SRB4/YER022W Protein Information Help

Standard Name Srb4p 1
Systematic Name Yer022wp
Alias Med17p 2
ORF Classification Verified
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination (1, 3, 4)
Name Description Suppressor of RNA polymerase B 1
Experimental Data
Molecules/cell 3650 5
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 687
Molecular Weight (Da) 78,475
Isoelectric Point (pI) 5.08

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Srb4p (InterPro)
Physical Interactions There are 273 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000005315C | P32569
MIPS: YER022W
NCBI: 172693 | 345100885 | 345100892 | 345100899 | 410563050 | 410563057 | 410563147 | 417806 | 432969 | 459358307 | 51013783 | 603614 | 6320860 | NP_010939.1 | NM_001178913.1
GenBank/EMBL/DDBJ: DAA07675.1 | AY693166 | L12026 | U18778 | X57338
Amino Acid Sequence (or in FASTA format)
 
       1  MTTEDPDSNH LSSETGIKLA LDPNLITLAL SSNPNSSLHS PTSDEPVPES
      51  AGKADTSIRL EGDELENKTK KDNDKNLKFL KNKDSLVSNP HEIYGSMPLE
     101  QLIPIILRQR GPGFKFVDLN EKELQNEIKQ LGSDSSDGHN SEKKDTDGAD
     151  ENVQIGEDFM EVDYEDKDNP VDSRNETDHK TNENGETDDN IETVMTQEQF
     201  VKRRRDMLEH INLAMNESSL ALEFVSLLLS SVKESTGMSS MSPFLRKVVK
     251  PSSLNSDKIP YVAPTKKEYI ELDILNKGWK LQSLNESKDL LRASFNKLSS
     301  ILQNEHDYWN KIMQSISNKD VIFKIRDRTS GQKLLAIKYG YEDSGSTYKH
     351  DRGIANIRNN IESQNLDLIP HSSSVFKGTD FVHSVKKFLR VRIFTKIESE
     401  DDYILSGESV MDRDSESEEA ETKDIRKQIQ LLKKIIFEKE LMYQIKKECA
     451  LLISYGVSIE NENKVIIELP NEKFEIELLS LDDDSIVNHE QDLPKINDKR
     501  ANLMLVMLRL LLVVIFKKTL RSRISSPHGL INLNVDDDIL IIRPILGKVR
     551  FANYKLLLKK IIKDYVLDIV PGSSITETEV EREQPQENKN IDDENITKLN
     601  KEIRAFDKLL NIPRRELKIN LPLTEHKSPN LSLMLESPNY CNALIHIKFS
     651  AGTEANAVSF DTTFSDFKEV EDFLHFIVAE YIQQKKV*

external links for Srb4p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Srb4p
1) Thompson CM, et al.  (1993) A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast. Cell 73(7):1361-75
2) Bourbon HM, et al.  (2004) A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. Mol Cell 14(5):553-7
3) Kornberg RD  (2005) Mediator and the mechanism of transcriptional activation. Trends Biochem Sci 30(5):235-9
4) Kaufmann R, et al.  (2010) Infantile Cerebral and Cerebellar Atrophy Is Associated with a Mutation in the MED17 Subunit of the Transcription Preinitiation Mediator Complex. Am J Hum Genet 87(5):667-70
5) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41