PRP22/YER013W Protein Information

Standard Name	Prp22p 1
Systematic Name	Yer013wp
ORF Classification	Verified
Description	DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes (2, 3, 4, 5, 6)
Name Description	Pre-mRNA Processing 1

Experimental Data
Molecules/cell	907 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,145
Molecular Weight (Da)	130,010
Isoelectric Point (pI)	7.79

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Prp22p (InterPro)
Physical Interactions	There are 61 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00000531F0 \| P24384 MIPS: YER013W NCBI: 130830 \| 398364321 \| 4234 \| 603605 \| NP_010929.3 GenBank/EMBL/DDBJ: DAA07664.1 \| U18778 \| X58681
External Classifications	EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides] EC: 3.6.4.13 [RNA helicase]

Amino Acid Sequence (or in FASTA format)
1 MSDISKLIGA IVGSDDPVII EFVLNIINKS GNLQEFIRNI QKLDAGISYE 51 DSIKMYNAFL GKQEEEKVRN KVKSSPLSQK INQVLKDDVN LDDPVVTEFV 101 LSILNKSKSI TEFQEQLNLM QSGLDNETIF KIYQIASPPV MKEEVSVLPS 151 TKIPAKIEAK IEEEVQKIES LDPSPVLHKV YEGKVRNITT FGCFVQIFGT 201 RMKNCDGLVH ISEMSDQRTL DPHDVVRQGQ HIFVEVIKIQ NNGKISLSMK 251 NIDQHSGEIR KRNTESVEDR GRSNDAHTSR NMKNKIKRRA LTSPERWEIR 301 QLIASGAASI DDYPELKDEI PINTSYLTAK RDDGSIVNGN TEKVDSKLEE 351 QQRDETDEID VELNTDDGPK FLKDQQVKGA KKYEMPKITK VPRGFMNRSA 401 INGSNAIRDH REEKLRKKRE IEQQIRKQQS FDDPTKNKKD SRNEIQMLKN 451 QLIVTEWEKN RMNESISYGK RTSLPISAQR QTLPVYAMRS ELIQAVRDNQ 501 FLVIVGETGS GKTTQITQYL DEEGFSNYGM IGCTQPRRVA AVSVAKRVAE 551 EVGCKVGHDV GYTIRFEDVT GPDTRIKYMT DGMLQREALL DPEMSKYSVI 601 MLDEAHERTV ATDVLFALLK KAAIKRPELK VIVTSATLNS AKFSEYFLNC 651 PIINIPGKTF PVEVLYSQTP QMDYIEAALD CVIDIHINEG PGDILVFLTG 701 QEEIDSCCEI LYDRVKTLGD SIGELLILPV YSALPSEIQS KIFEPTPKGS 751 RKVVFATNIA ETSITIDGIY YVVDPGFAKI NIYNARAGIE QLIVSPISQA 801 QANQRKGRAG RTGPGKCYRL YTESAFYNEM LENTVPEIQR QNLSHTILML 851 KAMGINDLLK FDFMDPPPKN LMLNALTELY HLQSLDDEGK LTNLGKEMSL 901 FPMDPTLSRS LLSSVDNQCS DEIVTIISML SVQNVFYRPK DRQLEADSKK 951 AKFHHPYGDH LTLLNVYTRW QQANYSEQYC KTNFLHFRHL KRARDVKSQI 1001 SMIFKKIGLK LISCHSDPDL IRKTFVSGFF MNAAKRDSQV GYKTINGGTE 1051 VGIHPSSSLY GKEYEYVMYH SIVLTSREYM SQVTSIEPQW LLEVAPHFYK 1101 AGDAESQSRK KAKIIPLHNK FAKDQNSWRL SSIRQSRERA LGIKR*

Amino Acid Sequence (or in FASTA format)

       1  MSDISKLIGA IVGSDDPVII EFVLNIINKS GNLQEFIRNI QKLDAGISYE
      51  DSIKMYNAFL GKQEEEKVRN KVKSSPLSQK INQVLKDDVN LDDPVVTEFV
     101  LSILNKSKSI TEFQEQLNLM QSGLDNETIF KIYQIASPPV MKEEVSVLPS
     151  TKIPAKIEAK IEEEVQKIES LDPSPVLHKV YEGKVRNITT FGCFVQIFGT
     201  RMKNCDGLVH ISEMSDQRTL DPHDVVRQGQ HIFVEVIKIQ NNGKISLSMK
     251  NIDQHSGEIR KRNTESVEDR GRSNDAHTSR NMKNKIKRRA LTSPERWEIR
     301  QLIASGAASI DDYPELKDEI PINTSYLTAK RDDGSIVNGN TEKVDSKLEE
     351  QQRDETDEID VELNTDDGPK FLKDQQVKGA KKYEMPKITK VPRGFMNRSA
     401  INGSNAIRDH REEKLRKKRE IEQQIRKQQS FDDPTKNKKD SRNEIQMLKN
     451  QLIVTEWEKN RMNESISYGK RTSLPISAQR QTLPVYAMRS ELIQAVRDNQ
     501  FLVIVGETGS GKTTQITQYL DEEGFSNYGM IGCTQPRRVA AVSVAKRVAE
     551  EVGCKVGHDV GYTIRFEDVT GPDTRIKYMT DGMLQREALL DPEMSKYSVI
     601  MLDEAHERTV ATDVLFALLK KAAIKRPELK VIVTSATLNS AKFSEYFLNC
     651  PIINIPGKTF PVEVLYSQTP QMDYIEAALD CVIDIHINEG PGDILVFLTG
     701  QEEIDSCCEI LYDRVKTLGD SIGELLILPV YSALPSEIQS KIFEPTPKGS
     751  RKVVFATNIA ETSITIDGIY YVVDPGFAKI NIYNARAGIE QLIVSPISQA
     801  QANQRKGRAG RTGPGKCYRL YTESAFYNEM LENTVPEIQR QNLSHTILML
     851  KAMGINDLLK FDFMDPPPKN LMLNALTELY HLQSLDDEGK LTNLGKEMSL
     901  FPMDPTLSRS LLSSVDNQCS DEIVTIISML SVQNVFYRPK DRQLEADSKK
     951  AKFHHPYGDH LTLLNVYTRW QQANYSEQYC KTNFLHFRHL KRARDVKSQI
    1001  SMIFKKIGLK LISCHSDPDL IRKTFVSGFF MNAAKRDSQV GYKTINGGTE
    1051  VGIHPSSSLY GKEYEYVMYH SIVLTSREYM SQVTSIEPQW LLEVAPHFYK
    1101  AGDAESQSRK KAKIIPLHNK FAKDQNSWRL SSIRQSRERA LGIKR*

external links for Prp22p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Prp22p

1)	Vijayraghavan U, et al. (1989) Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev 3(8):1206-16
2)	Schwer B and Gross CH (1998) Prp22, a DExH-box RNA helicase, plays two distinct roles in yeast pre-mRNA splicing. EMBO J 17(7):2086-94
3)	de la Cruz J, et al. (1999) Unwinding RNA in Saccharomyces cerevisiae: DEAD-box proteins and related families. Trends Biochem Sci 24(5):192-8
4)	Wagner JD, et al. (1998) The DEAH-box protein PRP22 is an ATPase that mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes. EMBO J 17(10):2926-37
5)	McPheeters DS and Muhlenkamp P (2003) Spatial organization of protein-RNA interactions in the branch site-3' splice site region during pre-mRNA splicing in yeast. Mol Cell Biol 23(12):4174-86
6)	Umen JG and Guthrie C (1995) The second catalytic step of pre-mRNA splicing. RNA 1(9):869-85
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41