VMA8/YEL051W Protein Information

Standard Name	Vma8p
Systematic Name	Yel051wp
ORF Classification	Verified
Description	Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis (1, 2, 3)
Name Description	Vacuolar Membrane Atpase

Experimental Data
Molecules/cell	8500 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	256
Molecular Weight (Da)	29,194
Isoelectric Point (pI)	5.71

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Vma8p (InterPro)
Physical Interactions	There are 89 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000053074 \| P32610 MIPS: YEL051W NCBI: 418399 \| 603628 \| 6320784 \| NP_010863.1 GenBank/EMBL/DDBJ: DAA07603.1 \| U18779
External Classifications	EC: 3.6.3.14 [H(+)-transporting two-sector ATPase]

Amino Acid Sequence (or in FASTA format)
1 MSGNREQVFP TRMTLGLMKT KLKGANQGYS LLKRKSEALT KRFRDITKRI 51 DDAKQKMGRV MQTAAFSLAE VSYATGENIG YQVQESVSTA RFKVRARQEN 101 VSGVYLSQFE SYIDPEINDF RLTGLGRGGQ QVQRAKEIYS RAVETLVELA 151 SLQTAFIILD EVIKVTNRRV NAIEHVIIPR TENTIAYINS ELDELDREEF 201 YRLKKVQEKK QNETAKLDAE MKLKRDRAEQ DASEVAADEE PQGETLVADQ 251 EDDVIF*

Amino Acid Sequence (or in FASTA format)

       1  MSGNREQVFP TRMTLGLMKT KLKGANQGYS LLKRKSEALT KRFRDITKRI
      51  DDAKQKMGRV MQTAAFSLAE VSYATGENIG YQVQESVSTA RFKVRARQEN
     101  VSGVYLSQFE SYIDPEINDF RLTGLGRGGQ QVQRAKEIYS RAVETLVELA
     151  SLQTAFIILD EVIKVTNRRV NAIEHVIIPR TENTIAYINS ELDELDREEF
     201  YRLKKVQEKK QNETAKLDAE MKLKRDRAEQ DASEVAADEE PQGETLVADQ
     251  EDDVIF*

external links for Vma8p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Vma8p

1)	Graham LA, et al. (1995) VMA8 encodes a 32-kDa V1 subunit of the Saccharomyces cerevisiae vacuolar H(+)-ATPase required for function and assembly of the enzyme complex. J Biol Chem 270(25):15037-44
2)	Xu T and Forgac M (2000) Subunit D (Vma8p) of the yeast vacuolar H+-ATPase plays a role in coupling of proton transport and ATP hydrolysis. J Biol Chem 275(29):22075-81
3)	Forgac M (1999) Structure and properties of the vacuolar (H+)-ATPases. J Biol Chem 274(19):12951-4
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41