YIH1/YCR059C Protein Information

Standard Name	Yih1p 1
Systematic Name	Ycr059cp
ORF Classification	Verified
Description	Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT (1, 2, 3, 4, 5)
Name Description	Yeast Impact Homolog 1

Experimental Data
Molecules/cell	3030 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	258
Molecular Weight (Da)	29,017
Isoelectric Point (pI)	4.36

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Yih1p (InterPro)
Physical Interactions	There are 23 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013A723 \| P25637 MIPS: YCR059C NCBI: 140519 \| 1907199 \| 51013677 \| 6319904 \| NP_009985.1 GenBank/EMBL/DDBJ: DAA07533.1 \| AY693113 \| X59720

Amino Acid Sequence (or in FASTA format)
1 MDDDHEQLVE ELEAVEAIYP DLLSKKQEDG SIIVVKVPQH EYMTLQISFP 51 THYPSEEAPN VIEVGVCTSL AKRDLYDTKY LQHLFQEVMD SVFHRGSVCL 101 FDFLTELDGV LYVEPEEETE PVQQSDIPTD PFEGWTASDP ITDRGSTFMA 151 FAAHVTSEEQ AFAMLDLLKT DSKMRKANHV MSAWRIKQDG SAATYQDSDD 201 DGETAAGSRM LHLITIMDVW NVIVVVARWF GGAHIGPDRF KHINSTAREA 251 VVRAGFDS*

Amino Acid Sequence (or in FASTA format)

       1  MDDDHEQLVE ELEAVEAIYP DLLSKKQEDG SIIVVKVPQH EYMTLQISFP
      51  THYPSEEAPN VIEVGVCTSL AKRDLYDTKY LQHLFQEVMD SVFHRGSVCL
     101  FDFLTELDGV LYVEPEEETE PVQQSDIPTD PFEGWTASDP ITDRGSTFMA
     151  FAAHVTSEEQ AFAMLDLLKT DSKMRKANHV MSAWRIKQDG SAATYQDSDD
     201  DGETAAGSRM LHLITIMDVW NVIVVVARWF GGAHIGPDRF KHINSTAREA
     251  VVRAGFDS*

external links for Yih1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Yih1p

1)	Kubota H, et al. (2000) GI domain-mediated association of the eukaryotic initiation factor 2alpha kinase GCN2 with its activator GCN1 is required for general amino acid control in budding yeast. J Biol Chem 275(27):20243-6
2)	Sattlegger E, et al. (2004) YIH1 is an actin-binding protein that inhibits protein kinase GCN2 and impairs general amino acid control when overexpressed. J Biol Chem 279(29):29952-62
3)	Pereira CM, et al. (2005) IMPACT, a protein preferentially expressed in the mouse brain, binds GCN1 and inhibits GCN2 activation. J Biol Chem 280(31):28316-23
4)	Sattlegger E, et al. (2011) Gcn1 and Actin Binding to Yih1: IMPLICATIONS FOR ACTIVATION OF THE eIF2 KINASE GCN2. J Biol Chem 286(12):10341-55
5)	Waller T, et al. (2012) Evidence that Yih1 resides in a complex with ribosomes. FEBS J 279(10):1761-76
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41