ILV1/YER086W Protein Information

Standard Name	Ilv1p 1
Systematic Name	Yer086wp
Alias	Iso1p
ORF Classification	Verified
Description	Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation (2, 3, 4)
Name Description	IsoLeucine-plus-Valine requiring

Experimental Data
Molecules/cell	11900 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	576
Molecular Weight (Da)	63,831
Isoelectric Point (pI)	8.84

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ilv1p (InterPro)
Physical Interactions	There are 43 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052F9C \| P00927 MIPS: YER086W NCBI: 171752 \| 3823 \| 603324 \| 6320930 \| 730940 \| NP_011009.1 GenBank/EMBL/DDBJ: DAA07746.1 \| M36383 \| U18839 \| X01466
External Classifications	EC: 4.3.1.19 [Threonine ammonia-lyase]

Amino Acid Sequence (or in FASTA format)
1 MSATLLKQPL CTVVRQGKQS KVSGLNLLRL KAHLHRQHLS PSLIKLHSEL 51 KLDELQTDNT PDYVRLVLRS SVYDVINESP ISQGVGLSSR LNTNVILKRE 101 DLLPVFSFKL RGAYNMIAKL DDSQRNQGVI ACSAGNHAQG VAFAAKHLKI 151 PATIVMPVCT PSIKYQNVSR LGSQVVLYGN DFDEAKAECA KLAEERGLTN 201 IPPFDHPYVI AGQGTVAMEI LRQVRTANKI GAVFVPVGGG GLIAGIGAYL 251 KRVAPHIKII GVETYDAATL HNSLQRNQRT PLPVVGTFAD GTSVRMIGEE 301 TFRVAQQVVD EVVLVNTDEI CAAVKDIFED TRSIVEPSGA LSVAGMKKYI 351 STVHPEIDHT KNTYVPILSG ANMNFDRLRF VSERAVLGEG KEVFMLVTLP 401 DVPGAFKKMQ KIIHPRSVTE FSYRYNEHRH ESSSEVPKAY IYTSFSVVDR 451 EKEIKQVMQQ LNALGFEAVD ISDNELAKSH GRYLVGGASK VPNERIISFE 501 FPERPGALTR FLGGLSDSWN LTLFHYRNHG ADIGKVLAGI SVPPRENLTF 551 QKFLEDLGYT YHDETDNTVY QKFLKY*

Amino Acid Sequence (or in FASTA format)

       1  MSATLLKQPL CTVVRQGKQS KVSGLNLLRL KAHLHRQHLS PSLIKLHSEL
      51  KLDELQTDNT PDYVRLVLRS SVYDVINESP ISQGVGLSSR LNTNVILKRE
     101  DLLPVFSFKL RGAYNMIAKL DDSQRNQGVI ACSAGNHAQG VAFAAKHLKI
     151  PATIVMPVCT PSIKYQNVSR LGSQVVLYGN DFDEAKAECA KLAEERGLTN
     201  IPPFDHPYVI AGQGTVAMEI LRQVRTANKI GAVFVPVGGG GLIAGIGAYL
     251  KRVAPHIKII GVETYDAATL HNSLQRNQRT PLPVVGTFAD GTSVRMIGEE
     301  TFRVAQQVVD EVVLVNTDEI CAAVKDIFED TRSIVEPSGA LSVAGMKKYI
     351  STVHPEIDHT KNTYVPILSG ANMNFDRLRF VSERAVLGEG KEVFMLVTLP
     401  DVPGAFKKMQ KIIHPRSVTE FSYRYNEHRH ESSSEVPKAY IYTSFSVVDR
     451  EKEIKQVMQQ LNALGFEAVD ISDNELAKSH GRYLVGGASK VPNERIISFE
     501  FPERPGALTR FLGGLSDSWN LTLFHYRNHG ADIGKVLAGI SVPPRENLTF
     551  QKFLEDLGYT YHDETDNTVY QKFLKY*

external links for Ilv1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Ilv1p

1)	Thuriaux P, et al. (1971) Genetic fine structure and function of mutants at the ilv1-gene locus of Saccharomyces cervisiae. Mol Gen Genet 112(1):60-72
2)	Holmberg S and Petersen JG (1988) Regulation of isoleucine-valine biosynthesis in Saccharomyces cerevisiae. Curr Genet 13(3):207-17
3)	Moreira JM, et al. (2002) Neither Reb1p nor poly(dA*T) elements are responsible for the highly specific chromatin organization at the ILV1 promoter. J Biol Chem 277(5):3202-9
4)	KAKAR SN and WAGNER RP (1964) GENETIC AND BIOCHEMICAL ANALYSIS OF ISOLEUCINE-VALINE MUTANTS OF YEAST. Genetics 49():213-22
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41