URA3/YEL021W Protein Information

Standard Name	Ura3p 1
Systematic Name	Yel021wp
ORF Classification	Verified
Description	Orotidine-5'-phosphate (OMP) decarboxylase, catalyzes the sixth enzymatic step in the de novo biosynthesis of pyrimidines, converting OMP into uridine monophosphate (UMP); converts 5-FOA into 5-fluorouracil, a toxic compound (1, 2, 3, 4, 5, 6)
Name Description	URAcil requiring 1, 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	267
Molecular Weight (Da)	29,239
Isoelectric Point (pI)	7.37

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ura3p (InterPro)
Physical Interactions	There are 6 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000004C5DD \| P03962 MIPS: YEL021W NCBI: 1326025 \| 172060 \| 172062 \| 1724065 \| 173148 \| 1899165 \| 2190696 \| 241913352 \| 241913353 \| 241913354 \| 241913355 \| 241913356 \| 241913357 \| 241913358 \| 241913359 \| 241913360 \| 241913361 \| 241913362 \| 241913363 \| 241913364 \| 241913365 \| 288562965 \| 288562966 \| 398364267 \| 602388 \| 729303 \| 7546464 \| 7546465 \| 7546466 \| 7546467 \| 7546468 \| 7546469 \| 7546470 \| 7546471 \| NP_010893.3 GenBank/EMBL/DDBJ: DAA07631.1 \| K02206 \| K02207 \| M12926 \| U18530 \| U63018 \| U89671 \| U89927 \| X00191
External Classifications	EC: 4.1.1.23 [Orotidine-5'-phosphate decarboxylase]

Amino Acid Sequence (or in FASTA format)
1 MSKATYKERA ATHPSPVAAK LFNIMHEKQT NLCASLDVRT TKELLELVEA 51 LGPKICLLKT HVDILTDFSM EGTVKPLKAL SAKYNFLLFE DRKFADIGNT 101 VKLQYSAGVY RIAEWADITN AHGVVGPGIV SGLKQAAEEV TKEPRGLLML 151 AELSCKGSLA TGEYTKGTVD IAKSDKDFVI GFIAQRDMGG RDEGYDWLIM 201 TPGVGLDDKG DALGQQYRTV DDVVSTGSDI IIVGRGLFAK GRDAKVEGER 251 YRKAGWEAYL RRCGQQN*

Amino Acid Sequence (or in FASTA format)

       1  MSKATYKERA ATHPSPVAAK LFNIMHEKQT NLCASLDVRT TKELLELVEA
      51  LGPKICLLKT HVDILTDFSM EGTVKPLKAL SAKYNFLLFE DRKFADIGNT
     101  VKLQYSAGVY RIAEWADITN AHGVVGPGIV SGLKQAAEEV TKEPRGLLML
     151  AELSCKGSLA TGEYTKGTVD IAKSDKDFVI GFIAQRDMGG RDEGYDWLIM
     201  TPGVGLDDKG DALGQQYRTV DDVVSTGSDI IIVGRGLFAK GRDAKVEGER
     251  YRKAGWEAYL RRCGQQN*

external links for Ura3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Ura3p

1)	Lacroute F (1968) Regulation of pyrimidine biosynthesis in Saccharomyces cerevisiae. J Bacteriol 95(3):824-32
2)	Alani E and Kleckner N (1987) A new type of fusion analysis applicable to many organisms: protein fusions to the URA3 gene of yeast. Genetics 117(1):5-12
3)	Boeke JD, et al. (1984) A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. Mol Gen Genet 197(2):345-6
4)	Boeke JD, et al. (1987) 5-Fluoroorotic acid as a selective agent in yeast molecular genetics. Methods Enzymol 154():164-75
5)	Jones ME (1992) Orotidylate decarboxylase of yeast and man. Curr Top Cell Regul 33:331-42
6)	Umezu K, et al. (1971) Purification and properties of orotidine-5'-phosphate pyrophosphorylase and orotidine-5'-phosphate decarboxylase from baker's yeast. J Biochem 70(2):249-62
7)	Bach ML, et al. (1979) Evidence for transcriptional regulation of orotidine-5'-phosphate decarboxylase in yeast by hybridization of mRNA to the yeast structural gene cloned in Escherichia coli. Proc Natl Acad Sci U S A 76(1):386-90