STE50/YCL032W Protein Information

Standard Name	Ste50p 1
Systematic Name	Ycl032wp
ORF Classification	Verified
Description	Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction (2, 3)
Name Description	STErile 4

Experimental Data
Molecules/cell	1670 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	346
Molecular Weight (Da)	38,970
Isoelectric Point (pI)	5.22

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ste50p (InterPro)
Physical Interactions	There are 143 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000136073 \| P25344 MIPS: YCL032W NCBI: 134941 \| 159163169 \| 264803 \| 3313 \| 5332 \| 6319817 \| 90108577 \| NP_009898.1 GenBank/EMBL/DDBJ: DAA07452.1 \| S54312 \| X59720 \| Z11116

Amino Acid Sequence (or in FASTA format)
1 MEDGKQAINE GSNDASPDLD VNGTILMNNE DFSQWSVDDV ITWCISTLEV 51 EETDPLCQRL RENDIVGDLL PELCLQDCQD LCDGDLNKAI KFKILINKMR 101 DSKLEWKDDK TQEDMITVLK NLYTTTSAKL QEFQSQYTRL RMDVLDVMKT 151 SSSSSPINTH GVSTTVPSSN NTIIPSSDGV SLSQTDYFDT VHNRQSPSRR 201 ESPVTVFRQP SLSHSKSLHK DSKNKVPQIS TNQSHPSAVS TANTPGPSPN 251 EALKQLRASK EDSCERILKN AMKRHNLADQ DWRQYVLVIC YGDQERLLEL 301 NEKPVIIFKN LKQQGLHPAI MLRRRGDFEE VAMMNGSDNV TPGGRL*

Amino Acid Sequence (or in FASTA format)

       1  MEDGKQAINE GSNDASPDLD VNGTILMNNE DFSQWSVDDV ITWCISTLEV
      51  EETDPLCQRL RENDIVGDLL PELCLQDCQD LCDGDLNKAI KFKILINKMR
     101  DSKLEWKDDK TQEDMITVLK NLYTTTSAKL QEFQSQYTRL RMDVLDVMKT
     151  SSSSSPINTH GVSTTVPSSN NTIIPSSDGV SLSQTDYFDT VHNRQSPSRR
     201  ESPVTVFRQP SLSHSKSLHK DSKNKVPQIS TNQSHPSAVS TANTPGPSPN
     251  EALKQLRASK EDSCERILKN AMKRHNLADQ DWRQYVLVIC YGDQERLLEL
     301  NEKPVIIFKN LKQQGLHPAI MLRRRGDFEE VAMMNGSDNV TPGGRL*

external links for Ste50p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Ste50p

1)	Rad MR, et al. (1991) The complete sequence of a 11,953 bp fragment from C1G on chromosome III encompasses four new open reading frames. Yeast 7(5):533-8
2)	Wu C, et al. (1999) Functional characterization of the interaction of Ste50p with Ste11p MAPKKK in Saccharomyces cerevisiae. Mol Biol Cell 10(7):2425-40
3)	Ramezani-Rad M (2003) The role of adaptor protein Ste50-dependent regulation of the MAPKKK Ste11 in multiple signalling pathways of yeast. Curr Genet 43(3):161-70
4)	Rad MR, et al. (1992) STE50, a novel gene required for activation of conjugation at an early step in mating in Saccharomyces cerevisiae. Mol Gen Genet 236(1):145-54
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41