SAK1/YER129W Protein Information

Standard Name	Sak1p 1, 2
Systematic Name	Yer129wp
Alias	Pak1p 3
ORF Classification	Verified
Description	Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome; SAK1 has a paralog, TOS3, that arose from the whole genome duplication (1, 3, 4, 5, 6, 7)
Name Description	Snf1 Activating Kinase 1

Experimental Data
Molecules/cell	752 8

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,142
Molecular Weight (Da)	126,871
Isoelectric Point (pI)	9.02

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Sak1p (InterPro)
Transmembrane Domains	There are 1 total predicted transmembrane domains (TMHMM)
Physical Interactions	There are 46 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052F24 \| P38990 MIPS: YER129W NCBI: 398364785 \| 532798 \| 603368 \| 730268 \| NP_011055.3 GenBank/EMBL/DDBJ: DAA07789.1 \| U13398 \| U18916
External Classifications	EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]

Amino Acid Sequence (or in FASTA format)
1 MDRSDKKVNV EEVNVPSNLQ IELEKSGTSS SVSLRSPTKS SATNLAGMAE 51 GARDNASIAS SSVDSLNMLL ERQRVRQLNH PQHQQHISSS LAKTPTTTSS 101 FCSSGSSKNK VKETNRISLT YDPVSKRKVL NTYEIIKELG HGQHGKVKLA 151 RDILSKQLVA IKIVDRHEKK QRKFFTFIKS SKISENDKIK REIAIMKKCH 201 HKHVVQLIEV LDDLKSRKIY LVLEYCSRGE VKWCPPDCME SDAKGPSLLS 251 FQETREILRG VVLGLEYLHY QGIIHRDIKP ANLLISGDGT VKISDFGVSL 301 AASSTNSSDS SESLDELELA KTVGTPAFFA PEMCLGEDAF TRYNLTKENL 351 FRGSCISFMI DIWAVGVTLY CLLFGMLPFF SDFELKLFEK IVNDPLKFPT 401 FKEIQSNKVS KVSCEEEYEM AKDLLLKLLE KNPQKRMTIP AIKKHPFVSW 451 DFDHVPENDE KLLSSVLEQK LRFQCNQTDQ FEPISISKHE LKNAVSGVGK 501 KIKESVLKSI PLKDPSDLSN KNYLHPTETT RGRGDANVIV SEGSVLSNIK 551 ELSANDGCLN TDSDTNININ DDDHYSGDDN DGHLTKRELE RELNKFDDKH 601 EAGNMVNLPI NSSFASLDSF YIDNFAMARM GMSSPEAGDS VSSVPNLPSA 651 PSSTRLGRSP VFSGVTNQPS PIRPVLPQQK SSFCATGRYD KSHNSLLRNS 701 SSHLTSYNSG RPSSRTGRMN SRNQNLPKIP NSLSKISTTK LTELRVPKDS 751 EIPSPAKNPN ADRLRRFPVK KNTKTPAIKD PPRININSSD KSGSKNSPIK 801 SLYQRMKQSK DNSKTFEVRR GNFFSHFNGD DDDSSSQSSV TSSGSESDSE 851 LSSTSSSCTS GTQSRNSSNN NAYSETESLP FEFGVDSEDG SGVLLRDLPN 901 EDQIRPFLDI QPCRRMKVKS SLNLEPPSVS SSSSSSSDED ELILNVGTAG 951 HRRRHNSSKL SELSNSPQKG SNNFMYSNGS VHDSETTITP QNMDDLTLHQ 1001 ALSRSQPISK PGPLVLPKRL DQKKATTETS NLTDIVEFNG NNDHRKDKNF 1051 DKVLYSRDLL KDALSSTNAG RRRSIPSNKI RGRKDASITM STNVGNDEHA 1101 RNTSCHGDKG QENGAIKQRT HERSRSLTVA ELNEEKRRSA LP*

Amino Acid Sequence (or in FASTA format)

       1  MDRSDKKVNV EEVNVPSNLQ IELEKSGTSS SVSLRSPTKS SATNLAGMAE
      51  GARDNASIAS SSVDSLNMLL ERQRVRQLNH PQHQQHISSS LAKTPTTTSS
     101  FCSSGSSKNK VKETNRISLT YDPVSKRKVL NTYEIIKELG HGQHGKVKLA
     151  RDILSKQLVA IKIVDRHEKK QRKFFTFIKS SKISENDKIK REIAIMKKCH
     201  HKHVVQLIEV LDDLKSRKIY LVLEYCSRGE VKWCPPDCME SDAKGPSLLS
     251  FQETREILRG VVLGLEYLHY QGIIHRDIKP ANLLISGDGT VKISDFGVSL
     301  AASSTNSSDS SESLDELELA KTVGTPAFFA PEMCLGEDAF TRYNLTKENL
     351  FRGSCISFMI DIWAVGVTLY CLLFGMLPFF SDFELKLFEK IVNDPLKFPT
     401  FKEIQSNKVS KVSCEEEYEM AKDLLLKLLE KNPQKRMTIP AIKKHPFVSW
     451  DFDHVPENDE KLLSSVLEQK LRFQCNQTDQ FEPISISKHE LKNAVSGVGK
     501  KIKESVLKSI PLKDPSDLSN KNYLHPTETT RGRGDANVIV SEGSVLSNIK
     551  ELSANDGCLN TDSDTNININ DDDHYSGDDN DGHLTKRELE RELNKFDDKH
     601  EAGNMVNLPI NSSFASLDSF YIDNFAMARM GMSSPEAGDS VSSVPNLPSA
     651  PSSTRLGRSP VFSGVTNQPS PIRPVLPQQK SSFCATGRYD KSHNSLLRNS
     701  SSHLTSYNSG RPSSRTGRMN SRNQNLPKIP NSLSKISTTK LTELRVPKDS
     751  EIPSPAKNPN ADRLRRFPVK KNTKTPAIKD PPRININSSD KSGSKNSPIK
     801  SLYQRMKQSK DNSKTFEVRR GNFFSHFNGD DDDSSSQSSV TSSGSESDSE
     851  LSSTSSSCTS GTQSRNSSNN NAYSETESLP FEFGVDSEDG SGVLLRDLPN
     901  EDQIRPFLDI QPCRRMKVKS SLNLEPPSVS SSSSSSSDED ELILNVGTAG
     951  HRRRHNSSKL SELSNSPQKG SNNFMYSNGS VHDSETTITP QNMDDLTLHQ
    1001  ALSRSQPISK PGPLVLPKRL DQKKATTETS NLTDIVEFNG NNDHRKDKNF
    1051  DKVLYSRDLL KDALSSTNAG RRRSIPSNKI RGRKDASITM STNVGNDEHA
    1101  RNTSCHGDKG QENGAIKQRT HERSRSLTVA ELNEEKRRSA LP*

external links for Sak1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Sak1p

1)	Elbing K, et al. (2006) Purification and characterization of the three Snf1-activating kinases of Saccharomyces cerevisiae. Biochem J 393(Pt 3):797-805
2)	Rubenstein EM, et al. (2006) Regulatory domains of Snf1-activating kinases determine pathway specificity. Eukaryot Cell 5(4):620-7
3)	Hovland PG, et al. (1997) Overexpression of the protein kinase Pak1 suppresses yeast DNA polymerase mutations. Mol Gen Genet 256(1):45-53
4)	Nath N, et al. (2003) Yeast Pak1 kinase associates with and activates Snf1. Mol Cell Biol 23(11):3909-17
5)	Sutherland CM, et al. (2003) Elm1p is one of three upstream kinases for the Saccharomyces cerevisiae SNF1 complex. Curr Biol 13(15):1299-305
6)	Hong SP, et al. (2003) Activation of yeast Snf1 and mammalian AMP-activated protein kinase by upstream kinases. Proc Natl Acad Sci U S A 100(15):8839-43
7)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
8)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41