BEM2/YER155C Protein Information

Standard Name	Bem2p 1, 2
Systematic Name	Yer155cp
Alias	Ipl2p 3 , Sup9p , Tsl1p
ORF Classification	Verified
Description	Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence (4, 5)
Name Description	Bud EMergence 2

Experimental Data
Molecules/cell	1230 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	2,167
Molecular Weight (Da)	245,428
Isoelectric Point (pI)	8.6

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Bem2p (InterPro)
Physical Interactions	There are 68 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012688F \| P39960 MIPS: YER155C NCBI: 398364959 \| 499695 \| 511137 \| 603395 \| 728959 \| NP_011082.3 \| NM_001179045.3 GenBank/EMBL/DDBJ: DAA07816.1 \| L33832 \| U18917 \| Z35159

Amino Acid Sequence (or in FASTA format)
1 MKGLLWSKNR KSSTASASSS STSTSHKTTT ASTASSSSPS SSSQTIRNST 51 SGASPYMHSH HHHGQGHSHH RGEDNNRDKR KSSVFPPSKQ YTSTSSSQVN 101 LGMYHSDTNT RSSRSIASTL KDDSPSVCSE DEISNSSSQK SNAQDETPIA 151 YKKSAHSKDS LLPSRSSSLS PPQSRCSTGT TLEKSLNTSG ISNSSGTNNN 201 NSNNNNDNEQ KQRNVIHLNS ENYDTTVFKT GWVNKSHGQT VATNYNSSMT 251 APSSSSSSSS QNLRNDAYSR NRESRFYGND GSSLKNDDSS STTATNSGND 301 VASARSSMAI DPQMLVPDYR LYRAQLKGCV LNLYKSGLNS NIKFFDPTLP 351 ASNSSIANEN HQQKKQQTNN QAQAEALHQK QSFGQMGEPI TLDLKYLSEV 401 YPHPDLRQDS DGKIISGTIE SLCHTVLFYP GPKQSDVPNE KSLSKTHRAV 451 INLLLMFPLL DHFIKFLKVF NQFGLSFTKN KSRLTNNSTQ FYNISPAVDD 501 SMTQRLALTA KTILDVFPGF LLDEPMLKTI ISLLDTISLH NDEISNNLKI 551 KIANKHNELM KLTAFTRSLP MATSSTHELE IILDPSHFLS LDITTLADEV 601 HHINLKFDKV WAPKFDYSLL YDSKFINRRI VSLNPLVFNN DQNIHFLGRL 651 LISHLFPTNP EFSKKVTPKV RAELLDKWVQ IGCRFEHLGD MVSWLAVATI 701 ICSIPVLRSS SWKYVPDQSL KTIFKDWVPT IIQLERRQRT SKSTSSVFIL 751 APPNLDDDFT RANVISYFGD LLIHADDLPS DTKFKYLEKK INRTKNAFHK 801 WQQRLQAIDS TRHKTNSTEN VRDNDSPNNV VYQLWKFHLS QPPLNIEGIM 851 KLSVQHEPPI IDQKAYSTIG SQRSALVTGS YLPILFNELF PNYSLFPKNT 901 LVGAASDAKL PPPRSSARLS KSLSISEPIP IASNSHTMGS LTDDAMSSKN 951 DNNKVTGVGK IDGPVIKEMS SKQSNKQRLL KSVRDVFNID MDVFHISDEL 1001 VFKSVYDNDG KSRPASMVIE TPKRFSQHSS MLINNPATPN QKMRDSLDTT 1051 GRLSKTLENM DFFNNIGQVS DSLKESIIRV VLKSSSLEKI FDLLVLTSNI 1101 FSKLVDTKDL ENYYYHQRQR GHSTRGLSDD NIGLLDYAFV KLTMDNDIFT 1151 ETFFNTYKSF TTTTTVLENM AKRYVGAKSC SVSISKILDR SDDSKMKINE 1201 DTNLVSSSLY DQNFPVWDMK VTDDENINLI YMAKIQIGAA EAILHLVKNH 1251 YSDFTDDLCN NSTLLDIIKI MEQEVSTEWP TRIANSKLQK SLPENFVIET 1301 ENLLTTLTDL FHGIKSAYQK QLYRPIGVNR TQKRITDILN SFNTFSFTDL 1351 NNIIDDPSFS DDMIRSFQKL HSTNYEDILE WIYQLDNFIS KKFNLVSKKD 1401 WIVLFQELEL LSKESLVSFF NYPLHFKSSK LINPGYLQLH EFEISNLFTW 1451 ISTLILKDDN GTESLFFEKL PQSIKLLIKL HTSLTTFFVM EISNVNKSSS 1501 ERLTTCKVIL QILNYIRWKN GSLDLFDSEE DESPHAICPH IPAFIETAIA 1551 HAIISPESRN YELSWIKASE KLSDPTKGTQ NLRSISNVLE KIDDIHIKRF 1601 IEIDDVFSKN CKNLCPCPGW FISRLLEISQ FVPNMSITNS KLINFDKRRF 1651 VNNIISNVLD LIPNEREFPL DIEMSDENPS KRTTFGRILF NNFEDVNKVY 1701 RKKTKKVSES EAISERFQEQ GVFNEILVNE IEKIKREARK LEVLLDQEKI 1751 LKNSAALHQA VPKKNRKSVI ISGTHSDNDH SYNINKNTGQ TPSLGSVMES 1801 NNSARNRRDS RASFSTNRSS VVSNSSHNGV SKKIGGFFRR PFSIGGFNTS 1851 SSNYSLNSIL SQEVSSNKSI LPSILPEVDS MQLHDLKPSY SLKTFEIKSI 1901 MEIINHRNIP AYYYAFKIVM QNGHEYLIQT ASSSDLTEWI KMIKASKRFS 1951 FHSKKYKGKT HNKIFGVPLE DVCERENTLI PTIVVKLLEE IELRGLDEVG 2001 LYRIPGSIGS INALKNAFDE EGATDNSFTL EDDRWFEVNA IAGCFKMYLR 2051 ELPDSLFSHA MVNDFTDLAI KYKAHAMVNE EYKRMMNELL QKLPTCYYQT 2101 LKRIVFHLNK VHQHVVNNKM DASNLAIVFS MSFINQEDLA NSMGSRLGAV 2151 QTILQDFIKN PNDYFKQ*

Amino Acid Sequence (or in FASTA format)

       1  MKGLLWSKNR KSSTASASSS STSTSHKTTT ASTASSSSPS SSSQTIRNST
      51  SGASPYMHSH HHHGQGHSHH RGEDNNRDKR KSSVFPPSKQ YTSTSSSQVN
     101  LGMYHSDTNT RSSRSIASTL KDDSPSVCSE DEISNSSSQK SNAQDETPIA
     151  YKKSAHSKDS LLPSRSSSLS PPQSRCSTGT TLEKSLNTSG ISNSSGTNNN
     201  NSNNNNDNEQ KQRNVIHLNS ENYDTTVFKT GWVNKSHGQT VATNYNSSMT
     251  APSSSSSSSS QNLRNDAYSR NRESRFYGND GSSLKNDDSS STTATNSGND
     301  VASARSSMAI DPQMLVPDYR LYRAQLKGCV LNLYKSGLNS NIKFFDPTLP
     351  ASNSSIANEN HQQKKQQTNN QAQAEALHQK QSFGQMGEPI TLDLKYLSEV
     401  YPHPDLRQDS DGKIISGTIE SLCHTVLFYP GPKQSDVPNE KSLSKTHRAV
     451  INLLLMFPLL DHFIKFLKVF NQFGLSFTKN KSRLTNNSTQ FYNISPAVDD
     501  SMTQRLALTA KTILDVFPGF LLDEPMLKTI ISLLDTISLH NDEISNNLKI
     551  KIANKHNELM KLTAFTRSLP MATSSTHELE IILDPSHFLS LDITTLADEV
     601  HHINLKFDKV WAPKFDYSLL YDSKFINRRI VSLNPLVFNN DQNIHFLGRL
     651  LISHLFPTNP EFSKKVTPKV RAELLDKWVQ IGCRFEHLGD MVSWLAVATI
     701  ICSIPVLRSS SWKYVPDQSL KTIFKDWVPT IIQLERRQRT SKSTSSVFIL
     751  APPNLDDDFT RANVISYFGD LLIHADDLPS DTKFKYLEKK INRTKNAFHK
     801  WQQRLQAIDS TRHKTNSTEN VRDNDSPNNV VYQLWKFHLS QPPLNIEGIM
     851  KLSVQHEPPI IDQKAYSTIG SQRSALVTGS YLPILFNELF PNYSLFPKNT
     901  LVGAASDAKL PPPRSSARLS KSLSISEPIP IASNSHTMGS LTDDAMSSKN
     951  DNNKVTGVGK IDGPVIKEMS SKQSNKQRLL KSVRDVFNID MDVFHISDEL
    1001  VFKSVYDNDG KSRPASMVIE TPKRFSQHSS MLINNPATPN QKMRDSLDTT
    1051  GRLSKTLENM DFFNNIGQVS DSLKESIIRV VLKSSSLEKI FDLLVLTSNI
    1101  FSKLVDTKDL ENYYYHQRQR GHSTRGLSDD NIGLLDYAFV KLTMDNDIFT
    1151  ETFFNTYKSF TTTTTVLENM AKRYVGAKSC SVSISKILDR SDDSKMKINE
    1201  DTNLVSSSLY DQNFPVWDMK VTDDENINLI YMAKIQIGAA EAILHLVKNH
    1251  YSDFTDDLCN NSTLLDIIKI MEQEVSTEWP TRIANSKLQK SLPENFVIET
    1301  ENLLTTLTDL FHGIKSAYQK QLYRPIGVNR TQKRITDILN SFNTFSFTDL
    1351  NNIIDDPSFS DDMIRSFQKL HSTNYEDILE WIYQLDNFIS KKFNLVSKKD
    1401  WIVLFQELEL LSKESLVSFF NYPLHFKSSK LINPGYLQLH EFEISNLFTW
    1451  ISTLILKDDN GTESLFFEKL PQSIKLLIKL HTSLTTFFVM EISNVNKSSS
    1501  ERLTTCKVIL QILNYIRWKN GSLDLFDSEE DESPHAICPH IPAFIETAIA
    1551  HAIISPESRN YELSWIKASE KLSDPTKGTQ NLRSISNVLE KIDDIHIKRF
    1601  IEIDDVFSKN CKNLCPCPGW FISRLLEISQ FVPNMSITNS KLINFDKRRF
    1651  VNNIISNVLD LIPNEREFPL DIEMSDENPS KRTTFGRILF NNFEDVNKVY
    1701  RKKTKKVSES EAISERFQEQ GVFNEILVNE IEKIKREARK LEVLLDQEKI
    1751  LKNSAALHQA VPKKNRKSVI ISGTHSDNDH SYNINKNTGQ TPSLGSVMES
    1801  NNSARNRRDS RASFSTNRSS VVSNSSHNGV SKKIGGFFRR PFSIGGFNTS
    1851  SSNYSLNSIL SQEVSSNKSI LPSILPEVDS MQLHDLKPSY SLKTFEIKSI
    1901  MEIINHRNIP AYYYAFKIVM QNGHEYLIQT ASSSDLTEWI KMIKASKRFS
    1951  FHSKKYKGKT HNKIFGVPLE DVCERENTLI PTIVVKLLEE IELRGLDEVG
    2001  LYRIPGSIGS INALKNAFDE EGATDNSFTL EDDRWFEVNA IAGCFKMYLR
    2051  ELPDSLFSHA MVNDFTDLAI KYKAHAMVNE EYKRMMNELL QKLPTCYYQT
    2101  LKRIVFHLNK VHQHVVNNKM DASNLAIVFS MSFINQEDLA NSMGSRLGAV
    2151  QTILQDFIKN PNDYFKQ*

external links for Bem2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Bem2p

1)	Peterson J, et al. (1994) Interactions between the bud emergence proteins Bem1p and Bem2p and Rho-type GTPases in yeast. J Cell Biol 127(5):1395-406
2)	Bender A and Pringle JR (1991) Use of a screen for synthetic lethal and multicopy suppressee mutants to identify two new genes involved in morphogenesis in Saccharomyces cerevisiae. Mol Cell Biol 11(3):1295-305
3)	Chan CS and Botstein D (1993) Isolation and characterization of chromosome-gain and increase-in-ploidy mutants in yeast. Genetics 135(3):677-91
4)	Kim YJ, et al. (1994) Control of cellular morphogenesis by the Ip12/Bem2 GTPase-activating protein: possible role of protein phosphorylation. J Cell Biol 127(5):1381-94
5)	Wang T and Bretscher A (1995) The rho-GAP encoded by BEM2 regulates cytoskeletal structure in budding yeast. Mol Biol Cell 6(8):1011-24
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41