SPT15/YER148W Protein Information

Standard Name	Spt15p
Systematic Name	Yer148wp
Alias	Btf1p , Tbp1p
ORF Classification	Verified
Description	TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability (1, 2)
Name Description	SuPpressor of Ty
Gene Product	TBP

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	240
Molecular Weight (Da)	27,003
Isoelectric Point (pI)	10.16

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Spt15p (InterPro)
Physical Interactions	There are 477 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052F66 \| P13393 MIPS: YER148W NCBI: 135643 \| 1633307 \| 172899 \| 29726664 \| 29726668 \| 29726672 \| 29726676 \| 38492530 \| 398364911 \| 494632 \| 494633 \| 5822424 \| 73535263 \| 999660 \| 999661 \| NP_011075.3 GenBank/EMBL/DDBJ: DAA07809.1 \| M26403 \| M27135 \| M29459 \| U18917 \| X16860

Amino Acid Sequence (or in FASTA format)
1 MADEERLKEF KEANKIVFDP NTRQVWENQN RDGTKPATTF QSEEDIKRAA 51 PESEKDTSAT SGIVPTLQNI VATVTLGCRL DLKTVALHAR NAEYNPKRFA 101 AVIMRIREPK TTALIFASGK MVVTGAKSED DSKLASRKYA RIIQKIGFAA 151 KFTDFKIQNI VGSCDVKFPI RLEGLAFSHG TFSSYEPELF PGLIYRMVKP 201 KIVLLIFVSG KIVLTGAKQR EEIYQAFEAI YPVLSEFRKM *

Amino Acid Sequence (or in FASTA format)

       1  MADEERLKEF KEANKIVFDP NTRQVWENQN RDGTKPATTF QSEEDIKRAA
      51  PESEKDTSAT SGIVPTLQNI VATVTLGCRL DLKTVALHAR NAEYNPKRFA
     101  AVIMRIREPK TTALIFASGK MVVTGAKSED DSKLASRKYA RIIQKIGFAA
     151  KFTDFKIQNI VGSCDVKFPI RLEGLAFSHG TFSSYEPELF PGLIYRMVKP
     201  KIVLLIFVSG KIVLTGAKQR EEIYQAFEAI YPVLSEFRKM *

external links for Spt15p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Spt15p

1)	Roberts SM and Winston F (1996) SPT20/ADA5 encodes a novel protein functionally related to the TATA-binding protein and important for transcription in Saccharomyces cerevisiae. Mol Cell Biol 16(6):3206-13
2)	Yamaguchi Y, et al. (2001) SPT genes: key players in the regulation of transcription, chromatin structure and other cellular processes. J Biochem 129(2):185-91