ECM10/YEL030W Protein Information

Standard Name	Ecm10p 1
Systematic Name	Yel030wp
Alias	Ssc3p
ORF Classification	Verified
Description	Heat shock protein of the Hsp70 family; localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; ECM10 has a paralog, SSC1, that arose from the whole genome duplication (2, 3, 4)
Name Description	ExtraCellular Mutant 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	644
Molecular Weight (Da)	70,084
Isoelectric Point (pI)	6.14

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ecm10p (InterPro)
Physical Interactions	There are 31 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013ABEF \| P39987 MIPS: YEL030W NCBI: 602397 \| 6320805 \| 731414 \| NP_010884.1 \| NM_001178845.1 GenBank/EMBL/DDBJ: DAA07623.1 \| U18530

Amino Acid Sequence (or in FASTA format)
1 MLPSWKAFKA HNILRILTRF QSTKIPDAVI GIDLGTTNSA VAIMEGKVPR 51 IIENAEGSRT TPSVVAFTKD GERLVGEPAK RQSVINSENT LFATKRLIGR 101 RFEDAEVQRD INQVPFKIVK HSNGDAWVEA RNRTYSPAQI GGFILNKMKE 151 TAEAYLAKSV KNAVVTVPAY FNDAQRQATK DAGQIIGLNV LRVVNEPTAA 201 ALAYGLDKSE PKVIAVFDLG GGTFDISILD IDNGIFEVKS TNGDTHLGGE 251 DFDIYLLQEI ISHFKKETGI DLSNDRMAVQ RIREAAEKAK IELSSTLSTE 301 INLPFITADA AGPKHIRMPF SRVQLENITA PLIDRTVDPV KKALKDARIT 351 ASDISDVLLV GGMSRMPKVA DTVKKLFGKD ASKAVNPDEA VALGAAIQAA 401 VLSGEVTDVL LLDVTPLSLG IETLGGVFTK LIPRNSTIPN KKSQIFSTAA 451 SGQTSVEVKV FQGERELVKD NKLIGNFTLA GIPPAPKGTP QIEVTFDIDA 501 NGIINVSAKD LASHKDSSIT VAGASGLSDT EIDRMVNEAE RYKNQDRARR 551 NAIETANKAD QLANDTENSI KEFEGKLDKT DSQRLKDQIS SLRELVSRSQ 601 AGDEVNDDDV GTKIDNLRTS SMKLFEQLYK NSDNPETKNG RENK*

Amino Acid Sequence (or in FASTA format)

       1  MLPSWKAFKA HNILRILTRF QSTKIPDAVI GIDLGTTNSA VAIMEGKVPR
      51  IIENAEGSRT TPSVVAFTKD GERLVGEPAK RQSVINSENT LFATKRLIGR
     101  RFEDAEVQRD INQVPFKIVK HSNGDAWVEA RNRTYSPAQI GGFILNKMKE
     151  TAEAYLAKSV KNAVVTVPAY FNDAQRQATK DAGQIIGLNV LRVVNEPTAA
     201  ALAYGLDKSE PKVIAVFDLG GGTFDISILD IDNGIFEVKS TNGDTHLGGE
     251  DFDIYLLQEI ISHFKKETGI DLSNDRMAVQ RIREAAEKAK IELSSTLSTE
     301  INLPFITADA AGPKHIRMPF SRVQLENITA PLIDRTVDPV KKALKDARIT
     351  ASDISDVLLV GGMSRMPKVA DTVKKLFGKD ASKAVNPDEA VALGAAIQAA
     401  VLSGEVTDVL LLDVTPLSLG IETLGGVFTK LIPRNSTIPN KKSQIFSTAA
     451  SGQTSVEVKV FQGERELVKD NKLIGNFTLA GIPPAPKGTP QIEVTFDIDA
     501  NGIINVSAKD LASHKDSSIT VAGASGLSDT EIDRMVNEAE RYKNQDRARR
     551  NAIETANKAD QLANDTENSI KEFEGKLDKT DSQRLKDQIS SLRELVSRSQ
     601  AGDEVNDDDV GTKIDNLRTS SMKLFEQLYK NSDNPETKNG RENK*

external links for Ecm10p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Ecm10p

1)	Lussier M, et al. (1997) Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae. Genetics 147(2):435-50
2)	Baumann F, et al. (2000) Ecm10, a novel hsp70 homolog in the mitochondrial matrix of the yeast Saccharomyces cerevisiae. FEBS Lett 487(2):307-12
3)	Sakasegawa Y, et al. (2003) Ecm10p localizes in yeast mitochondrial nucleoids and its overexpression induces extensive mitochondrial DNA aggregations. Biochem Biophys Res Commun 309(1):217-21
4)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61