TIR1/YER011W Protein Information

Standard Name	Tir1p
Systematic Name	Yer011wp
Alias	Srp1p
ORF Classification	Verified
Description	Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking (1, 2, 3, 4)
Name Description	TIp1-Related 2

Experimental Data
Molecules/cell	623 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	254
Molecular Weight (Da)	24,906
Isoelectric Point (pI)	4.49

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Tir1p (InterPro)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions	There are 2 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000136FB8 \| P10863 MIPS: YER011W NCBI: 134883 \| 4540 \| 603603 \| 6320848 \| NP_010927.1 GenBank/EMBL/DDBJ: DAA07662.1 \| U18778 \| X12775

Amino Acid Sequence (or in FASTA format)
1 MAYTKIALFA AIAALASAQT QDQINELNVI LNDVKSHLQE YISLASDSSS 51 GFSLSSMPAG VLDIGMALAS ATDDSYTTLY SEVDFAGVSK MLTMVPWYSS 101 RLEPALKSLN GDASSSAAPS SSAAPTSSAA PSSSAAPTSS AASSSSEAKS 151 SSAAPSSSEA KSSSAAPSSS EAKSSSAAPS SSEAKSSSAA PSSTEAKITS 201 AAPSSTGAKT SAISQITDGQ IQATKAVSEQ TENGAAKAFV GMGAGVVAAA 251 AMLL*

Amino Acid Sequence (or in FASTA format)

       1  MAYTKIALFA AIAALASAQT QDQINELNVI LNDVKSHLQE YISLASDSSS
      51  GFSLSSMPAG VLDIGMALAS ATDDSYTTLY SEVDFAGVSK MLTMVPWYSS
     101  RLEPALKSLN GDASSSAAPS SSAAPTSSAA PSSSAAPTSS AASSSSEAKS
     151  SSAAPSSSEA KSSSAAPSSS EAKSSSAAPS SSEAKSSSAA PSSTEAKITS
     201  AAPSSTGAKT SAISQITDGQ IQATKAVSEQ TENGAAKAFV GMGAGVVAAA
     251  AMLL*

external links for Tir1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Tir1p

1)	Kitagaki H, et al. (1997) Identification and analysis of a static culture-specific cell wall protein, Tir1p/Srp1p in Saccharomyces cerevisiae. Eur J Biochem 249(1):343-9
2)	Kowalski LR, et al. (1995) Cold-shock induction of a family of TIP1-related proteins associated with the membrane in Saccharomyces cerevisiae. Mol Microbiol 15(2):341-53
3)	Marguet D, et al. (1988) Yeast gene SRP1 (serine-rich protein). Intragenic repeat structure and identification of a family of SRP1-related DNA sequences. J Mol Biol 202(3):455-70
4)	Cohen BD, et al. (2001) Induction and repression of DAN1 and the family of anaerobic mannoprotein genes in Saccharomyces cerevisiae occurs through a complex array of regulatory sites. Nucleic Acids Res 29(3):799-808
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41