GLK1/YCL040W Protein Information Help

Standard Name Glk1p
Systematic Name Ycl040wp
Alias Hor3p 1
ORF Classification Verified
Description Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication (2, 3, 4)
Name Description GLucoKinase
Experimental Data
Molecules/cell 21100 5
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 500
Molecular Weight (Da) 55,377
Isoelectric Point (pI) 6.14

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Glk1p (InterPro)
Physical Interactions There are 21 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000012CFB3 | P17709
MIPS: YCL040W
NCBI: 123899 | 5323 | 577521 | 6319809 | NP_009890.1 | NM_001178685.1
GenBank/EMBL/DDBJ: AAA53536.1 | CAA42376.1 | DAA07444.1 | M24077 | X59720
External Classifications EC: 2.7.1.2 [Glucokinase]
Amino Acid Sequence (or in FASTA format)
       1  MSFDDLHKAT ERAVIQAVDQ ICDDFEVTPE KLDELTAYFI EQMEKGLAPP
      51  KEGHTLASDK GLPMIPAFVT GSPNGTERGV LLAADLGGTN FRICSVNLHG
     101  DHTFSMEQMK SKIPDDLLDD ENVTSDDLFG FLARRTLAFM KKYHPDELAK
     151  GKDAKPMKLG FTFSYPVDQT SLNSGTLIRW TKGFRIADTV GKDVVQLYQE
     201  QLSAQGMPMI KVVALTNDTV GTYLSHCYTS DNTDSMTSGE ISEPVIGCIF
     251  GTGTNGCYME EINKITKLPQ ELRDKLIKEG KTHMIINVEW GSFDNELKHL
     301  PTTKYDVVID QKLSTNPGFH LFEKRVSGMF LGEVLRNILV DLHSQGLLLQ
     351  QYRSKEQLPR HLTTPFQLSS EVLSHIEIDD STGLRETELS LLQSLRLPTT
     401  PTERVQIQKL VRAISRRSAY LAAVPLAAIL IKTNALNKRY HGEVEIGCDG
     451  SVVEYYPGFR SMLRHALALS PLGAEGERKV HLKIAKDGSG VGAALCALVA
     501  *                                                     

external links for Glk1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) Organelle DB
Aspergillus (AspGD) CYC2008 (complexes) MIPS YPL+
Candida (CGD) Complexome Pfam domains YeastGFP
YGOB DIP YeastRC Structure Prediction (Seattle) YeastRC Public Image Repository
YOGY GeneMANIA


IMP

References cited on this page View Complete Literature Guide for Glk1p
1) Hirayama T, et al.  (1995) Cloning and characterization of seven cDNAs for hyperosmolarity-responsive (HOR) genes of Saccharomyces cerevisiae. Mol Gen Genet 249(2):127-38
2) Herrero P, et al.  (1995) Transcriptional regulation of the Saccharomyces cerevisiae HXK1, HXK2 and GLK1 genes. Yeast 11(2):137-44
3) Rodriguez A, et al.  (2001) The hexokinase 2 protein regulates the expression of the GLK1, HXK1 and HXK2 genes of Saccharomyces cerevisiae. Biochem J 355(Pt 3):625-31
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41