SCS2/YER120W Protein Information Help

Standard Name Scs2p 1
Systematic Name Yer120wp
ORF Classification Verified
Description Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication (2, 3, 4, 5, 6, 7)
Name Description Suppressor of Choline Sensitivity 8
Experimental Data
Molecules/cell 3500 9
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 244
Molecular Weight (Da) 26,925
Isoelectric Point (pI) 4.67

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Scs2p (InterPro)
Transmembrane Domains There are 1 total predicted transmembrane domains (TMHMM)
Physical Interactions There are 76 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052E49 | P40075
MIPS: YER120W
NCBI: 398364741 | 51013701 | 603359 | 624933 | 731508 | NP_011046.3 | NM_001179010.3
GenBank/EMBL/DDBJ: DAA07780.1 | AY693125 | D44493 | U18916
Amino Acid Sequence (or in FASTA format)
 
       1  MSAVEISPDV LVYKSPLTEQ STEYASISNN SDQTIAFKVK TTAPKFYCVR
      51  PNAAVVAPGE TIQVQVIFLG LTEEPAADFK CRDKFLVITL PSPYDLNGKA
     101  VADVWSDLEA EFKQQAISKK IKVKYLISPD VHPAQNQNIQ ENKETVEPVV
     151  QDSEPKEVPA VVNEKEVPAE PETQPPVQVK KEEVPPVVQK TVPHENEKQT
     201  SNSTPAPQNQ IKEAATVPAE NESSSMGIFI LVALLILVLG WFYR*

external links for Scs2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
YGOB BioPIXIE MIPS YeastRC Public Image Repository
YOGY CYC2008 (complexes) Pfam domains

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Scs2p
1) Nikawa J, et al.  (1995) Cloning and sequence of the SCS2 gene, which can suppress the defect of INO1 expression in an inositol auxotrophic mutant of Saccharomyces cerevisiae. J Biochem 118(1):39-45
2) Kagiwada S, et al.  (1998) The Saccharomyces cerevisiae SCS2 gene product, a homolog of a synaptobrevin-associated protein, is an integral membrane protein of the endoplasmic reticulum and is required for inositol metabolism. J Bacteriol 180(7):1700-8
3) Craven RJ and Petes TD  (2001) The Saccharomyces cerevisiae suppressor of choline sensitivity (SCS2) gene is a multicopy Suppressor of mec1 telomeric silencing defects. Genetics 158(1):145-54
4) Kagiwada S and Zen R  (2003) Role of the yeast VAP homolog, Scs2p, in INO1 expression and phospholipid metabolism. J Biochem 133(4):515-22
5) Loewen CJ and Levine TP  (2005) A highly conserved binding site in vesicle-associated membrane protein-associated protein (VAP) for the FFAT motif of lipid-binding proteins. J Biol Chem 280(14):14097-104
6) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7) Manford AG, et al.  (2012) ER-to-Plasma Membrane Tethering Proteins Regulate Cell Signaling and ER Morphology. Dev Cell 23(6):1129-40
8) Hosaka K, et al.  (1994) Cloning and characterization of the SCS1 gene required for the expression of genes in yeast phospholipid synthesis. J Biochem 115(1):131-6
9) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41