SPF1/YEL031W Protein Information Help

Standard Name Spf1p 1
Systematic Name Yel031wp
Alias Cod1p 2 , Pio1p 3 , Per9p 4
ORF Classification Verified
Description P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 (1, 2, 5, 6)
Name Description Sensitivity to Pichia Farinosa killer toxin 1
Experimental Data
Molecules/cell 1870 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,215
Molecular Weight (Da) 135,267
Isoelectric Point (pI) 6.56

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Spf1p (InterPro)
Transmembrane Domains There are 10 total predicted transmembrane domains (TMHMM)
Physical Interactions There are 13 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052E71 | P39986
MIPS: YEL031W
NCBI: 398364257 | 602398 | 731415 | NP_010883.3
GenBank/EMBL/DDBJ: DAA07622.1 | U18530
External Classifications EC: 3.6.3.- [Hydrolases, Acting on acid anhydrides; catalysing transmembrane movement of substances]
Amino Acid Sequence (or in FASTA format)
 
       1  MTKKSFVSSP IVRDSTLLVP KSLIAKPYVL PFFPLYATFA QLYFQQYDRY
      51  IKGPEWTFVY LGTLVSLNIL VMLMPAWNVK IKAKFNYSTT KNVNEATHIL
     101  IYTTPNNGSD GIVEIQRVTE AGSLQTFFQF QKKRFLWHEN EQVFSSPKFL
     151  VDESPKIGDF QKCKGHSGDL THLKRLYGEN SFDIPIPTFM ELFKEHAVAP
     201  LFVFQVFCVA LWLLDEFWYY SLFNLFMIIS MEAAAVFQRL TALKEFRTMG
     251  IKPYTINVFR NKKWVALQTN ELLPMDLVSI TRTAEESAIP CDLILLDGSA
     301  IVNEAMLSGE STPLLKESIK LRPSEDNLQL DGVDKIAVLH GGTKALQVTP
     351  PEHKSDIPPP PDGGALAIVT KTGFETSQGS LVRVMIYSAE RVSVDNKEAL
     401  MFILFLLIFA VIASWYVWVE GTKMGRIQSK LILDCILIIT SVVPPELPME
     451  LTMAVNSSLA ALAKFYVYCT EPFRIPFAGR IDVCCFDKTG TLTGEDLVFE
     501  GLAGISADSE NIRHLYSAAE APESTILVIG AAHALVKLED GDIVGDPMEK
     551  ATLKAVGWAV ERKNSNYREG TGKLDIIRRF QFSSALKRSA SIASHNDALF
     601  AAVKGAPETI RERLSDIPKN YDEIYKSFTR SGSRVLALAS KSLPKMSQSK
     651  IDDLNRDDVE SELTFNGFLI FHCPLKDDAI ETIKMLNESS HRSIMITGDN
     701  PLTAVHVAKE VGIVFGETLI LDRAGKSDDN QLLFRDVEET VSIPFDPSKD
     751  TFDHSKLFDR YDIAVTGYAL NALEGHSQLR DLLRHTWVYA RVSPSQKEFL
     801  LNTLKDMGYQ TLMCGDGTND VGALKQAHVG IALLNGTEEG LKKLGEQRRL
     851  EGMKMMYIKQ TEFMARWNQP QPPVPEPIAH LFPPGPKNPH YLKALESKGT
     901  VITPEIRKAV EEANSKPVEV IKPNGLSEKK PADLASLLLN SAGDAQGDEA
     951  PALKLGDASC AAPFTSKLAN VSAVTNIIRQ GRCALVNTIQ MYKILALNCL
    1001  ISAYSLSIIY MAGVKFGDGQ ATVSGLLLSV CFLSISRGKP LEKLSKQRPQ
    1051  SGIFNVYIMG SILSQFAVHI ATLVYITTEI YKLEPREPQV DLEKEFAPSL
    1101  LNTGIFIIQL VQQVSTFAVN YQGEPFRENI RSNKGMYYGL LGVTGLALAS
    1151  ATEFLPELNE AMKFVPMTDD FKIKLTLTLL LDFFGSWGVE HFFKFFFMDD
    1201  KPSDISVQQV KIASK*

external links for Spf1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) BioPIXIE MIPS YeastGFP
Candida (CGD) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Spf1p
1) Suzuki C and Shimma YI  (1999) P-type ATPase spf1 mutants show a novel resistance mechanism for the killer toxin SMKT. Mol Microbiol 32(4):813-23
2) Cronin SR, et al.  (2000) Regulation of HMG-CoA reductase degradation requires the P-type ATPase Cod1p/Spf1p. J Cell Biol 148(5):915-24
3) Tipper DJ and Harley CA  (2002) Yeast genes controlling responses to topogenic signals in a model transmembrane protein. Mol Biol Cell 13(4):1158-74
4) Vashist S, et al.  (2002) Two distinctly localized p-type ATPases collaborate to maintain organelle homeostasis required for glycoprotein processing and quality control. Mol Biol Cell 13(11):3955-66
5) Cronin SR, et al.  (2002) Cod1p/Spf1p is a P-type ATPase involved in ER function and Ca2+ homeostasis. J Cell Biol 157(6):1017-28
6) Katrin K, et al.  (2012) Ergosterol content specifies targeting of tail-anchored proteins to mitochondrial outer membranes. Mol Biol Cell 23(20):3927-35
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41