SWI4/YER111C Protein Information

Standard Name	Swi4p 1
Systematic Name	Yer111cp
Alias	Art1p
ORF Classification	Verified
Description	DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p (2, 3, 4, 5, 6, 7, 8, 9)
Name Description	SWItching deficient 1

Experimental Data
Molecules/cell	589 10

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,093
Molecular Weight (Da)	123,805
Isoelectric Point (pI)	9.42

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Swi4p (InterPro)
Physical Interactions	There are 51 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013629F \| P25302 MIPS: YER111C NCBI: 603350 \| 6320957 \| 666106 \| 730855 \| NP_011036.1 GenBank/EMBL/DDBJ: DAA07771.1 \| U18916 \| X51606

Amino Acid Sequence (or in FASTA format)
1 MPFDVLISNQ KDNTNHQNIT PISKSVLLAP HSNHPVIEIA TYSETDVYEC 51 YIRGFETKIV MRRTKDDWIN ITQVFKIAQF SKTKRTKILE KESNDMQHEK 101 VQGGYGRFQG TWIPLDSAKF LVNKYEIIDP VVNSILTFQF DPNNPPPKRS 151 KNSILRKTSP GTKITSPSSY NKTPRKKNSS SSTSATTTAA NKKGKKNASI 201 NQPNPSPLQN LVFQTPQQFQ VNSSMNIMNN NDNHTTMNFN NDTRHNLINN 251 ISNNSNQSTI IQQQKSIHEN SFNNNYSATQ KPLQFFPIPT NLQNKNVALN 301 NPNNNDSNSY SHNIDNVINS SNNNNNGNNN NLIIVPDGPM QSQQQQQHHH 351 EYLTNNFNHS MMDSITNGNS KKRRKKLNQS NEQQFYNQQE KIQRHFKLMK 401 QPLLWQSFQN PNDHHNEYCD SNGSNNNNNT VASNGSSIEV FSSNENDNSM 451 NMSSRSMTPF SAGNTSSQNK LENKMTDQEY KQTILTILSS ERSSDVDQAL 501 LATLYPAPKN FNINFEIDDQ GHTPLHWATA MANIPLIKML ITLNANALQC 551 NKLGFNCITK SIFYNNCYKE NAFDEIISIL KICLITPDVN GRLPFHYLIE 601 LSVNKSKNPM IIKSYMDSII LSLGQQDYNL LKICLNYQDN IGNTPLHLSA 651 LNLNFEVYNR LVYLGASTDI LNLDNESPAS IMNKFNTPAG GSNSRNNNTK 701 ADRKLARNLP QKNYYQQQQQ QQQPQNNVKI PKIIKTQHPD KEDSTADVNI 751 AKTDSEVNES QYLHSNQPNS TNMNTIMEDL SNINSFVTSS VIKDIKSTPS 801 KILENSPILY RRRSQSISDE KEKAKDNENQ VEKKKDPLNS VKTAMPSLES 851 PSSLLPIQMS PLGKYSKPLS QQINKLNTKV SSLQRIMGEE IKNLDNEVVE 901 TESSISNNKK RLITIAHQIE DAFDSVSNKT PINSISDLQS RIKETSSKLN 951 SEKQNFIQSL EKSQALKLAT IVQDEESKVD MNTNSSSHPE KQEDEEPIPK 1001 STSETSSPKN TKADAKFSNT VQESYDVNET LRLATELTIL QFKRRMTTLK 1051 ISEAKSKINS SVKLDKYRNL IGITIENIDS KLDDIEKDLR ANA*

Amino Acid Sequence (or in FASTA format)

       1  MPFDVLISNQ KDNTNHQNIT PISKSVLLAP HSNHPVIEIA TYSETDVYEC
      51  YIRGFETKIV MRRTKDDWIN ITQVFKIAQF SKTKRTKILE KESNDMQHEK
     101  VQGGYGRFQG TWIPLDSAKF LVNKYEIIDP VVNSILTFQF DPNNPPPKRS
     151  KNSILRKTSP GTKITSPSSY NKTPRKKNSS SSTSATTTAA NKKGKKNASI
     201  NQPNPSPLQN LVFQTPQQFQ VNSSMNIMNN NDNHTTMNFN NDTRHNLINN
     251  ISNNSNQSTI IQQQKSIHEN SFNNNYSATQ KPLQFFPIPT NLQNKNVALN
     301  NPNNNDSNSY SHNIDNVINS SNNNNNGNNN NLIIVPDGPM QSQQQQQHHH
     351  EYLTNNFNHS MMDSITNGNS KKRRKKLNQS NEQQFYNQQE KIQRHFKLMK
     401  QPLLWQSFQN PNDHHNEYCD SNGSNNNNNT VASNGSSIEV FSSNENDNSM
     451  NMSSRSMTPF SAGNTSSQNK LENKMTDQEY KQTILTILSS ERSSDVDQAL
     501  LATLYPAPKN FNINFEIDDQ GHTPLHWATA MANIPLIKML ITLNANALQC
     551  NKLGFNCITK SIFYNNCYKE NAFDEIISIL KICLITPDVN GRLPFHYLIE
     601  LSVNKSKNPM IIKSYMDSII LSLGQQDYNL LKICLNYQDN IGNTPLHLSA
     651  LNLNFEVYNR LVYLGASTDI LNLDNESPAS IMNKFNTPAG GSNSRNNNTK
     701  ADRKLARNLP QKNYYQQQQQ QQQPQNNVKI PKIIKTQHPD KEDSTADVNI
     751  AKTDSEVNES QYLHSNQPNS TNMNTIMEDL SNINSFVTSS VIKDIKSTPS
     801  KILENSPILY RRRSQSISDE KEKAKDNENQ VEKKKDPLNS VKTAMPSLES
     851  PSSLLPIQMS PLGKYSKPLS QQINKLNTKV SSLQRIMGEE IKNLDNEVVE
     901  TESSISNNKK RLITIAHQIE DAFDSVSNKT PINSISDLQS RIKETSSKLN
     951  SEKQNFIQSL EKSQALKLAT IVQDEESKVD MNTNSSSHPE KQEDEEPIPK
    1001  STSETSSPKN TKADAKFSNT VQESYDVNET LRLATELTIL QFKRRMTTLK
    1051  ISEAKSKINS SVKLDKYRNL IGITIENIDS KLDDIEKDLR ANA*

external links for Swi4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Swi4p

1)	Stern M, et al. (1984) Five SWI genes are required for expression of the HO gene in yeast. J Mol Biol 178(4):853-68
2)	Andrews BJ and Moore LA (1992) Interaction of the yeast Swi4 and Swi6 cell cycle regulatory proteins in vitro. Proc Natl Acad Sci U S A 89(24):11852-6
3)	Andrews BJ and Herskowitz I (1989) The yeast SWI4 protein contains a motif present in developmental regulators and is part of a complex involved in cell-cycle-dependent transcription. Nature 342(6251):830-3
4)	Ogas J, et al. (1991) Transcriptional activation of CLN1, CLN2, and a putative new G1 cyclin (HCS26) by SWI4, a positive regulator of G1-specific transcription. Cell 66(5):1015-26
5)	Nasmyth K and Dirick L (1991) The role of SWI4 and SWI6 in the activity of G1 cyclins in yeast. Cell 66(5):995-1013
6)	Sidorova J and Breeden L (1993) Analysis of the SWI4/SWI6 protein complex, which directs G1/S-specific transcription in Saccharomyces cerevisiae. Mol Cell Biol 13(2):1069-77
7)	Spellman PT, et al. (1998) Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization. Mol Biol Cell 9(12):3273-97
8)	Harrington LA and Andrews BJ (1996) Binding to the yeast SwI4,6-dependent cell cycle box, CACGAAA, is cell cycle regulated in vivo. Nucleic Acids Res 24(4):558-65
9)	Kaluarachchi Duffy S, et al. (2012) Exploring the yeast acetylome using functional genomics. Cell 149(4):936-48
10)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41