PAC2/YER007W Protein Information

Standard Name	Pac2p 1
Systematic Name	Yer007wp
ORF Classification	Verified
Description	Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl (1, 2, 3, 4)
Name Description	Perish in the Absence of Cin8p 5

Experimental Data
Molecules/cell	2140 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	518
Molecular Weight (Da)	59,257
Isoelectric Point (pI)	8.91

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Pac2p (InterPro)
Physical Interactions	There are 12 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001311CE \| P39937 MIPS: YER007W NCBI: 1330319 \| 603599 \| 6320843 \| 730261 \| NP_010922.1 GenBank/EMBL/DDBJ: DAA07657.1 \| U16814 \| U18778

Amino Acid Sequence (or in FASTA format)
1 MTYEIGDRLK IGGYFCTIKF IGVIKPWPSV KAYGVEWDDH SRGKHSGTID 51 DIHYFDVQIP NSGSFLKESK IKSPGVRRIT FYEALSEKYG GSSNNINDLS 101 IGNKRVEGLG FDELNARNKN YKKLRKIALR DSDVAILFQN QDELNRVIQN 151 CVNVKDLDLS LNLFTNINSL CEFIEPLKNL ESLNISQNKL LSGWDNLKEY 201 DLSHIKTLRL SSCGLSYKHI GKLLKSFRTL KMLDLSYNNL TSAGIQNFEN 251 EIPCTLEELN ISGNNLISFP LFPKNLTLKG LNVSNNQISR APSIAIYSVE 301 SLDITDNKFK ERSLIDDLNK TFPSLKNIHL SGNEFNYNGN YINVEEQATF 351 YEVLARFDRV MVLNGSICDV KTRREAEMFF VSKVMNNELS YDTNLPRWSS 401 LIKSYEIDMS KLSFNNERET RQSLVLKIKI RAGKKPSSDL DYWVLPSFTV 451 RYVKSVICRK LNFDILNVKL FHENSEGMIN EIKYNFRPIS DFNVVNGDII 501 HVSSPVNNKS IQKVNSPS*

Amino Acid Sequence (or in FASTA format)

       1  MTYEIGDRLK IGGYFCTIKF IGVIKPWPSV KAYGVEWDDH SRGKHSGTID
      51  DIHYFDVQIP NSGSFLKESK IKSPGVRRIT FYEALSEKYG GSSNNINDLS
     101  IGNKRVEGLG FDELNARNKN YKKLRKIALR DSDVAILFQN QDELNRVIQN
     151  CVNVKDLDLS LNLFTNINSL CEFIEPLKNL ESLNISQNKL LSGWDNLKEY
     201  DLSHIKTLRL SSCGLSYKHI GKLLKSFRTL KMLDLSYNNL TSAGIQNFEN
     251  EIPCTLEELN ISGNNLISFP LFPKNLTLKG LNVSNNQISR APSIAIYSVE
     301  SLDITDNKFK ERSLIDDLNK TFPSLKNIHL SGNEFNYNGN YINVEEQATF
     351  YEVLARFDRV MVLNGSICDV KTRREAEMFF VSKVMNNELS YDTNLPRWSS
     401  LIKSYEIDMS KLSFNNERET RQSLVLKIKI RAGKKPSSDL DYWVLPSFTV
     451  RYVKSVICRK LNFDILNVKL FHENSEGMIN EIKYNFRPIS DFNVVNGDII
     501  HVSSPVNNKS IQKVNSPS*

external links for Pac2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Pac2p

1)	Hoyt MA, et al. (1997) Saccharomyces cerevisiae PAC2 functions with CIN1, 2 and 4 in a pathway leading to normal microtubule stability. Genetics 146(3):849-57
2)	Fleming JA, et al. (2000) Function of tubulin binding proteins in vivo. Genetics 156(1):69-80
3)	Vega LR, et al. (1998) An alpha-tubulin mutant destabilizes the heterodimer: phenotypic consequences and interactions with tubulin-binding proteins. Mol Biol Cell 9(9):2349-60
4)	Feierbach B, et al. (1999) Alf1p, a CLIP-170 domain-containing protein, is functionally and physically associated with alpha-tubulin. J Cell Biol 144(1):113-24
5)	Geiser JR, et al. (1997) Saccharomyces cerevisiae genes required in the absence of the CIN8-encoded spindle motor act in functionally diverse mitotic pathways. Mol Biol Cell 8(6):1035-50
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41