SRB8/YCR081W Protein Information

Standard Name	Srb8p 1
Systematic Name	Ycr081wp
Alias	Gig1p 2 , Nut6p 3 , Ssn5p , Ycr080wp , Med12p 4
ORF Classification	Verified
Description	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression (1, 2, 5)
Name Description	Suppressor of RNA polymerase B 1

Experimental Data
Molecules/cell	1730 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,427
Molecular Weight (Da)	166,859
Isoelectric Point (pI)	6.47

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Srb8p (InterPro)
Physical Interactions	There are 54 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000053160 \| P25648 MIPS: YCR081W NCBI: 1907218 \| 6094350 \| 6319923 \| NP_010004.1 \| NM_001178787.1 GenBank/EMBL/DDBJ: DAA07551.1 \| X59720

Amino Acid Sequence (or in FASTA format)
1 MNNGSGRYLL TPPDDLHPYV PSSKPQEQVY PDFKPWEHTA AEDQILANFV 51 AKGFYHTPMV NFESISARSS VHESLVTQSN ILSQQFDKII KIREDHINKI 101 PSNSTTTLHG PGFQLPNRIT LTDHRKETWL HELSSSHTSL VKIGKFIPHG 151 LKRRQVIEQC YLKFIPLKRA IWLIKCCYFI EWKSNHKKKR SNAAGADDAI 201 SMHLLKDWTD TFVYILEKLI FDMTNHYNDS QQLRTWKRQI SYFLKLLGNC 251 YSLRLINKEI FHHWLVEFIN KMENFEFLPL SLHILMIFWN DICQIDTNAP 301 VAATITSSQK EPFFLVTKIT DMLLHKYYIV SSSKSMINDE NYIINDIKKN 351 NKIKLNILKI LSSLILKIFQ EQSLEVFIFP TSNWEIYKPL LFEIVSNADT 401 NQNSDMKKKL ELISYRNESL KNNSSIRNVI MSASNANDFQ LTIVTCKQFP 451 KLSCIQLNCI DTQFTKLLDD NPTEFDWPTY VDQNPLTMHK IIQLILWSIH 501 PSRQFDHYES NQLVAKLLLL RINSTDEDLH EFQIEDAIWS LVFQLAKNFS 551 AQKRVVSYMM PSLYRLLNIL ITYGIIKVPT YIRKLISSGL LYLQDSNDKF 601 VHVQLLINLK ISPLMKSQYN MVLRNVMEYD VKFYEIFNFD QLVEITEQIK 651 MRILSNDITN LQLSKTPLSI KIMVAEWYLS HLCSGILSSV NRTVLLKIFK 701 IFCIDLEVFH HFFKWIEFIV YHQLLSDIES LEALMDILLC YQKLFSQFIN 751 DHILFTKTFI FIYKKVLKEK DVPAYNVTSF MPFWKFFMKN FPFVLKVDND 801 LRIELQSVYN DEKLKTEKLK NDKSEVLKVY SMINNSNQAV GQTWNFPEVF 851 QVNIRFLLHN SEIIDTNTSK QFQKARNNVM LLIATNLKEY NKFMSIFLKR 901 KDFTNKNLIQ LISLKLLTFE VTQNVLGLEY IIRLLPINLE NNDGSYGLFL 951 KYHKEQFIKS NFEKILLTCY ELEKKYHGNE CEINYYEILL KILITYGSSP 1001 KLLATSTKII MLLLNDSVEN SSNILEDILY YSTCPSETDL NDIPLGSGQP 1051 DNDTVVTNDD KSDDDDHTVD EIDHVEYYVM MDFANLWVFQ AFTCFCIKKI 1101 MENNEPAMAM EDLKNFIFQI IEITNSNDLC SQIFDQLKDM QTIEMITQIV 1151 EKDFCTSCLQ NNNQKIDDNY IVVVIEIITS LSMRFQRETS GMIVISMENY 1201 HLLIKIIRQL SELNEGNLSK REIQIDAVLK IFSFHQDSIF QRIIADLSAD 1251 KPTSPFIDSI CKLFDKISFN LRLKLFLYEI LSSLKSFAIY SSTIDAPAFH 1301 TSGKVELPKK LLNLPPFQVS SFVKETKLHS GDYGEEEDAD QEESFSLNLG 1351 IGIVEIAHEN EQKWLIYDKK DHKYVCTFSM EPYHFISNYN TKYTDDMATG 1401 SNDTTAFNDS CVNLSLFDAR FERKNPH*

Amino Acid Sequence (or in FASTA format)

       1  MNNGSGRYLL TPPDDLHPYV PSSKPQEQVY PDFKPWEHTA AEDQILANFV
      51  AKGFYHTPMV NFESISARSS VHESLVTQSN ILSQQFDKII KIREDHINKI
     101  PSNSTTTLHG PGFQLPNRIT LTDHRKETWL HELSSSHTSL VKIGKFIPHG
     151  LKRRQVIEQC YLKFIPLKRA IWLIKCCYFI EWKSNHKKKR SNAAGADDAI
     201  SMHLLKDWTD TFVYILEKLI FDMTNHYNDS QQLRTWKRQI SYFLKLLGNC
     251  YSLRLINKEI FHHWLVEFIN KMENFEFLPL SLHILMIFWN DICQIDTNAP
     301  VAATITSSQK EPFFLVTKIT DMLLHKYYIV SSSKSMINDE NYIINDIKKN
     351  NKIKLNILKI LSSLILKIFQ EQSLEVFIFP TSNWEIYKPL LFEIVSNADT
     401  NQNSDMKKKL ELISYRNESL KNNSSIRNVI MSASNANDFQ LTIVTCKQFP
     451  KLSCIQLNCI DTQFTKLLDD NPTEFDWPTY VDQNPLTMHK IIQLILWSIH
     501  PSRQFDHYES NQLVAKLLLL RINSTDEDLH EFQIEDAIWS LVFQLAKNFS
     551  AQKRVVSYMM PSLYRLLNIL ITYGIIKVPT YIRKLISSGL LYLQDSNDKF
     601  VHVQLLINLK ISPLMKSQYN MVLRNVMEYD VKFYEIFNFD QLVEITEQIK
     651  MRILSNDITN LQLSKTPLSI KIMVAEWYLS HLCSGILSSV NRTVLLKIFK
     701  IFCIDLEVFH HFFKWIEFIV YHQLLSDIES LEALMDILLC YQKLFSQFIN
     751  DHILFTKTFI FIYKKVLKEK DVPAYNVTSF MPFWKFFMKN FPFVLKVDND
     801  LRIELQSVYN DEKLKTEKLK NDKSEVLKVY SMINNSNQAV GQTWNFPEVF
     851  QVNIRFLLHN SEIIDTNTSK QFQKARNNVM LLIATNLKEY NKFMSIFLKR
     901  KDFTNKNLIQ LISLKLLTFE VTQNVLGLEY IIRLLPINLE NNDGSYGLFL
     951  KYHKEQFIKS NFEKILLTCY ELEKKYHGNE CEINYYEILL KILITYGSSP
    1001  KLLATSTKII MLLLNDSVEN SSNILEDILY YSTCPSETDL NDIPLGSGQP
    1051  DNDTVVTNDD KSDDDDHTVD EIDHVEYYVM MDFANLWVFQ AFTCFCIKKI
    1101  MENNEPAMAM EDLKNFIFQI IEITNSNDLC SQIFDQLKDM QTIEMITQIV
    1151  EKDFCTSCLQ NNNQKIDDNY IVVVIEIITS LSMRFQRETS GMIVISMENY
    1201  HLLIKIIRQL SELNEGNLSK REIQIDAVLK IFSFHQDSIF QRIIADLSAD
    1251  KPTSPFIDSI CKLFDKISFN LRLKLFLYEI LSSLKSFAIY SSTIDAPAFH
    1301  TSGKVELPKK LLNLPPFQVS SFVKETKLHS GDYGEEEDAD QEESFSLNLG
    1351  IGIVEIAHEN EQKWLIYDKK DHKYVCTFSM EPYHFISNYN TKYTDDMATG
    1401  SNDTTAFNDS CVNLSLFDAR FERKNPH*

external links for Srb8p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
YGOB	CYC2008 (complexes)	Pfam domains
YOGY	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Srb8p

1)	Hengartner CJ, et al. (1995) Association of an activator with an RNA polymerase II holoenzyme. Genes Dev 9(8):897-910
2)	Balciunas D and Ronne H (1995) Three subunits of the RNA polymerase II mediator complex are involved in glucose repression. Nucleic Acids Res 23(21):4421-5
3)	Tabtiang RK and Herskowitz I (1998) Nuclear proteins Nut1p and Nut2p cooperate to negatively regulate a Swi4p-dependent lacZ reporter gene in Saccharomyces cerevisiae. Mol Cell Biol 18(8):4707-18
4)	Bourbon HM, et al. (2004) A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. Mol Cell 14(5):553-7
5)	Kornberg RD (2005) Mediator and the mechanism of transcriptional activation. Trends Biochem Sci 30(5):235-9
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41