UBC8/YEL012W Protein Information

Standard Name	Ubc8p 1
Systematic Name	Yel012wp
Alias	Gid3p 2, 3
ORF Classification	Verified
Description	Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro (1, 4, 5)
Name Description	UBiquitin-Conjugating 1

Experimental Data
Molecules/cell	1590 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	218
Molecular Weight (Da)	24,633
Isoelectric Point (pI)	3.91

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ubc8p (InterPro)
Physical Interactions	There are 14 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013796B \| P28263 MIPS: YEL012W NCBI: 483453 \| 549146 \| 602379 \| 6579192 \| NP_010904.2 \| NM_001178827.1 GenBank/EMBL/DDBJ: DAA07641.1 \| M65083 \| U18530 \| Z29329
External Classifications	EC: 6.3.2.19 [Ubiquitin--protein ligase]

Amino Acid Sequence (or in FASTA format)
1 MSSSKRRIET DVMKLLMSDH QVDLINDSMQ EFHVKFLGPK DTPYENGVWR 51 LHVELPDNYP YKSPSIGFVN KIFHPNIDIA SGSICLDVIN STWSPLYDLI 101 NIVEWMIPGL LKEPNGSDPL NNEAATLQLR DKKLYEEKIK EYIDKYATKE 151 KYQQMFGGDN DSDDSDSGGD LQEEDSDSDE DMDGTGVSSG DDSVDELSED 201 LSDIDVSDDD DYDEVANQ*

Amino Acid Sequence (or in FASTA format)

       1  MSSSKRRIET DVMKLLMSDH QVDLINDSMQ EFHVKFLGPK DTPYENGVWR
      51  LHVELPDNYP YKSPSIGFVN KIFHPNIDIA SGSICLDVIN STWSPLYDLI
     101  NIVEWMIPGL LKEPNGSDPL NNEAATLQLR DKKLYEEKIK EYIDKYATKE
     151  KYQQMFGGDN DSDDSDSGGD LQEEDSDSDE DMDGTGVSSG DDSVDELSED
     201  LSDIDVSDDD DYDEVANQ*

external links for Ubc8p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Ubc8p

1)	Qin S, et al. (1991) Cloning and characterization of a Saccharomyces cerevisiae gene encoding a new member of the ubiquitin-conjugating protein family. J Biol Chem 266(23):15549-54
2)	Schule T, et al. (2000) Ubc8p functions in catabolite degradation of fructose-1, 6-bisphosphatase in yeast. EMBO J 19(10):2161-7
3)	Hammerle M, et al. (1998) Proteins of newly isolated mutants and the amino-terminal proline are essential for ubiquitin-proteasome-catalyzed catabolite degradation of fructose-1,6-bisphosphatase of Saccharomyces cerevisiae. J Biol Chem 273(39):25000-5
4)	Kaiser P, et al. (1994) A human ubiquitin-conjugating enzyme homologous to yeast UBC8. J Biol Chem 269(12):8797-802
5)	Regelmann J, et al. (2003) Catabolite degradation of fructose-1,6-bisphosphatase in the yeast Saccharomyces cerevisiae: a genome-wide screen identifies eight novel GID genes and indicates the existence of two degradation pathways. Mol Biol Cell 14(4):1652-63
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41