SGF29/YCL010C Protein Information

Standard Name	Sgf29p 1
Systematic Name	Ycl010cp
ORF Classification	Verified
Description	Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation (1, 2, 3, 4)
Name Description	SaGa associated Factor 1

Experimental Data
Molecules/cell	1750 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	259
Molecular Weight (Da)	29,381
Isoelectric Point (pI)	5.23

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Sgf29p (InterPro)
Physical Interactions	There are 188 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000053131 \| P25554 MIPS: YCL010C NCBI: 1907134 \| 332138136 \| 332138138 \| 332138140 \| 6319836 \| 6686346 \| NP_009917.1 GenBank/EMBL/DDBJ: DAA07471.1 \| X59720

Amino Acid Sequence (or in FASTA format)
1 MDGYWDVVVS SLQDIYNANE VIPFDDELQT KKLNFLNMSK DQLQLHLNTF 51 QEHMENVNRV HRILDNVRSN LSLMLNQSRE EKSEENTEDA EEGEGTRMAL 101 SQGKKAVGKV GRSYWTSEYN PNAPILVGSE VAYKPRRGSA DGEWIQCEVL 151 KVVADGTRFE VRDPEPDELG NSGKVYKCNR KELLLIPPGF PTKNYPPGTK 201 VLARYPETTT FYPAIVIGTK RDGTCRLRFD GEEEVDKETE VTRRLVLPSP 251 TALANLARK*

Amino Acid Sequence (or in FASTA format)

       1  MDGYWDVVVS SLQDIYNANE VIPFDDELQT KKLNFLNMSK DQLQLHLNTF
      51  QEHMENVNRV HRILDNVRSN LSLMLNQSRE EKSEENTEDA EEGEGTRMAL
     101  SQGKKAVGKV GRSYWTSEYN PNAPILVGSE VAYKPRRGSA DGEWIQCEVL
     151  KVVADGTRFE VRDPEPDELG NSGKVYKCNR KELLLIPPGF PTKNYPPGTK
     201  VLARYPETTT FYPAIVIGTK RDGTCRLRFD GEEEVDKETE VTRRLVLPSP
     251  TALANLARK*

external links for Sgf29p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Sgf29p

1)	Sanders SL, et al. (2002) Proteomics of the eukaryotic transcription machinery: identification of proteins associated with components of yeast TFIID by multidimensional mass spectrometry. Mol Cell Biol 22(13):4723-38
2)	Bian C, et al. (2011) Sgf29 binds histone H3K4me2/3 and is required for SAGA complex recruitment and histone H3 acetylation.LID - 10.1038/emboj.2011.193 [doi] EMBO J ()
3)	Lee KK, et al. (2011) Combinatorial depletion analysis to assemble the network architecture of the SAGA and ADA chromatin remodeling complexes. Mol Syst Biol 7():503
4)	Shukla A, et al. (2012) Sgf29p facilitates the recruitment of TATA box binding protein but does not alter SAGA's global structural integrity in vivo. Biochemistry 51(2):706-14
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41