae are members of the DEXH-box protein family of putative RNA helicases. EMBO J 15(15):4001-15
6) Raghunathan PL and Guthrie C  (1998) RNA unwinding in U4/U6 snRNPs requires ATP hydrolysis and the DEIH-box splicing factor Brr2. Curr Biol 8(15):847-55
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41