GCD11/YER025W Protein Information

Standard Name	Gcd11p 1
Systematic Name	Yer025wp
Alias	Sui4p 2
ORF Classification	Verified
Description	Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met (3)
Name Description	General Control Derepressed 4

Experimental Data
Molecules/cell	20800 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	527
Molecular Weight (Da)	57,865
Isoelectric Point (pI)	6.77

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Gcd11p (InterPro)
Physical Interactions	There are 155 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00000530C6 \| P32481 MIPS: YER025W NCBI: 171571 \| 417179 \| 603617 \| 6320863 \| NP_010942.1 GenBank/EMBL/DDBJ: DAA07678.1 \| L04268 \| U18778

Amino Acid Sequence (or in FASTA format)
1 MSDLQDQEPS IIINGNLEPV GEPDIVEETE VVAQETQETQ DADKPKKKVA 51 FTGLEEDGET EEEKRKREFE EGGGLPEQPL NPDFSKLNPL SAEIINRQAT 101 INIGTIGHVA HGKSTVVRAI SGVQTVRFKD ELERNITIKL GYANAKIYKC 151 QEPTCPEPDC YRSFKSDKEI SPKCQRPGCP GRYKLVRHVS FVDCPGHDIL 201 MSTMLSGAAV MDAALLLIAG NESCPQPQTS EHLAAIEIMK LKHVIILQNK 251 VDLMREESAL EHQKSILKFI RGTIADGAPI VPISAQLKYN IDAVNEFIVK 301 TIPVPPRDFM ISPRLIVIRS FDVNKPGAEI EDLKGGVAGG SILNGVFKLG 351 DEIEIRPGIV TKDDKGKIQC KPIFSNIVSL FAEQNDLKFA VPGGLIGVGT 401 KVDPTLCRAD RLVGQVVGAK GHLPNIYTDI EINYFLLRRL LGVKTDGQKQ 451 AKVRKLEPNE VLMVNIGSTA TGARVVAVKA DMARLQLTSP ACTEINEKIA 501 LSRRIEKHWR LIGWATIKKG TTLEPIA*

Amino Acid Sequence (or in FASTA format)

       1  MSDLQDQEPS IIINGNLEPV GEPDIVEETE VVAQETQETQ DADKPKKKVA
      51  FTGLEEDGET EEEKRKREFE EGGGLPEQPL NPDFSKLNPL SAEIINRQAT
     101  INIGTIGHVA HGKSTVVRAI SGVQTVRFKD ELERNITIKL GYANAKIYKC
     151  QEPTCPEPDC YRSFKSDKEI SPKCQRPGCP GRYKLVRHVS FVDCPGHDIL
     201  MSTMLSGAAV MDAALLLIAG NESCPQPQTS EHLAAIEIMK LKHVIILQNK
     251  VDLMREESAL EHQKSILKFI RGTIADGAPI VPISAQLKYN IDAVNEFIVK
     301  TIPVPPRDFM ISPRLIVIRS FDVNKPGAEI EDLKGGVAGG SILNGVFKLG
     351  DEIEIRPGIV TKDDKGKIQC KPIFSNIVSL FAEQNDLKFA VPGGLIGVGT
     401  KVDPTLCRAD RLVGQVVGAK GHLPNIYTDI EINYFLLRRL LGVKTDGQKQ
     451  AKVRKLEPNE VLMVNIGSTA TGARVVAVKA DMARLQLTSP ACTEINEKIA
     501  LSRRIEKHWR LIGWATIKKG TTLEPIA*

external links for Gcd11p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Gcd11p

1)	Harashima S and Hinnebusch AG (1986) Multiple GCD genes required for repression of GCN4, a transcriptional activator of amino acid biosynthetic genes in Saccharomyces cerevisiae. Mol Cell Biol 6(11):3990-8
2)	Huang HK, et al. (1997) GTP hydrolysis controls stringent selection of the AUG start codon during translation initiation in Saccharomyces cerevisiae. Genes Dev 11(18):2396-413
3)	Laurino JP, et al. (1999) The beta subunit of eukaryotic translation initiation factor 2 binds mRNA through the lysine repeats and a region comprising the C2-C2 motif. Mol Cell Biol 19(1):173-81
4)	Lucchini G, et al. (1984) Positive regulatory interactions of the HIS4 gene of Saccharomyces cerevisiae. Mol Cell Biol 4(7):1326-33
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41