UBC6/YER100W Protein Information

Standard Name	Ubc6p
Systematic Name	Yer100wp
Alias	Doa2p 1, 2
ORF Classification	Verified
Description	Ubiquitin-conjugating enzyme involved in ER-associated protein degradation; located at the cytosolic side of the ER membrane; tail region contains a transmembrane segment at the C-terminus; substrate of the ubiquitin-proteasome pathway (3, 4, 5, 6)
Name Description	UBiquitin-Conjugating 3

Experimental Data
Molecules/cell	1900 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	250
Molecular Weight (Da)	28,383
Isoelectric Point (pI)	6.08

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ubc6p (InterPro)
Transmembrane Domains	There are 1 total predicted transmembrane domains (TMHMM)
Physical Interactions	There are 23 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000137965 \| P33296 MIPS: YER100W NCBI: 397581 \| 398364639 \| 464983 \| 603338 \| NP_011026.3 GenBank/EMBL/DDBJ: DAA07761.1 \| U18839 \| X73234
External Classifications	EC: 6.3.2.19 [Ubiquitin--protein ligase]

Amino Acid Sequence (or in FASTA format)
1 MATKQAHKRL TKEYKLMVEN PPPYILARPN EDNILEWHYI ITGPADTPYK 51 GGQYHGTLTF PSDYPYKPPA IRMITPNGRF KPNTRLCLSM SDYHPDTWNP 101 GWSVSTILNG LLSFMTSDEA TTGSITTSDH QKKTLARNSI SYNTFQNVRF 151 KLIFPEVVQE NVETLEKRKL DEGDAANTGD ETEDPFTKAA KEKVISLEEI 201 LDPEDRIRAE QALRQSENNS KKDGKEPNDS SSMVYIGIAI FLFLVGLFMK 251 *

Amino Acid Sequence (or in FASTA format)

       1  MATKQAHKRL TKEYKLMVEN PPPYILARPN EDNILEWHYI ITGPADTPYK
      51  GGQYHGTLTF PSDYPYKPPA IRMITPNGRF KPNTRLCLSM SDYHPDTWNP
     101  GWSVSTILNG LLSFMTSDEA TTGSITTSDH QKKTLARNSI SYNTFQNVRF
     151  KLIFPEVVQE NVETLEKRKL DEGDAANTGD ETEDPFTKAA KEKVISLEEI
     201  LDPEDRIRAE QALRQSENNS KKDGKEPNDS SSMVYIGIAI FLFLVGLFMK
     251  *

external links for Ubc6p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Ubc6p

1)	Chen P, et al. (1993) Multiple ubiquitin-conjugating enzymes participate in the in vivo degradation of the yeast MAT alpha 2 repressor. Cell 74(2):357-69
2)	Hochstrasser M and Varshavsky A (1990) In vivo degradation of a transcriptional regulator: the yeast alpha 2 repressor. Cell 61(4):697-708
3)	Sommer T and Jentsch S (1993) A protein translocation defect linked to ubiquitin conjugation at the endoplasmic reticulum. Nature 365(6442):176-9
4)	Gilon T, et al. (2000) Degradation signals recognized by the Ubc6p-Ubc7p ubiquitin-conjugating enzyme pair. Mol Cell Biol 20(19):7214-9
5)	Biederer T, et al. (1996) Degradation of subunits of the Sec61p complex, an integral component of the ER membrane, by the ubiquitin-proteasome pathway. EMBO J 15(9):2069-76
6)	Walter J, et al. (2001) Sec61p-independent degradation of the tail-anchored ER membrane protein Ubc6p. EMBO J 20(12):3124-31
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41