AFG3/YER017C Protein Information Help

Standard Name Afg3p 1
Systematic Name Yer017cp
Alias Yta10p 2
ORF Classification Verified
Description Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging (3, 4, 5)
Name Description ATPase Family Gene 1
Experimental Data
Molecules/cell 3870 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 761
Molecular Weight (Da) 84,543
Isoelectric Point (pI) 9.94

Click on image for expanded interactive view
pbrowse

Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Afg3p (InterPro)
Transmembrane Domains There are 1 total predicted transmembrane domains (TMHMM)
Signal Peptide(s) There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions There are 26 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000125663 | P39925
MIPS: YER017C
NCBI: 531750 | 541748 | 603609 | 6320854 | 728820 | NP_010933.1
GenBank/EMBL/DDBJ: DAA07669.1 | U18778 | X76643 | X81066
External Classifications EC: 3.4.24.- [Metalloendopeptidases]
Amino Acid Sequence (or in FASTA format)
 
       1  MMMWQRYARG APRSLTSLSF GKASRISTVK PVLRSRMPVH QRLQTLSGLA
      51  TRNTIHRSTQ IRSFHISWTR LNENRPNKEG EGKNNGNKDN NSNKEDGKDK
     101  RNEFGSLSEY FRSKEFANTM FLTIGFTIIF TLLTPSSNNS GDDSNRVLTF
     151  QDFKTKYLEK GLVSKIYVVN KFLVEAELVN TKQVVSFTIG SVDIFEEQMD
     201  QIQDLLNIPP RDRIPIKYIE RSSPFTFLFP FLPTIILLGG LYFITRKINS
     251  SPPNANGGGG GGLGGMFNVG KSRAKLFNKE TDIKISFKNV AGCDEAKQEI
     301  MEFVHFLKNP GKYTKLGAKI PRGAILSGPP GTGKTLLAKA TAGEANVPFL
     351  SVSGSEFVEM FVGVGASRVR DLFTQARSMA PSIIFIDEID AIGKERGKGG
     401  ALGGANDERE ATLNQLLVEM DGFTTSDQVV VLAGTNRPDV LDNALMRPGR
     451  FDRHIQIDSP DVNGRQQIYL VHLKRLNLDP LLTDDMNNLS GKLATLTPGF
     501  TGADIANACN EAALIAARHN DPYITIHHFE QAIERVIAGL EKKTRVLSKE
     551  EKRSVAYHEA GHAVCGWFLK YADPLLKVSI IPRGQGALGY AQYLPPDQYL
     601  ISEEQFRHRM IMALGGRVSE ELHFPSVTSG AHDDFKKVTQ MANAMVTSLG
     651  MSPKIGYLSF DQNDGNFKVN KPFSNKTART IDLEVKSIVD DAHRACTELL
     701  TKNLDKVDLV AKELLRKEAI TREDMIRLLG PRPFKERNEA FEKYLDPKSN
     751  TEPPEAPAAT N*

external links for Afg3p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Afg3p
1) Guelin E, et al.  (1994) Sequence of the AFG3 gene encoding a new member of the FtsH/Yme1/Tma subfamily of the AAA-protein family. Yeast 10(10):1389-94
2) Schnall R, et al.  (1994) Identification of a set of yeast genes coding for a novel family of putative ATPases with high similarity to constituents of the 26S protease complex. Yeast 10(9):1141-55
3) Arlt H, et al.  (1996) The YTA10-12 complex, an AAA protease with chaperone-like activity in the inner membrane of mitochondria. Cell 85(6):875-85
4) Arlt H, et al.  (1998) The formation of respiratory chain complexes in mitochondria is under the proteolytic control of the m-AAA protease. EMBO J 17(16):4837-47
5) Delaney JR, et al.  (2013) Stress profiling of longevity mutants identifies Afg3 as a mitochondrial determinant of cytoplasmic mRNA translation and aging. Aging Cell 12(1):156-66
6) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41