YCK3/YER123W Protein Information

Standard Name	Yck3p
Systematic Name	Yer123wp
Alias	Cki3p
ORF Classification	Verified
Description	Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway (1, 2, 3, 4)
Name Description	Yeast Casein Kinase 1

Experimental Data
Molecules/cell	1320 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	524
Molecular Weight (Da)	60,260
Isoelectric Point (pI)	9.08

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Yck3p (InterPro)
Physical Interactions	There are 17 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000127A62 \| P39962 MIPS: YER123W NCBI: 1168964 \| 7276233 \| 7677631 \| 930267 \| NP_011049.2 \| NM_001179013.1 GenBank/EMBL/DDBJ: DAA07783.1 \| U18916 \| X87108
External Classifications	EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]

Amino Acid Sequence (or in FASTA format)
1 MSQRSSQHIV GIHYAVGPKI GEGSFGVIFE GENILHSCQA QTGSKRDSSI 51 IMANEPVAIK FEPRHSDAPQ LRDEFRAYRI LNGCVGIPHA YYFGQEGMHN 101 ILIIDLLGPS LEDLFEWCGR KFSVKTTCMV AKQMIDRVRA IHDHDLIYRD 151 IKPDNFLISQ YQRISPEGKV IKSCASSSNN DPNLIYMVDF GMAKQYRDPR 201 TKQHIPYRER KSLSGTARYM SINTHFGREQ SRRDDLESLG HVFFYFLRGS 251 LPWQGLKAPN NKLKYEKIGM TKQKLNPDDL LLNNAIPYQF ATYLKYARSL 301 KFDEDPDYDY LISLMDDALR LNDLKDDGHY DWMDLNGGKG WNIKINRRAN 351 LHGYGNPNPR VNGNTARNNV NTNSKTRNTT PVATPKQQAQ NSYNKDNSKS 401 RISSNPQSFT KQQHVLKKIE PNSKYIPETH SNLQRPIKSQ SQTYDSISHT 451 QNSPFVPYSS SKANPKRSNN EHNLPNHYTN LANKNINYQS QRNYEQENDA 501 YSDDENDTFC SKIYKYCCCC FCCC*

Amino Acid Sequence (or in FASTA format)

       1  MSQRSSQHIV GIHYAVGPKI GEGSFGVIFE GENILHSCQA QTGSKRDSSI
      51  IMANEPVAIK FEPRHSDAPQ LRDEFRAYRI LNGCVGIPHA YYFGQEGMHN
     101  ILIIDLLGPS LEDLFEWCGR KFSVKTTCMV AKQMIDRVRA IHDHDLIYRD
     151  IKPDNFLISQ YQRISPEGKV IKSCASSSNN DPNLIYMVDF GMAKQYRDPR
     201  TKQHIPYRER KSLSGTARYM SINTHFGREQ SRRDDLESLG HVFFYFLRGS
     251  LPWQGLKAPN NKLKYEKIGM TKQKLNPDDL LLNNAIPYQF ATYLKYARSL
     301  KFDEDPDYDY LISLMDDALR LNDLKDDGHY DWMDLNGGKG WNIKINRRAN
     351  LHGYGNPNPR VNGNTARNNV NTNSKTRNTT PVATPKQQAQ NSYNKDNSKS
     401  RISSNPQSFT KQQHVLKKIE PNSKYIPETH SNLQRPIKSQ SQTYDSISHT
     451  QNSPFVPYSS SKANPKRSNN EHNLPNHYTN LANKNINYQS QRNYEQENDA
     501  YSDDENDTFC SKIYKYCCCC FCCC*

external links for Yck3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Yck3p

1)	Wang X, et al. (1996) Prenylated isoforms of yeast casein kinase I, including the novel Yck3p, suppress the gcs1 blockage of cell proliferation from stationary phase. Mol Cell Biol 16(10):5375-85
2)	Sun B, et al. (2004) The yeast casein kinase Yck3p is palmitoylated, then sorted to the vacuolar membrane with AP-3-dependent recognition of a YXXPhi adaptin sorting signal. Mol Biol Cell 15(3):1397-406
3)	Lagrassa TJ and Ungermann C (2005) The vacuolar kinase Yck3 maintains organelle fragmentation by regulating the HOPS tethering complex. J Cell Biol 168(3):401-14
4)	Anand VC, et al. (2009) Genome-wide analysis of AP-3-dependent protein transport in yeast. Mol Biol Cell 20(5):1592-604
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41