HMLALPHA2/YCL067C Protein Information

Standard Name	Hmlalpha2p
Systematic Name	Ycl067cp
Alias	Alpha2p
ORF Classification	Verified
Description	Silenced copy of ALPHA2 at HML; homeobox-domain protein that associates with Mcm1p in haploid cells to repress a-specific gene expression and interacts with a1p in diploid cells to repress haploid-specific gene expression (1, 2, 3, 4, 5, 6, 7, 8, 9)
Name Description	Hidden Mat Left ALPHA

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	210
Molecular Weight (Da)	24,282
Isoelectric Point (pI)	10.24

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Hmlalpha2p (InterPro)
Physical Interactions	There are 5 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012EBF8 \| P0CY09 MIPS: YCL067C NCBI: 171690 \| 338810318 \| 3897 \| 5292 \| 578189 \| 6319785 \| 63536787 \| NP_009866.1 \| NP_009868.3 GenBank/EMBL/DDBJ: DAA07421.1 \| L00059 \| V01314 \| V01315 \| X59720

Amino Acid Sequence (or in FASTA format)
1 MNKIPIKDLL NPQITDEFKS SILDINKKLF SICCNLPKLP ESVTTEEEVE 51 LRDILGFLSR ANKNRKISDE EKKLLQTTSQ LTTTITVLLK EMRSIENDRS 101 NYQLTQKNKS ADGLVFNVVT QDMINKSTKP YRGHRFTKEN VRILESWFAK 151 NIENPYLDTK GLENLMKNTS LSRIQIKNWV SNRRRKEKTI TIAPELADLL 201 SGEPLAKKKE *

Amino Acid Sequence (or in FASTA format)

       1  MNKIPIKDLL NPQITDEFKS SILDINKKLF SICCNLPKLP ESVTTEEEVE
      51  LRDILGFLSR ANKNRKISDE EKKLLQTTSQ LTTTITVLLK EMRSIENDRS
     101  NYQLTQKNKS ADGLVFNVVT QDMINKSTKP YRGHRFTKEN VRILESWFAK
     151  NIENPYLDTK GLENLMKNTS LSRIQIKNWV SNRRRKEKTI TIAPELADLL
     201  SGEPLAKKKE *

external links for Hmlalpha2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	GeneMANIA

References cited on this page View Complete Literature Guide for Hmlalpha2p

1)	Goutte C and Johnson AD (1988) a1 protein alters the DNA binding specificity of alpha 2 repressor. Cell 52(6):875-82
2)	Murphy MR, et al. (1993) DNA-protein interactions at the S.cerevisiae alpha 2 operator in vivo. Nucleic Acids Res 21(14):3295-300
3)	Vershon AK and Johnson AD (1993) A short, disordered protein region mediates interactions between the homeodomain of the yeast alpha 2 protein and the MCM1 protein. Cell 72(1):105-12
4)	Goutte C and Johnson AD (1994) Recognition of a DNA operator by a dimer composed of two different homeodomain proteins. EMBO J 13(6):1434-42
5)	Zhong H, et al. (1999) Identification of target sites of the alpha2-Mcm1 repressor complex in the yeast genome. Genome Res 9(11):1040-7
6)	Goutte C and Johnson AD (1993) Yeast a1 and alpha 2 homeodomain proteins form a DNA-binding activity with properties distinct from those of either protein. J Mol Biol 233(3):359-71
7)	Smith DL and Johnson AD (1992) A molecular mechanism for combinatorial control in yeast: MCM1 protein sets the spacing and orientation of the homeodomains of an alpha 2 dimer. Cell 68(1):133-42
8)	Herskowitz I (1989) A regulatory hierarchy for cell specialization in yeast. Nature 342(6251):749-57
9)	Herskowitz I, et al. (1992) "Mating-type determination and mating-type interconversion in Saccharomyces cerevisiae." Pp. 583-656 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press