VAC8/YEL013W Protein Information

Standard Name	Vac8p
Systematic Name	Yel013wp
Alias	Yeb3p
ORF Classification	Verified
Description	Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions (1, 2, 3, 4)
Name Description	VACuole related

Experimental Data
Molecules/cell	5680 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	578
Molecular Weight (Da)	63,207
Isoelectric Point (pI)	4.86

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Vac8p (InterPro)
Physical Interactions	There are 60 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001380B6 \| P39968 MIPS: YEL013W NCBI: 33337489 \| 398364279 \| 602380 \| 731400 \| NP_010903.3 GenBank/EMBL/DDBJ: DAA07640.1 \| AF005267 \| U18530

Amino Acid Sequence (or in FASTA format)
1 MGSCCSCLKD SSDEASVSPI ADNEREAVTL LLGYLEDKDQ LDFYSGGPLK 51 ALTTLVYSDN LNLQRSAALA FAEITEKYVR QVSREVLEPI LILLQSQDPQ 101 IQVAACAALG NLAVNNENKL LIVEMGGLEP LINQMMGDNV EVQCNAVGCI 151 TNLATRDDNK HKIATSGALI PLTKLAKSKH IRVQRNATGA LLNMTHSEEN 201 RKELVNAGAV PVLVSLLSST DPDVQYYCTT ALSNIAVDEA NRKKLAQTEP 251 RLVSKLVSLM DSPSSRVKCQ ATLALRNLAS DTSYQLEIVR AGGLPHLVKL 301 IQSDSIPLVL ASVACIRNIS IHPLNEGLIV DAGFLKPLVR LLDYKDSEEI 351 QCHAVSTLRN LAASSEKNRK EFFESGAVEK CKELALDSPV SVQSEISACF 401 AILALADVSK LDLLEANILD ALIPMTFSQN QEVSGNAAAA LANLCSRVNN 451 YTKIIEAWDR PNEGIRGFLI RFLKSDYATF EHIALWTILQ LLESHNDKVE 501 DLVKNDDDII NGVRKMADVT FERLQRSGID VKNPGSNNNP SSNDNNSNNN 551 DTGSEHQPVE DASLELYNIT QQILQFLH*

Amino Acid Sequence (or in FASTA format)

       1  MGSCCSCLKD SSDEASVSPI ADNEREAVTL LLGYLEDKDQ LDFYSGGPLK
      51  ALTTLVYSDN LNLQRSAALA FAEITEKYVR QVSREVLEPI LILLQSQDPQ
     101  IQVAACAALG NLAVNNENKL LIVEMGGLEP LINQMMGDNV EVQCNAVGCI
     151  TNLATRDDNK HKIATSGALI PLTKLAKSKH IRVQRNATGA LLNMTHSEEN
     201  RKELVNAGAV PVLVSLLSST DPDVQYYCTT ALSNIAVDEA NRKKLAQTEP
     251  RLVSKLVSLM DSPSSRVKCQ ATLALRNLAS DTSYQLEIVR AGGLPHLVKL
     301  IQSDSIPLVL ASVACIRNIS IHPLNEGLIV DAGFLKPLVR LLDYKDSEEI
     351  QCHAVSTLRN LAASSEKNRK EFFESGAVEK CKELALDSPV SVQSEISACF
     401  AILALADVSK LDLLEANILD ALIPMTFSQN QEVSGNAAAA LANLCSRVNN
     451  YTKIIEAWDR PNEGIRGFLI RFLKSDYATF EHIALWTILQ LLESHNDKVE
     501  DLVKNDDDII NGVRKMADVT FERLQRSGID VKNPGSNNNP SSNDNNSNNN
     551  DTGSEHQPVE DASLELYNIT QQILQFLH*

external links for Vac8p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Vac8p

1)	Pan X, et al. (2000) Nucleus-vacuole junctions in Saccharomyces cerevisiae are formed through the direct interaction of Vac8p with Nvj1p. Mol Biol Cell 11(7):2445-57
2)	Scott SV, et al. (2000) Apg13p and Vac8p are part of a complex of phosphoproteins that are required for cytoplasm to vacuole targeting. J Biol Chem 275(33):25840-9
3)	Wang YX, et al. (1998) Vac8p, a vacuolar protein with armadillo repeats, functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole. J Cell Biol 140(5):1063-74
4)	Subramanian K, et al. (2006) Palmitoylation determines the function of Vac8 at the yeast vacuole. J Cell Sci 119(Pt 12):2477-85
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41