AFG1/YEL052W Protein Information

Standard Name	Afg1p
Systematic Name	Yel052wp
ORF Classification	Verified
Description	Conserved protein that may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain (1, 2, 3)
Name Description	ATPase Family Gene 1

Experimental Data
Molecules/cell	3570 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	509
Molecular Weight (Da)	58,264
Isoelectric Point (pI)	8.64

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Afg1p (InterPro)
Physical Interactions	There are 4 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000125662 \| P32317 MIPS: YEL052W NCBI: 171040 \| 259060 \| 416589 \| 51013749 \| 603627 \| 6320783 \| NP_010862.1 GenBank/EMBL/DDBJ: DAA07602.1 \| AY693149 \| M94535 \| S47813 \| U18779

Amino Acid Sequence (or in FASTA format)
1 MIALKPNAVR TFRQVQHCSF RICRYQSTKS NKCLTPLQEY DRLVKLGKLR 51 DDTYQRGIIS SLGDLYDSLV KYVPPVVKTP NAVDQVGGWL NGLKSVFSRG 101 KPKNIGAYVD VSKIGNSIPR GVYLYGDVGC GKTMLMDLFY TTIPNHLTKK 151 RIHFHQFMQY VHKRSHEIVR EQNLKELGDA KGKEIDTVPF LAAEIANNSH 201 VLCFDEFQVT DVADAMILRR LMTALLSDDY GVVLFATSNR HPDELYINGV 251 QRQSFIPCIE LIKHRTKVIF LNSPTDYRKI PRPVSSVYYF PSDTSIKYAS 301 KECKTRRETH IKEWYNYFAQ ASHTDDSTDS HTVHKTFYDY PLTIWGREFK 351 VPKCTPPRVA QFTFKQLCGE PLAAGDYLTL AKNFEAFIVT DIPYLSIYVR 401 DEVRRFITFL DAVYDSGGKL ATTGAADFSS LFVEPEQILN DFELRPTTKE 451 PDSVDTGMVD EMVEKHGFSK EIAKKSQMFA LDEERFAFAR ALSRLSQMSS 501 TDWVTKPTY*

Amino Acid Sequence (or in FASTA format)

       1  MIALKPNAVR TFRQVQHCSF RICRYQSTKS NKCLTPLQEY DRLVKLGKLR
      51  DDTYQRGIIS SLGDLYDSLV KYVPPVVKTP NAVDQVGGWL NGLKSVFSRG
     101  KPKNIGAYVD VSKIGNSIPR GVYLYGDVGC GKTMLMDLFY TTIPNHLTKK
     151  RIHFHQFMQY VHKRSHEIVR EQNLKELGDA KGKEIDTVPF LAAEIANNSH
     201  VLCFDEFQVT DVADAMILRR LMTALLSDDY GVVLFATSNR HPDELYINGV
     251  QRQSFIPCIE LIKHRTKVIF LNSPTDYRKI PRPVSSVYYF PSDTSIKYAS
     301  KECKTRRETH IKEWYNYFAQ ASHTDDSTDS HTVHKTFYDY PLTIWGREFK
     351  VPKCTPPRVA QFTFKQLCGE PLAAGDYLTL AKNFEAFIVT DIPYLSIYVR
     401  DEVRRFITFL DAVYDSGGKL ATTGAADFSS LFVEPEQILN DFELRPTTKE
     451  PDSVDTGMVD EMVEKHGFSK EIAKKSQMFA LDEERFAFAR ALSRLSQMSS
     501  TDWVTKPTY*

external links for Afg1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	GeneMANIA
YOGY

References cited on this page View Complete Literature Guide for Afg1p

1)	Lee YJ and Wickner RB (1992) AFG1, a new member of the SEC18-NSF, PAS1, CDC48-VCP, TBP family of ATPases. Yeast 8(9):787-90
2)	Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
3)	Khalimonchuk O, et al. (2007) Evidence for a pro-oxidant intermediate in the assembly of cytochrome oxidase. J Biol Chem 282(24):17442-9
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41