TUP1/YCR084C Protein Information

Standard Name	Tup1p
Systematic Name	Ycr084cp
Alias	Aar1p , Aer2p , Amm1p , Crt4p 1 , Cyc9p 2, 3 , Flk1p 4 , Rox4p , Sfl2p , Umr7p 3
ORF Classification	Verified
Description	General repressor of transcription, forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes (10, 5, 6, 7, 8, 9)
Name Description	deoxyThymidine monophosphate UPtake

Experimental Data
Molecules/cell	5840 11

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	713
Molecular Weight (Da)	78,307
Isoelectric Point (pI)	5.47

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Tup1p (InterPro)
Physical Interactions	There are 118 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000005313F \| P16649 MIPS: YCR084C NCBI: 136482 \| 171038 \| 173067 \| 1907221 \| 390980918 \| 390980919 \| 390980920 \| 390980921 \| 390980922 \| 390980923 \| 4460 \| 6319926 \| 9955107 \| 9955108 \| 9955109 \| NP_010007.1 \| NM_001178790.1 GenBank/EMBL/DDBJ: DAA07554.1 \| M31733 \| M35861 \| X16365 \| X59720

Amino Acid Sequence (or in FASTA format)
1 MTASVSNTQN KLNELLDAIR QEFLQVSQEA NTYRLQNQKD YDFKMNQQLA 51 EMQQIRNTVY ELELTHRKMK DAYEEEIKHL KLGLEQRDHQ IASLTVQQQR 101 QQQQQQQVQQ HLQQQQQQLA AASASVPVAQ QPPATTSATA TPAANTTTGS 151 PSAFPVQASR PNLVGSQLPT TTLPVVSSNA QQQLPQQQLQ QQQLQQQQPP 201 PQVSVAPLSN TAINGSPTSK ETTTLPSVKA PESTLKETEP ENNNTSKIND 251 TGSATTATTT TATETEIKPK EEDATPASLH QDHYLVPYNQ RANHSKPIPP 301 FLLDLDSQSV PDALKKQTND YYILYNPALP REIDVELHKS LDHTSVVCCV 351 KFSNDGEYLA TGCNKTTQVY RVSDGSLVAR LSDDSAANNH RNSITENNTT 401 TSTDNNTMTT TTTTTITTTA MTSAAELAKD VENLNTSSSP SSDLYIRSVC 451 FSPDGKFLAT GAEDRLIRIW DIENRKIVMI LQGHEQDIYS LDYFPSGDKL 501 VSGSGDRTVR IWDLRTGQCS LTLSIEDGVT TVAVSPGDGK YIAAGSLDRA 551 VRVWDSETGF LVERLDSENE SGTGHKDSVY SVVFTRDGQS VVSGSLDRSV 601 KLWNLQNANN KSDSKTPNSG TCEVTYIGHK DFVLSVATTQ NDEYILSGSK 651 DRGVLFWDKK SGNPLLMLQG HRNSVISVAV ANGSPLGPEY NVFATGSGDC 701 KARIWKYKKI APN*

Amino Acid Sequence (or in FASTA format)

       1  MTASVSNTQN KLNELLDAIR QEFLQVSQEA NTYRLQNQKD YDFKMNQQLA
      51  EMQQIRNTVY ELELTHRKMK DAYEEEIKHL KLGLEQRDHQ IASLTVQQQR
     101  QQQQQQQVQQ HLQQQQQQLA AASASVPVAQ QPPATTSATA TPAANTTTGS
     151  PSAFPVQASR PNLVGSQLPT TTLPVVSSNA QQQLPQQQLQ QQQLQQQQPP
     201  PQVSVAPLSN TAINGSPTSK ETTTLPSVKA PESTLKETEP ENNNTSKIND
     251  TGSATTATTT TATETEIKPK EEDATPASLH QDHYLVPYNQ RANHSKPIPP
     301  FLLDLDSQSV PDALKKQTND YYILYNPALP REIDVELHKS LDHTSVVCCV
     351  KFSNDGEYLA TGCNKTTQVY RVSDGSLVAR LSDDSAANNH RNSITENNTT
     401  TSTDNNTMTT TTTTTITTTA MTSAAELAKD VENLNTSSSP SSDLYIRSVC
     451  FSPDGKFLAT GAEDRLIRIW DIENRKIVMI LQGHEQDIYS LDYFPSGDKL
     501  VSGSGDRTVR IWDLRTGQCS LTLSIEDGVT TVAVSPGDGK YIAAGSLDRA
     551  VRVWDSETGF LVERLDSENE SGTGHKDSVY SVVFTRDGQS VVSGSLDRSV
     601  KLWNLQNANN KSDSKTPNSG TCEVTYIGHK DFVLSVATTQ NDEYILSGSK
     651  DRGVLFWDKK SGNPLLMLQG HRNSVISVAV ANGSPLGPEY NVFATGSGDC
     701  KARIWKYKKI APN*

external links for Tup1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Tup1p

1)	Zhou Z and Elledge SJ (1992) Isolation of crt mutants constitutive for transcription of the DNA damage inducible gene RNR3 in Saccharomyces cerevisiae. Genetics 131(4):851-66
2)	Rothstein RJ and Sherman F (1980) Genes affecting the expression of cytochrome c in yeast: genetic mapping and genetic interactions. Genetics 94(4):871-89
3)	Lemontt JF, et al. (1980) Pleiotropic Mutations at the TUP1 Locus That Affect the Expression of Mating-Type-Dependent Functions in SACCHAROMYCES CEREVISIAE. Genetics 94(4):899-920
4)	Stark HC, et al. (1980) Pleiotropic properties of a yeast mutant insensitive to catabolite repression. Genetics 94(4):921-8
5)	Edmondson DG, et al. (1996) Repression domain of the yeast global repressor Tup1 interacts directly with histones H3 and H4. Genes Dev 10(10):1247-59
6)	Cooper JP, et al. (1994) The global transcriptional regulators, SSN6 and TUP1, play distinct roles in the establishment of a repressive chromatin structure. Genes Dev 8(12):1400-10
7)	Williams FE, et al. (1991) The CYC8 and TUP1 proteins involved in glucose repression in Saccharomyces cerevisiae are associated in a protein complex. Mol Cell Biol 11(6):3307-16
8)	Zhang L and Guarente L (1994) Evidence that TUP1/SSN6 has a positive effect on the activity of the yeast activator HAP1. Genetics 136(3):813-7
9)	Keleher CA, et al. (1992) Ssn6-Tup1 is a general repressor of transcription in yeast. Cell 68(4):709-19
10)	Conlan RS, et al. (1999) The Tup1-Cyc8 protein complex can shift from a transcriptional co-repressor to a transcriptional co-activator. J Biol Chem 274(1):205-10
11)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41