SPB1/YCL054W Protein Information Help

Standard Name Spb1p
Systematic Name Ycl054wp
ORF Classification Verified
Description AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants (1, 2, 3)
Name Description Suppressor of PaB1 mutant 4
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 841
Molecular Weight (Da) 96,484
Isoelectric Point (pI) 8.2

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Spb1p (InterPro)
Physical Interactions There are 48 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000053220 | P25582
MIPS: YCL054W
NCBI: 1907120 | 6226708 | 6319796 | NP_009877.1
GenBank/EMBL/DDBJ: DAA07432.1 | X59720
External Classifications EC: 2.1.1.- [Transferases transferring one-carbon groups methyltransferases]
Amino Acid Sequence (or in FASTA format)
 
       1  MGKTQKKNSK GRLDRYYYLA KEKGYRARSS FKIIQINEKY GHFLEKSKVV
      51  IDLCAAPGSW CQVASKLCPV NSLIIGVDIV PMKPMPNVIT FQSDITTEDC
     101  RSKLRGYMKT WKADTVLHDG APNVGLGWVQ DAFTQSQLTL QALKLAVENL
     151  VVNGTFVTKI FRSKDYNKLI WVFQQLFEKV EATKPPASRN VSAEIFVVCK
     201  GFKAPKRLDP RLLDPKEVFE ELPDGQQNME SKIYNPEKKV RKRQGYEEGD
     251  NLLYHETSIL DFVRTEDPIS MLGEMNKFTI DENDHEWKIL KKLKQTTDEF
     301  RSCIEDLKVL GKKDFKMILR WRKIAREILG IEVKDDAKTE IEVVPLTEEE
     351  QIEKDLQGLQ EKQRLNVKRE RRRKNEMKQK ELQRMQMNMI TPTDIGIEAA
     401  SLGKESLFNL KTAEKTGILN DLAKGKKRMI FTDDELAKDN DIYIDENIMI
     451  KDKDSAADAD DLESELNAMY SDYKTRRSER DAKFRAKQAR GGDNEEEWTG
     501  FNEGSLEKKE EEGKDYIEDN DDEGVEGDSD DDEAITNLIS KLKGQEGDHK
     551  LSSKARMIFN DPIFNNVEPD LPVNTVNDGI MSSESVGDIS KLNKKRKHEE
     601  MHQKQDEADS SDESSSDDSD FEIVANDNAS EEFDSDYDSE EEKNQTKKEK
     651  HSRDIDIATV EAMTLAHQLA LGQKNKHDLV DEGFNRYTFR DTENLPDWFL
     701  EDEKEHSKIN KPITKEAAMA IKEKIKAMNA RPIKKVAEAK ARKRMRAVAR
     751  LEKIKKKAGL INDDSDKTEK DKAEEISRLM RKVTKKPKTK PKVTLVVASG
     801  RNKGLAGRPK GVKGKYKMVD GVMKNEQRAL RRIAKKHHKK K*

external links for Spb1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Spb1p
1) Kressler D, et al.  (1999) Spb1p is a putative methyltransferase required for 60S ribosomal subunit biogenesis in Saccharomyces cerevisiae. Nucleic Acids Res 27(23):4598-608
2) Bonnerot C, et al.  (2003) Functional redundancy of Spb1p and a snR52-dependent mechanism for the 2'-O-ribose methylation of a conserved rRNA position in yeast. Mol Cell 12(5):1309-15
3) Lapeyre B and Purushothaman SK  (2004) Spb1p-directed formation of Gm2922 in the ribosome catalytic center occurs at a late processing stage. Mol Cell 16(4):663-9
4) Sachs AB and Davis RW  (1989) The poly(A) binding protein is required for poly(A) shortening and 60S ribosomal subunit-dependent translation initiation. Cell 58(5):857-67