MOT2/YER068W Protein Information Help

Standard Name Mot2p
Systematic Name Yer068wp
Alias Not4p , Sig1p
ORF Classification Verified
Description Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications (1, 2, 3, 4, 5, 6)
Name Description Modulator Of Transcription 7
Experimental Data
Molecules/cell 4280 8
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 587
Molecular Weight (Da) 65,354
Isoelectric Point (pI) 8.04

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Mot2p (InterPro)
Physical Interactions There are 109 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052DFE | P34909
MIPS: YER068W
NCBI: 398364501 | 401815 | 425482 | 462738 | 603304 | NP_010991.3
GenBank/EMBL/DDBJ: DAA07727.1 | L26309 | M96736 | U18813
Amino Acid Sequence (or in FASTA format)
 
       1  MMNPHVQENL QAIHNALSNF DTSFLSEDEE DYCPLCIEPM DITDKNFFPC
      51  PCGYQICQFC YNNIRQNPEL NGRCPACRRK YDDENVRYVT LSPEELKMER
     101  AKLARKEKER KHREKERKEN EYTNRKHLSG TRVIQKNLVY VVGINPPVPY
     151  EEVAPTLKSE KYFGQYGKIN KIVVNRKTPH SNNTTSEHYH HHSPGYGVYI
     201  TFGSKDDAAR CIAQVDGTYM DGRLIKAAYG TTKYCSSYLR GLPCPNPNCM
     251  FLHEPGEEAD SFNKRELHNK QQAQQQSGGT AFTRSGIHNN ISTSTAGSNT
     301  NLLSENFTGT PSPAAMRAQL HHDSHTNAGT PVLTPAPVPA GSNPWGVTQS
     351  ATPVTSINLS KNSSSINLPT LNDSLGHHTT PTTENTITST TTTTNTNATS
     401  HSHGSKKKQS LAAEEYKDPY DALGNAVDFL DARLHSLSNY QKRPISIKSN
     451  IIDEETYKKY PSLFSWDKIE ASKKSDNTLA NKLVEILAIK PIDYTASVVQ
     501  FLQSVNVGVN DNITITDNTK TPTQPIRLQT VSQQIQPPLN VSTPPPGIFG
     551  PQHKVPIQQQ QMGDTSSRNS SDLLNQLING RKIIAGN*

external links for Mot2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
YGOB BioPIXIE MIPS YeastRC Public Image Repository
YOGY CYC2008 (complexes) Pfam domains

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Mot2p
1) Bai Y, et al.  (1999) The CCR4 and CAF1 proteins of the CCR4-NOT complex are physically and functionally separated from NOT2, NOT4, and NOT5. Mol Cell Biol 19(10):6642-51
2) Liu HY, et al.  (1998) The NOT proteins are part of the CCR4 transcriptional complex and affect gene expression both positively and negatively. EMBO J 17(4):1096-106
3) Badarinarayana V, et al.  (2000) Functional interaction of CCR4-NOT proteins with TATAA-binding protein (TBP) and its associated factors in yeast. Genetics 155(3):1045-54
4) Denis CL, et al.  (2001) Genetic evidence supports a role for the yeast CCR4-NOT complex in transcriptional elongation. Genetics 158(2):627-34
5) Panasenko O, et al.  (2006) The yeast Ccr4-Not complex controls ubiquitination of the nascent-associated polypeptide (NAC-EGD) complex. J Biol Chem 281(42):31389-98
6) Mersman DP, et al.  (2009) Polyubiquitination of the demethylase Jhd2 controls histone methylation and gene expression. Genes Dev 23(8):951-62
7) Cade RM and Errede B  (1994) MOT2 encodes a negative regulator of gene expression that affects basal expression of pheromone-responsive genes in Saccharomyces cerevisiae. Mol Cell Biol 14(5):3139-49
8) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41