FCY2/YER056C Protein Information

Standard Name	Fcy2p 1
Systematic Name	Yer056cp
Alias	Bra7p 2
ORF Classification	Verified
Description	Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress (3, 4, 5)
Name Description	FluoroCYtosine resistance

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	533
Molecular Weight (Da)	58,201
Isoelectric Point (pI)	4.96

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Fcy2p (InterPro)
Transmembrane Domains	There are 12 total predicted transmembrane domains (TMHMM)
Physical Interactions	There are 9 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012A5BF \| P17064 MIPS: YER056C NCBI: 3700 \| 603292 \| 6320897 \| 729467 \| NP_010976.1 GenBank/EMBL/DDBJ: DAA07713.1 \| U18813 \| V01306 \| X51751
External Classifications	TC: 2.A.39.2

Amino Acid Sequence (or in FASTA format)
1 MLEEGNNVYE IQDLEKRSPV IGSSLENEKK VAASETFTAT SEDDQQYIVE 51 SSEATKLSWF HKFFASLNAE TKGVEPVTED EKTDDSILNA ASMWFSANMV 101 IASYALGALG PMVFGLNFGQ SVLVIIFFNI MGLIFVAFFS VFGAELGLRQ 151 MILSRYLVGN VTARIFSLIN VIACVGWGIV NTSVSAQLLN MVNEGSGHVC 201 PIWAGCLIII GGTVLVTFFG YSVIHAYEKW SWVPNFAVFL VIIAQLSRSG 251 KFKGGEWVGG ATTAGSVLSF GSSIFGFAAG WTTYAADYTV YMPKSTNKYK 301 IFFSLVAGLA FPLFFTMILG AASAMAALND PTWKAYYDKN AMGGVIYAIL 351 VPNSLNGFGQ FCCVLLALST IANNIPNMYT VALSAQALWA PLAKIPRVVW 401 TMAGNAATLG ISIPATYYFD GFMENFMDSI GYYLAIYIAI SCSEHFFYRR 451 SFSAYNIDDW DNWEHLPIGI AGTAALIVGA FGVALGMCQT YWVGEIGRLI 501 GKYGGDIGFE LGASWAFIIY NILRPLELKY FGR*

Amino Acid Sequence (or in FASTA format)

       1  MLEEGNNVYE IQDLEKRSPV IGSSLENEKK VAASETFTAT SEDDQQYIVE
      51  SSEATKLSWF HKFFASLNAE TKGVEPVTED EKTDDSILNA ASMWFSANMV
     101  IASYALGALG PMVFGLNFGQ SVLVIIFFNI MGLIFVAFFS VFGAELGLRQ
     151  MILSRYLVGN VTARIFSLIN VIACVGWGIV NTSVSAQLLN MVNEGSGHVC
     201  PIWAGCLIII GGTVLVTFFG YSVIHAYEKW SWVPNFAVFL VIIAQLSRSG
     251  KFKGGEWVGG ATTAGSVLSF GSSIFGFAAG WTTYAADYTV YMPKSTNKYK
     301  IFFSLVAGLA FPLFFTMILG AASAMAALND PTWKAYYDKN AMGGVIYAIL
     351  VPNSLNGFGQ FCCVLLALST IANNIPNMYT VALSAQALWA PLAKIPRVVW
     401  TMAGNAATLG ISIPATYYFD GFMENFMDSI GYYLAIYIAI SCSEHFFYRR
     451  SFSAYNIDDW DNWEHLPIGI AGTAALIVGA FGVALGMCQT YWVGEIGRLI
     501  GKYGGDIGFE LGASWAFIIY NILRPLELKY FGR*

external links for Fcy2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	TCDB
YGOB	DIP	YeastRC Structure Prediction (Seattle)
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Fcy2p

1)	Weber, E. (1989) Personal Communication, Mortimer Map Edition 10
2)	Guetsova ML, et al. (1997) The isolation and characterization of Saccharomyces cerevisiae mutants that constitutively express purine biosynthetic genes. Genetics 147(2):383-97
3)	Ferreira T, et al. (1997) Functional analysis of mutated purine-cytosine permease from Saccharomyces cerevisiae. A possible role of the hydrophilic segment 371-377 in the active carrier conformation. J Biol Chem 272(15):9697-702
4)	Wagner R, et al. (2001) New plasmid system to select for Saccharomyces cerevisiae purine-cytosine permease affinity mutants. J Bacteriol 183(14):4386-8
5)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76