GLK1/YCL040W Protein Information

Standard Name	Glk1p
Systematic Name	Ycl040wp
Alias	Hor3p 1
ORF Classification	Verified
Description	Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication (2, 3, 4)
Name Description	GLucoKinase

Experimental Data
Molecules/cell	21100 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	500
Molecular Weight (Da)	55,377
Isoelectric Point (pI)	6.14

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Glk1p (InterPro)
Physical Interactions	There are 19 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012CFB3 \| P17709 MIPS: YCL040W NCBI: 123899 \| 5323 \| 577521 \| 6319809 \| NP_009890.1 GenBank/EMBL/DDBJ: DAA07444.1 \| M24077 \| X59720
External Classifications	EC: 2.7.1.1 [Hexokinase] EC: 2.7.1.2 [Glucokinase]

Amino Acid Sequence (or in FASTA format)
1 MSFDDLHKAT ERAVIQAVDQ ICDDFEVTPE KLDELTAYFI EQMEKGLAPP 51 KEGHTLASDK GLPMIPAFVT GSPNGTERGV LLAADLGGTN FRICSVNLHG 101 DHTFSMEQMK SKIPDDLLDD ENVTSDDLFG FLARRTLAFM KKYHPDELAK 151 GKDAKPMKLG FTFSYPVDQT SLNSGTLIRW TKGFRIADTV GKDVVQLYQE 201 QLSAQGMPMI KVVALTNDTV GTYLSHCYTS DNTDSMTSGE ISEPVIGCIF 251 GTGTNGCYME EINKITKLPQ ELRDKLIKEG KTHMIINVEW GSFDNELKHL 301 PTTKYDVVID QKLSTNPGFH LFEKRVSGMF LGEVLRNILV DLHSQGLLLQ 351 QYRSKEQLPR HLTTPFQLSS EVLSHIEIDD STGLRETELS LLQSLRLPTT 401 PTERVQIQKL VRAISRRSAY LAAVPLAAIL IKTNALNKRY HGEVEIGCDG 451 SVVEYYPGFR SMLRHALALS PLGAEGERKV HLKIAKDGSG VGAALCALVA 501 *

Amino Acid Sequence (or in FASTA format)

       1  MSFDDLHKAT ERAVIQAVDQ ICDDFEVTPE KLDELTAYFI EQMEKGLAPP
      51  KEGHTLASDK GLPMIPAFVT GSPNGTERGV LLAADLGGTN FRICSVNLHG
     101  DHTFSMEQMK SKIPDDLLDD ENVTSDDLFG FLARRTLAFM KKYHPDELAK
     151  GKDAKPMKLG FTFSYPVDQT SLNSGTLIRW TKGFRIADTV GKDVVQLYQE
     201  QLSAQGMPMI KVVALTNDTV GTYLSHCYTS DNTDSMTSGE ISEPVIGCIF
     251  GTGTNGCYME EINKITKLPQ ELRDKLIKEG KTHMIINVEW GSFDNELKHL
     301  PTTKYDVVID QKLSTNPGFH LFEKRVSGMF LGEVLRNILV DLHSQGLLLQ
     351  QYRSKEQLPR HLTTPFQLSS EVLSHIEIDD STGLRETELS LLQSLRLPTT
     401  PTERVQIQKL VRAISRRSAY LAAVPLAAIL IKTNALNKRY HGEVEIGCDG
     451  SVVEYYPGFR SMLRHALALS PLGAEGERKV HLKIAKDGSG VGAALCALVA
     501  *

external links for Glk1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Glk1p

1)	Hirayama T, et al. (1995) Cloning and characterization of seven cDNAs for hyperosmolarity-responsive (HOR) genes of Saccharomyces cerevisiae. Mol Gen Genet 249(2):127-38
2)	Herrero P, et al. (1995) Transcriptional regulation of the Saccharomyces cerevisiae HXK1, HXK2 and GLK1 genes. Yeast 11(2):137-44
3)	Rodriguez A, et al. (2001) The hexokinase 2 protein regulates the expression of the GLK1, HXK1 and HXK2 genes of Saccharomyces cerevisiae. Biochem J 355(Pt 3):625-31
4)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41