PTC2/YER089C Protein Information Help

Standard Name Ptc2p 1
Systematic Name Yer089cp
ORF Classification Verified
Description Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint; PTC2 has a paralog, PTC3, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7, 8)
Name Description Phosphatase Two C 1
Experimental Data
Molecules/cell 12600 9
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 464
Molecular Weight (Da) 50,388
Isoelectric Point (pI) 4.7

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Ptc2p (InterPro)
Physical Interactions There are 27 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000130FE0 | P39966
MIPS: YER089C
NCBI: 1628620 | 603327 | 6320934 | 731491 | NP_011013.1 | NM_001178980.1
GenBank/EMBL/DDBJ: DAA07750.1 | U18839 | U72498
External Classifications EC: 3.1.3.16 [Phosphoprotein phosphatase]
Amino Acid Sequence (or in FASTA format)
 
       1  MGQILSNPVI DKESHSGADS LTAFGLCAMQ GWRMSMEDSH ILEPNVLTKS
      51  DKDHIAFYGI FDGHGGAKVA EYCGNKIVEI LQEQKSFHEG NLPRALIDTF
     101  INTDVKLLQD PVMKEDHSGC TATSILVSKS QNLLVCGNAG DSRTVLATDG
     151  NAKALSYDHK PTLASEKSRI VAADGFVEMD RVNGNLALSR AIGDFEFKSN
     201  PKLGPEEQIV TCVPDILEHS LDYDRDEFVI LACDGIWDCL TSQDCVDLVH
     251  LGLREGKTLN EISSRIIDVC CAPTTEGTGI GCDNMSIVVV ALLKEGEDVA
     301  QWSDRMKSKA HRTSVRSFAD KRRRVFSYYD FSKCNDEQVF AITTKKPQDK
     351  FTRDHEAAVA SVTAADNDDP MDIDDTDADT DAENLDPSSQ SKSKTSGPID
     401  LASLEALLGA TGGVKTDSNG NKVTYTLPQS ALAQLLQTMG HDPASSHPEN
     451  DSNTDHKAGR SHLQ*

external links for Ptc2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) BioPIXIE MIPS YeastGFP
Candida (CandidaDB) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Ptc2p
1) Maeda T, et al.  (1993) Mutations in a protein tyrosine phosphatase gene (PTP2) and a protein serine/threonine phosphatase gene (PTC1) cause a synthetic growth defect in Saccharomyces cerevisiae. Mol Cell Biol 13(9):5408-17
2) Cheng A, et al.  (1999) Dephosphorylation of cyclin-dependent kinases by type 2C protein phosphatases. Genes Dev 13(22):2946-57
3) Marsolier MC, et al.  (2000) Involvement of the PP2C-like phosphatase Ptc2p in the DNA checkpoint pathways of Saccharomyces cerevisiae. Genetics 154(4):1523-32
4) Welihinda AA, et al.  (1998) Protein serine/threonine phosphatase Ptc2p negatively regulates the unfolded-protein response by dephosphorylating Ire1p kinase. Mol Cell Biol 18(4):1967-77
5) Warmka J, et al.  (2001) Ptc1, a type 2C Ser/Thr phosphatase, inactivates the HOG pathway by dephosphorylating the mitogen-activated protein kinase Hog1. Mol Cell Biol 21(1):51-60
6) Young C, et al.  (2002) Role of Ptc2 type 2C Ser/Thr phosphatase in yeast high-osmolarity glycerol pathway inactivation. Eukaryot Cell 1(6):1032-40
7) Leroy C, et al.  (2003) PP2C phosphatases Ptc2 and Ptc3 are required for DNA checkpoint inactivation after a double-strand break. Mol Cell 11(3):827-35
8) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
9) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41