RIP1/YEL024W Protein Information

Standard Name	Rip1p 1
Systematic Name	Yel024wp
ORF Classification	Verified
Description	Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly (2, 3, 4, 5)
Name Description	Rieske Iron-sulfur Protein 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	215
Molecular Weight (Da)	23,365
Isoelectric Point (pI)	8.07

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rip1p (InterPro)
Physical Interactions	There are 26 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000137A75 \| P08067 MIPS: YEL024W NCBI: 136715 \| 14277716 \| 145579630 \| 172426 \| 172428 \| 188036284 \| 188036295 \| 188036307 \| 188036318 \| 20151122 \| 20151133 \| 24158775 \| 34811040 \| 398364263 \| 45270572 \| 602391 \| NP_010890.3 GenBank/EMBL/DDBJ: DAA07628.1 \| AY558341 \| M23316 \| M24500 \| U18530
External Classifications	EC: 1.10.2.2 [Ubiquinol--cytochrome-c reductase]

Amino Acid Sequence (or in FASTA format)
1 MLGIRSSVKT CFKPMSLTSK RLISQSLLAS KSTYRTPNFD DVLKENNDAD 51 KGRSYAYFMV GAMGLLSSAG AKSTVETFIS SMTATADVLA MAKVEVNLAA 101 IPLGKNVVVK WQGKPVFIRH RTPHEIQEAN SVDMSALKDP QTDADRVKDP 151 QWLIMLGICT HLGCVPIGEA GDFGGWFCPC HGSHYDISGR IRKGPAPLNL 201 EIPAYEFDGD KVIVG*

Amino Acid Sequence (or in FASTA format)

       1  MLGIRSSVKT CFKPMSLTSK RLISQSLLAS KSTYRTPNFD DVLKENNDAD
      51  KGRSYAYFMV GAMGLLSSAG AKSTVETFIS SMTATADVLA MAKVEVNLAA
     101  IPLGKNVVVK WQGKPVFIRH RTPHEIQEAN SVDMSALKDP QTDADRVKDP
     151  QWLIMLGICT HLGCVPIGEA GDFGGWFCPC HGSHYDISGR IRKGPAPLNL
     201  EIPAYEFDGD KVIVG*

external links for Rip1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Rip1p

1)	Beckmann JD, et al. (1987) Isolation and characterization of the nuclear gene encoding the Rieske iron-sulfur protein (RIP1) from Saccharomyces cerevisiae. J Biol Chem 262(18):8901-9
2)	Ljungdahl PO, et al. (1989) Mutational analysis of the mitochondrial Rieske iron-sulfur protein of Saccharomyces cerevisiae. II. Biochemical characterization of temperature-sensitive RIP1- mutations. J Biol Chem 264(7):3723-31
3)	Graham LA, et al. (1993) Mutational analysis of assembly and function of the iron-sulfur protein of the cytochrome bc1 complex in Saccharomyces cerevisiae. J Bioenerg Biomembr 25(3):245-57
4)	Obungu VH, et al. (1998) The role of charged amino acids in the alpha1-beta4 loop of the iron-sulfur protein of the cytochrome bc1 complex of yeast mitochondria. J Biol Chem 273(19):11917-22
5)	Wagener N, et al. (2011) A pathway of protein translocation in mitochondria mediated by the AAA-ATPase Bcs1. Mol Cell 44(2):191-202