MCM3/YEL032W Protein Information Help

Standard Name Mcm3p 1
Systematic Name Yel032wp
ORF Classification Verified
Description Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex (2, 3, 4)
Name Description MiniChromosome Maintenance 5
Experimental Data
Molecules/cell 35100 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 971
Molecular Weight (Da) 107,516
Isoelectric Point (pI) 5.19

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Mcm3p (InterPro)
Physical Interactions There are 112 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000004F9F5 | P24279
MIPS: YEL032W
NCBI: 126822 | 3914 | 603647 | 6320803 | NP_010882.1 | NM_001178847.1
GenBank/EMBL/DDBJ: DAA07621.1 | U18779 | X53540
External Classifications EC: 3.6.4.12 [DNA helicase]
Amino Acid Sequence (or in FASTA format)
 
       1  MEGSTGFDGD ATTFFAPDAV FGDRVRRFQE FLDTFTSYRD SVRSIQVYNS
      51  NNAANYNDDQ DDADERDLLG DDDGDDLEKE KKAASSTSLN ILPHRIIISL
     101  DDLREFDRSF WSGILVEPAY FIPPAEKALT DLADSMDDVP HPNASAVSSR
     151  HPWKLSFKGS FGAHALSPRT LTAQHLNKLV SVEGIVTKTS LVRPKLIRSV
     201  HYAAKTGRFH YRDYTDATTT LTTRIPTPAI YPTEDTEGNK LTTEYGYSTF
     251  IDHQRITVQE MPEMAPAGQL PRSIDVILDD DLVDKTKPGD RVNVVGVFKS
     301  LGAGGMNQSN SNTLIGFKTL ILGNTVYPLH ARSTGVAARQ MLTDFDIRNI
     351  NKLSKKKDIF DILSQSLAPS IYGHDHIKKA ILLMLMGGVE KNLENGSHLR
     401  GDINILMVGD PSTAKSQLLR FVLNTASLAI ATTGRGSSGV GLTAAVTTDR
     451  ETGERRLEAG AMVLADRGVV CIDEFDKMTD VDRVAIHEVM EQQTVTIAKA
     501  GIHTTLNARC SVIAAANPVF GQYDVNRDPH QNIALPDSLL SRFDLLFVVT
     551  DDINEIRDRS ISEHVLRTHR YLPPGYLEGE PVRERLNLSL AVGEDADINP
     601  EEHSNSGAGV ENEGEDDEDH VFEKFNPLLQ AGAKLAKNKG NYNGTEIPKL
     651  VTIPFLRKYV QYAKERVIPQ LTQEAINVIV KNYTDLRNDD NTKKSPITAR
     701  TLETLIRLAT AHAKVRLSKT VNKVDAKVAA NLLRFALLGE DIGNDIDEEE
     751  SEYEEALSKR SPQKSPKKRQ RVRQPASNSG SPIKSTPRRS TASSVNATPS
     801  SARRILRFQD DEQNAGEDDN DIMSPLPADE EAELQRRLQL GLRVSPRRRE
     851  HLHAPEEGSS GPLTEVGTPR LPNVSSAGQD DEQQQSVISF DNVEPGTIST
     901  GRLSLISGII ARLMQTEIFE EESYPVASLF ERINEELPEE EKFSAQEYLA
     951  GLKIMSDRNN LMVADDKVWR V*

external links for Mcm3p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) BioPIXIE MIPS YeastGFP
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Mcm3p
1) Gibson, S.I.  (1989) Analysis of mcm3 in minichromosome maintenance mutant of yeast with a cell division cycle arrest phenotype. Ph.D Thesis
2) Homesley L, et al.  (2000) Mcm10 and the MCM2-7 complex interact to initiate DNA synthesis and to release replication factors from origins. Genes Dev 14(8):913-26
3) Whitbread LA and Dalton S  (1995) Cdc54 belongs to the Cdc46/Mcm3 family of proteins which are essential for initiation of eukaryotic DNA replication. Gene 155(1):113-7
4) Yan H, et al.  (1991) Mcm2 and Mcm3, two proteins important for ARS activity, are related in structure and function. Genes Dev 5(6):944-57
5) Maine GT, et al.  (1984) Mutants of S. cerevisiae defective in the maintenance of minichromosomes. Genetics 106(3):365-85
6) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41