ARG5,6/YER069W Protein Information

Standard Name	Arg5,6p
Systematic Name	Yer069wp
ORF Classification	Verified
Description	Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine (1, 2, 3, 4)
Name Description	ARGinine requiring

Experimental Data
Molecules/cell	1180 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	863
Molecular Weight (Da)	94,868
Isoelectric Point (pI)	8.4

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Arg5,6p (InterPro)
Physical Interactions	There are 9 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000125D43 \| Q01217 MIPS: YER069W NCBI: 231560 \| 385867665 \| 385867666 \| 385867667 \| 385867668 \| 385867669 \| 385867670 \| 385867671 \| 385867672 \| 385867673 \| 385867674 \| 385867675 \| 385867676 \| 385867677 \| 385867678 \| 385867679 \| 385867680 \| 385867681 \| 385867682 \| 385867683 \| 385867684 \| 385867717 \| 385867718 \| 385867719 \| 385867720 \| 385867721 \| 385867722 \| 385867723 \| 385867724 \| 5155 \| 603305 \| 6320913 \| NP_010992.1 GenBank/EMBL/DDBJ: DAA07728.1 \| U18813 \| X57017
External Classifications	EC: 1.2.1.38 [N-acetyl-gamma-glutamyl-phosphate reductase] EC: 2.7.2.8 [Acetylglutamate kinase]

Amino Acid Sequence (or in FASTA format)
1 MPSASLLVST KRLNASKFQK FVSSLNKSTI AGFASVPLRA PPSVAFTRKK 51 VGYSKRYVSS TNGFSATRST VIQLLNNIST KREVEQYLKY FTSVSQQQFA 101 VIKVGGAIIS DNLHELASCL AFLYHVGLYP IVLHGTGPQV NGRLEAQGIE 151 PDYIDGIRIT DEHTMAVVRK CFLEQNLKLV TALEQLGVRA RPITSGVFTA 201 DYLDKDKYKL VGNIKSVTKE PIEASIKAGA LPILTSLAET ASGQMLNVNA 251 DVAAGELARV FEPLKIVYLN EKGGIINGST GEKISMINLD EEYDDLMKQS 301 WVKYGTKLKI REIKELLDYL PRSSSVAIIN VQDLQKELFT DSGAGTMIRR 351 GYKLVKRSSI GEFPSADALR KALQRDAGIS SGKESVASYL RYLENSDFVS 401 YADEPLEAVA IVKKDTNVPT LDKFVCSDAA WLNNVTDNVF NVLRRDFPAL 451 QWVVSENDAN IAWHFDKSQG SYLKGGKVLF WYGIDDINTI SELVENFVKS 501 CDTASTLNSS ASSGVFANKK SARSYSTRST PRPEGVNTNP GRVALIGARG 551 YTGKNLVSLI NGHPYLEVAH VSSRELKGQK LQDYTKSEII YESLQIQDIR 601 KLEEQNAVDF WVMALPNKVC EPFVETIQSV HGKSKIIDLS ADHRFVSESD 651 WAYGLPELND RAKIANAAKI ANPGCYATGS QLTISPLTKY INGLPTVFGV 701 SGYSGAGTKP SPKNDPKFLN NNLIPYALSD HIHEREISAR IGHNVAFMPH 751 VGQWFQGISL TVSIPIKKGS LSIDEIRKLY RNFYEDEKLV HVIDDIPLVK 801 DIEGTHGVVI GGFKLNDAED RVVVCATIDN LLKGAATQCL QNINLAMGYG 851 EYAGIPENKI IGV*

Amino Acid Sequence (or in FASTA format)

       1  MPSASLLVST KRLNASKFQK FVSSLNKSTI AGFASVPLRA PPSVAFTRKK
      51  VGYSKRYVSS TNGFSATRST VIQLLNNIST KREVEQYLKY FTSVSQQQFA
     101  VIKVGGAIIS DNLHELASCL AFLYHVGLYP IVLHGTGPQV NGRLEAQGIE
     151  PDYIDGIRIT DEHTMAVVRK CFLEQNLKLV TALEQLGVRA RPITSGVFTA
     201  DYLDKDKYKL VGNIKSVTKE PIEASIKAGA LPILTSLAET ASGQMLNVNA
     251  DVAAGELARV FEPLKIVYLN EKGGIINGST GEKISMINLD EEYDDLMKQS
     301  WVKYGTKLKI REIKELLDYL PRSSSVAIIN VQDLQKELFT DSGAGTMIRR
     351  GYKLVKRSSI GEFPSADALR KALQRDAGIS SGKESVASYL RYLENSDFVS
     401  YADEPLEAVA IVKKDTNVPT LDKFVCSDAA WLNNVTDNVF NVLRRDFPAL
     451  QWVVSENDAN IAWHFDKSQG SYLKGGKVLF WYGIDDINTI SELVENFVKS
     501  CDTASTLNSS ASSGVFANKK SARSYSTRST PRPEGVNTNP GRVALIGARG
     551  YTGKNLVSLI NGHPYLEVAH VSSRELKGQK LQDYTKSEII YESLQIQDIR
     601  KLEEQNAVDF WVMALPNKVC EPFVETIQSV HGKSKIIDLS ADHRFVSESD
     651  WAYGLPELND RAKIANAAKI ANPGCYATGS QLTISPLTKY INGLPTVFGV
     701  SGYSGAGTKP SPKNDPKFLN NNLIPYALSD HIHEREISAR IGHNVAFMPH
     751  VGQWFQGISL TVSIPIKKGS LSIDEIRKLY RNFYEDEKLV HVIDDIPLVK
     801  DIEGTHGVVI GGFKLNDAED RVVVCATIDN LLKGAATQCL QNINLAMGYG
     851  EYAGIPENKI IGV*

external links for Arg5,6p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Arg5,6p

1)	Boonchird C, et al. (1991) Determination of amino acid sequences involved in the processing of the ARG5/ARG6 precursor in Saccharomyces cerevisiae. Eur J Biochem 199(2):325-35
2)	Boonchird C, et al. (1991) Characterization of the yeast ARG5,6 gene: determination of the nucleotide sequence, analysis of the control region and of ARG5,6 transcript. Mol Gen Genet 226(1-2):154-66
3)	Abadjieva A, et al. (2001) A new yeast metabolon involving at least the two first enzymes of arginine biosynthesis: acetylglutamate synthase activity requires complex formation with acetylglutamate kinase. J Biol Chem 276(46):42869-80
4)	de Cima S, et al. (2012) Insight on an arginine synthesis metabolon from the tetrameric structure of yeast acetylglutamate kinase. PLoS One 7(4):e34734
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41